Levels of creatine kinase and its BB isoenzyme in lung cancer specimens and cultures.

Small-cell carcinomas of the lung (SCCL) have properties of amine-handling cells, and high levels of the key amine-handling cell enzyme L-dopa decarboxylase (EC 4.1.1.28) distinguish SCCL from most other lung cancers. SCCL tumor specimens and continuous cultures also are characterized by high levels of creatine kinase (EC 2.7.3.2) and its BB isoenzyme (CK-BB). Electrophoretic analysis of creatine kinase isoenzymes indicated that creatine kinase levels in SCCL were quantitatively but not qualitatively different from those in normal lung and other lung cancers. Supernatant fluids of SCCL cultures contained relatively modest concentrations of CK-BB but lacked detectable L-dopa decarboxylase activity. Variant SCCL cultures with altered morphology lost their amine-handling properties, including L-dopa decarboxylase activity, but retained high levels of CK-BB, indicating discordant expression of the two enzymes. CK-BB levels were measured in the sera of 67 patients having SCCL. Elevated levels were present in 16 of 41 patients (39%) having extensive-stage disease but in none of 26 patients (0%) having limited-stage disease.

[1]  W. Decoteau,et al.  Creatine kinase isoenzymes in testicular tissue of normal subjects and in a case of lymphoblastic lymphosarcoma. , 1980, Clinical Chemistry.

[2]  A. Gazdar,et al.  Activities of L-dopa decarboxylase and diamine oxidase (histaminase) in human lung cancers and decarboxylase as a marker for small (oat) cell cancer in cell culture. , 1980, Cancer research.

[3]  A. Churg,et al.  Small cell squamous and mixed small cell squamous—small cell anaplastic carcinomas of the lung , 1980, The American journal of surgical pathology.

[4]  M. Zweig,et al.  The presence of creatine kinase BB isoenzyme in patients with prostatic cancer. , 1980, Clinica chimica acta; international journal of clinical chemistry.

[5]  J. Nosanchuk,et al.  Elevation of brain‐type creatine kinase in serum from patients with carcinoma , 1979, Cancer.

[6]  D. Ettinger,et al.  Changes in morphologic and biochemical characteristics of small cell carcinoma of the lung. A clinicopathologic study. , 1979, The American journal of medicine.

[7]  S. Weinhouse,et al.  Creatine kinase activity and isozyme composition in normal tissues and neoplasms of rats and mice. , 1979, Cancer research.

[8]  R. Makuch,et al.  Cyclic alternating combination chemotherapy for small cell bronchogenic carcinoma. , 1979, Cancer treatment reports.

[9]  D. Yaffe,et al.  Expression of creatine kinase isoenzymes in myogenic cell lines. , 1979, Developmental biology.

[10]  V. Saks,et al.  Role of creatine phosphokinase in cellular function and metabolism. , 1978, Canadian journal of physiology and pharmacology.

[11]  D. Ettinger,et al.  Variable content of histaminase, L-dopa decarboxylase and calcitonin in small-cell carcinoma of the lung. Biologic and clinical implications. , 1978, The New England journal of medicine.

[12]  S. Baylin,et al.  Association of diamine oxidase and ornithine decarboxylase with maturing cells in rapidly proliferating epithelium. , 1978, Biochimica et biophysica acta.

[13]  W. Gerhardt,et al.  Creatine kinase in serum: 3. Further study of adenylate kinase inhibitors. , 1977, Clinical chemistry.

[14]  M. Matsuda,et al.  Small cell carcinoma of the lung: clinical and cytomorphological studies in relation to its response to chemotherapy. , 1977, Gan.

[15]  S. Tsung.  Creatine kinase isoenzyme patterns in human tissue obtained at surgery. , 1976, Clinical chemistry.

[16]  A. Pearse,et al.  THE CYTOCHEMISTRY AND ULTRASTRUCTURE OF POLYPEPTIDE HORMONE-PRODUCING CELLS OF THE APUD SERIES AND THE EMBRYOLOGIC, PHYSIOLOGIC AND PATHOLOGIC IMPLICATIONS OF THE CONCEPT , 1969, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[17]  H. B. Mann,et al.  On a Test of Whether one of Two Random Variables is Stochastically Larger than the Other , 1947 .

[18]  T. Burt,et al.  July , 1890, The Hospital.

[19]  A. Gazdar,et al.  Pathology of Small Cell Carcinoma of The Lung and Its Subtypes. A Clinico-Pathologic Correlation , 1981 .

[20]  A. Gazdar,et al.  Small cell carcinoma of the lung: altered morphological, bio- logical and biochemical characteristics in long term cultures and heterotransplanted tumors. Abstr. , 1980 .

[21]  A. Schechter,et al.  Radioimmunoassay of creatine kinase isoenzymes in human serum: isoenzyme BB. , 1978, Clinical chemistry.

[22]  G. Hoag,et al.  The production of creatine kinase isozyme BB in sera of a patient with prostatic carcinoma and in tumor homogenates. , 1978, Clinical biochemistry.

[23]  R. Weitzman,et al.  Ectopic peptide synthesis: a universal concomitant of neoplasia. , 1977, Transactions of the Association of American Physicians.

[24]  G. Pfleiderer,et al.  Quantitation of creatine kinase isoenzymes in human tissues and sera by an immunological method. , 1975, Clinica chimica acta; international journal of clinical chemistry.

[25]  S. Jacobsen,et al.  A new assay for histaminase activity: measurement of tritiated water from beta (side chain label)-H 3-histamine. , 1971, The Journal of pharmacology and experimental therapeutics.