Isolated compounds from black bean inhibit the classical pathway of the complement.

The present study evaluated the anticomplement effects from isolated compounds of black bean in classical pathway complement system. Using column chromatograph, four compounds kaempferol 3-O-β-D-glucopyranoside (1), kaempferol 5-O-β-D-glucopyranoside (2), 3-O-[α-L-rhamnopyranosyl(1-2)-β-D-glucopyranosyl(1-2)-β-D-glucuronopyranosyl]olean-12-en-3β,22β,24-triol (3) and 3-O-[β-D-glucopyranosyl(1-2)-β-D-galactopyranosyl(1-2)-β-D-glucuronopyranosyl]olean-12-en-3β,22β,24-triol (4) were isolated and evaluated for in vitro anticomplement activity. 3 showed inhibitory activity against complement system with 50% inhibitory concentrations (IC(50)) values of 98.2 μg/ml. This is the first report of anticomplement activity of isolated compounds from black bean.

[1]  Eunhye Kim,et al.  Inhibition effects of the classical pathway complement of isolated compounds from Quercus glauca , 2011, Human & experimental toxicology.

[2]  Young-Choon Lee,et al.  Inhibitory Effects of Isolated Compounds from Black Coloured Rice Bran on the Complement Classical Pathway , 2011, Phytotherapy research : PTR.

[3]  Myong-Jo Kim,et al.  RETRACTED: Inhibition effects of the classical pathway complement of three Sorghum bicolor from South Korea , 2011, Immunopharmacology and immunotoxicology.

[4]  H. Moon,et al.  Anti‐complement activity of isolated compounds from the roots of Clerodendrum bungei Steud. , 2010, Phytotherapy research : PTR.

[5]  Dong-Hyun Kim,et al.  Anti-inflammatory effects of black rice, cyanidin-3-O-beta-D-glycoside, and its metabolites, cyanidin and protocatechuic acid. , 2010, International immunopharmacology.

[6]  T. Sugahara,et al.  White Sorghum (Sorghum bicolor (L.) Moench) Bran Extracts Suppressed IgE Production by U266 Cells , 2009, Bioscience, biotechnology, and biochemistry.

[7]  Xiangjiu He,et al.  Phytochemicals of black bean seed coats: isolation, structure elucidation, and their antiproliferative and antioxidative activities. , 2007, Journal of agricultural and food chemistry.

[8]  M. Lila,et al.  Chemopreventive activity of polyphenolics from black Jamapa bean (Phaseolus vulgaris L.) on HeLa and HaCaT cells. , 2006, Journal of agricultural and food chemistry.

[9]  Jung-hee Kim,et al.  Anti-complement activity of constituents from the stem-bark of Juglans mandshurica. , 2003, Biological & pharmaceutical bulletin.

[10]  I. Delgadillo,et al.  Effect of Grain Structure and Cooking on Sorghum and Maize in vitro Protein Digestibility , 2002 .

[11]  M. Ogundele.  Anti-complement activities of human breast-milk , 1999, Inflammation Research.

[12]  K. Ahn,et al.  Anti-complement activity of tiliroside from the flower buds of Magnolia fargesii. , 1998, Biological & pharmaceutical bulletin.

[13]  Mahabir P. Gupta,et al.  Complement-Inhibiting Properties of Apeiba tibourbou , 1993, Planta medica.

[14]  L. Hangen,et al.  Consumption of black beans and navy beans (Phaseolus vulgaris) reduced azoxymethane-induced colon cancer in rats. , 2002 .