Morphology and structure of silkworm cocoons

Abstract Silkworm cocoons are natural polymer fibre composites made from silk fibres and sericin binder. While silk is an interesting natural material per se , an understanding of the role of silk within one of its main functional applications in silkworm cocoons will provide inspiration and tools for the design of new artificial silk composites. Here, we describe in some detail the structure and morphology of the cocoons of 27 different species of silkworm. While cocoon morphology can be described very broadly as a nonwoven fibre composite, we demonstrate a diversity of structural features such as: the number and connectivity of layers through the cocoon wall thickness, the amount and distribution of sericin binder, the diameter and packing density of the silk fibres, the degree of orientation of the nonwoven structure, the distribution of larger holes within that structure, and the presence of calcium oxalate crystals.

[1]  Narendra Reddy,et al.  Structure and properties of cocoons and silk fibers produced by Hyalophora cecropia , 2010 .

[2]  W. Burggren,et al.  The silk cocoon of the silkworm, Bombyx mori: macro structure and its influence on transmural diffusion of oxygen and water vapor. , 2010, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology.

[3]  Xi-Qiao Feng,et al.  Mechanical properties of silkworm cocoons , 2005 .

[4]  Hongping Zhao,et al.  Variability in mechanical properties of Bombyx mori silk , 2007 .

[5]  Fritz Vollrath,et al.  Silk as a Biomimetic Ideal for Structural Polymers , 2009 .

[6]  Fritz Vollrath,et al.  Silks as ancient models for modern polymers , 2009 .

[7]  T. Ma,et al.  Thermal compression and characterization of three-dimensional nonwoven PET matrices as tissue engineering scaffolds. , 2001, Biomaterials.

[8]  H. Danks The roles of insect cocoons in cold conditions , 2004 .

[9]  Hongping Zhao,et al.  Mechanical properties of silkworm cocoon pelades , 2007 .

[10]  J. C. Regier,et al.  A phylogenetic study of the ‘bombycoid complex’ (Lepidoptera) using five protein‐coding nuclear genes, with comments on the problem of macrolepidopteran phylogeny , 2008 .

[11]  M. M. Collins,et al.  The Wild Silk Moths of North America : A Natural History of the Saturniidae of the United States and Canada , 1996 .

[12]  W. Kloot,et al.  Cocoon Construction By the Cecropia Silkworm I. the Role of the External Environment , 1953 .

[13]  Jinrong Yao,et al.  The preparation of high performance silk fiber/fibroin composite , 2010 .

[14]  R. S. Peigler,et al.  Phylogenetic relationships of wild silkmoths (Lepidoptera: Saturniidae) inferred from four protein‐coding nuclear genes , 2008 .

[15]  G. Freddi,et al.  Chemical Structure and Physical Properties of Antheraea assama Silk. , 1994 .

[16]  Thomas Scheibel,et al.  Composite materials based on silk proteins , 2010 .

[17]  L. Trouvelot The American Silk Worm , 1867, The American Naturalist.

[18]  J. Church,et al.  An unlikely silk: the composite material of green lacewing cocoons. , 2008, Biomacromolecules.

[19]  Fujia Chen,et al.  Silkworm cocoons inspire models for random fiber and particulate composites. , 2010, Physical review. E, Statistical, nonlinear, and soft matter physics.

[20]  R. S. Peigler,et al.  Monophyly, composition, and relationships within Saturniinae (Lepidoptera: Saturniidae): Evidence from two nuclear genes , 2002 .

[21]  L. P. Lounibos The cocoon spinning behaviour of the chinese oak silkworm, Antheraea pernyi , 1975, Animal Behaviour.

[22]  David L Kaplan,et al.  Ingrowth of human mesenchymal stem cells into porous silk particle reinforced silk composite scaffolds: An in vitro study. , 2011, Acta biomaterialia.

[23]  W. Kloot,et al.  COCOON CONSTRUCTION BY THE CECROPIA SILKWORM III. THE ALTERATION OF SPINNING BEHAVIOR BY CHEMICAL AND SURGICAL TECHNIQUES , 1954 .

[24]  L. Wang,et al.  Preparation and physicochemical properties of a novel hydroxyapatite/chitosan–silk fibroin composite , 2007 .