Probiotic Coating for Ripened Cheeses With Lactobacillus Acidophilus and Lactobacillus Helveticus Inclusion

Edible films have been employed to improve the food quality. Thus, the objective of this study was to evaluate the edible coverage as a vehicle for probiotics bacteria in cheeses. The experimental design was completely randomized containing four treatments: uncoated cheeses, sodium alginate coated cheeses, sodium alginate + Lactobacillus acidophilus coated cheeses and sodium alginate + Lactobacillus helveticus coated cheeses, analysed for 15 days. The parameters of water steam permeability, thickness and Young's modulus were significant. In the simulation of gastrointestinal conditions, there was a reduction in lactic acid bacteria. There was a reduction in coliform values in coated cheeses. In the identification using Random Amplified Polymorphic DNA technique, Lactobacillus helveticus strains were isolated, suggesting the microorganism migration to inside the cheese. Scanning electron microscopy showed that the lactic acid bacteria were distributed throughout the surface of the edible coating. It suggested the Lactobacillus helveticus permeability added in the cover to the cheese interior, ensuring that the cover can be a vehicle for dairy bacteria.

[1]  Wen Qin,et al.  Physical and antimicrobial properties of edible films containing Lactococcus lactis. , 2019, International journal of biological macromolecules.

[2]  B. Bhandari,et al.  The viability of probiotic Lactobacillus rhamnosus (non-encapsulated and encapsulated) in functional reduced-fat cream cheese and its textural properties during storage , 2019, Food Control.

[3]  C. F. Volken de Souza,et al.  Development of alginate-pectin microparticles with dairy whey using vibration technology: Effects of matrix composition on the protection of Lactobacillus spp. from adverse conditions. , 2018, Food research international.

[4]  M. Sabaa,et al.  Biobased alginate/castor oil edible films for active food packaging , 2018, LWT.

[5]  J. Teixeira,et al.  Use of edible films and coatings in cheese preservation: Opportunities and challenges. , 2018, Food research international.

[6]  A. Mortazavian,et al.  Survival of probiotic bacteria in carboxymethyl cellulose-based edible film and assessment of quality parameters , 2018 .

[7]  F. Yamashita,et al.  Development and Active Biodegradable Film Evaluation Incorporated with Oregano Essential Oil and Nanoclay , 2017 .

[8]  A. Madureira,et al.  Edible films as carrier for lactic acid bacteria , 2016 .

[9]  C. Rocha,et al.  Artisanal Coalho cheeses as source of beneficial Lactobacillus plantarum and Lactobacillus rhamnosus strains , 2015 .

[10]  C. Parmenter,et al.  Stability of Lactobacillus rhamnosus GG in prebiotic edible films , 2014, Food chemistry.

[11]  E. L. de Souza,et al.  Addition of probiotic bacteria in a semi-hard goat cheese ( coalho ) : Survival to simulated gastrointestinal conditions and inhibitory effect against pathogenic bacteria , 2014 .

[12]  P. Kanmani,et al.  Development and characterization of novel probiotic-residing pullulan/starch edible films. , 2013, Food chemistry.

[13]  A. Chiralt,et al.  Physical properties and antilisterial activity of bioactive edible films containing Lactobacillus plantarum , 2013 .

[14]  M. Gatti,et al.  Identification of dairy lactic acid bacteria by tRNAAla-23S rDNA-RFLP. , 2012, Journal of microbiological methods.

[15]  F. Malcata,et al.  Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese. , 2012, Journal of dairy science.

[16]  K. Koutsoumanis,et al.  Development of a novel bioactive packaging based on the incorporation of Lactobacillus sakei into sodium-caseinate films for controlling Listeria monocytogenes in foods , 2010 .

[17]  S. Oustan,et al.  Influence of Proteolysis and Soluble Calcium Levels on Textural Changes in the Interior and Exterior of Iranian UF White Cheese during Ripening , 2010 .

[18]  V. Nedović,et al.  Encapsulation of Probiotics for use in Food Products , 2010, ETFA 2010.

[19]  Z. Yuksekdag,et al.  Assessment of potential probiotic- and starter properties of Pediococcus spp. isolated from Turkish-type fermented sausages (sucuk). , 2010, Journal of microbiology and biotechnology.

[20]  F. Shahidi,et al.  The influence of multi stage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice , 2009 .

[21]  M. Gatti,et al.  Application of AFLP fingerprint analysis for studying the biodiversity of Streptococcus thermophilus. , 2009, Journal of microbiological methods.

[22]  A. G. Cruz,et al.  Probiotic cheese: health benefits, technological and stability aspects , 2009 .

[23]  E. Neviani,et al.  Application of molecular approaches to study lactic acid bacteria in artisanal cheeses. , 2009, Journal of microbiological methods.

[24]  F. Patrignani,et al.  Probiotic Crescenza cheese containing Lactobacillus casei and Lactobacillus acidophilus manufactured with high-pressure homogenized milk. , 2008, Journal of dairy science.

[25]  M. Ré,et al.  Microencapsulation of Bifidobacterium animalis subsp. lactis in Modified Alginate-chitosan Beads and Evaluation of Survival in Simulated Gastrointestinal Conditions , 2007 .

[26]  M. Phillips,et al.  Viability of commercial probiotic cultures (L. acidophilus, Bifidobacterium sp., L. casei, L. paracasei and L. rhamnosus) in cheddar cheese. , 2006, International journal of food microbiology.

[27]  M. Hayes,et al.  Cheese as a delivery vehicle for probiotics and biogenic substances , 2006 .

[28]  J. Kwon,et al.  Pectin- and gelatin-based film: effect of gamma irradiation on the mechanical properties and biodegradation , 2005 .

[29]  Christophe Lacroix,et al.  Encapsulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal conditions and in yoghurt , 2004 .

[30]  G. Marcazzan,et al.  Use of RAPD‐PCR and TTGE for the evaluation of biodiversity of whey cultures for Grana Padano cheese , 2004, Letters in applied microbiology.

[31]  M. Sanders Probiotics: Considerations for Human Health , 2003, Nutrition reviews.

[32]  P. Peiris,et al.  Encapsulation of probiotic bacteria with alginate-starch and evaluation of survival in simulated gastrointestinal conditions and in yoghurt. , 2000, International journal of food microbiology.

[33]  E. A. Zottola,et al.  Growth and Survival of Undesirable Bacteria in Cheese , 1993 .

[34]  N. Gontard,et al.  Water and Glycerol as Plasticizers Affect Mechanical and Water Vapor Barrier Properties of an Edible Wheat Gluten Film , 1993 .

[35]  F. Rohlf,et al.  NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, version 2.1: Owner manual , 1992 .

[36]  A. Rao,et al.  SURVIVAL OF MICROENCAPSULATED BIFIDOBACTERIUM PSEUDOLONGUM IN SIMULATED GASTRIC AND INTESTINAL JUICES , 1989 .