Bioelectrocatalytic fructose oxidation with fructose dehydrogenase-bearing conducting polymer films for biofuel cell application

Abstract This study presents an enzymatic bioanode fabricated with fructose dehydrogenase and a polyaniline film to construct a single-compartment fructose biofuel cell. The enzymatic bioanode provided fructose oxidation current, which accompanied the electron transfer between the heme c moiety of fructose dehydrogenase and polyaniline. Characterization of the bioanode at a pH of 4.5 indicated an onset potential of − 0.1 V (vs. Ag/AgCl) with respect to the redox potential corresponding to heme c of fructose dehydrogenase as well as high current densities for fructose oxidation of 1.0 ± 0.1 mA/cm2 at + 0.50 V (vs. Ag/AgCl). A single-compartment fructose biofuel cell was constructed by use of the bioanode together with an enzymatic biocathode fabricated with laccase and polythiophene copolymer film. The fructose biofuel cell possessed an open-circuit potential of 0.55 V with an associated short-circuit current of 1.4 ± 0.2 mA/cm2. In addition, the maximum power density of the biofuel cell was 0.36 ± 0.04 mW/cm2 at a cell voltage of 0.3 V.

[1]  Takashi Kuwahara,et al.  Fabrication of enzyme electrodes with a polythiophene derivative and application of them to a glucose fuel cell , 2009 .

[2]  M. Shimomura,et al.  Electrochemical polymerization of aniline in the presence of poly(acrylic acid) and characterization of the resulting films , 2012 .

[3]  I. Taniguchi,et al.  D-fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode. , 2009, Biosensors & bioelectronics.

[4]  Shelley D Minteer,et al.  Contact lens biofuel cell tested in a synthetic tear solution. , 2015, Biosensors & bioelectronics.

[5]  M. Shimomura,et al.  Bioelectrocatalytic O(2) reduction with a laccase-bearing poly(3-methylthiophene) film based on direct electron transfer from the polymer to laccase. , 2013, Bioelectrochemistry.

[6]  N. Mano,et al.  A membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes. , 2015, Chemical communications.

[7]  O. Shirai,et al.  Dual gas-diffusion membrane- and mediatorless dihydrogen/air-breathing biofuel cell operating at room temperature , 2016 .

[8]  E. Katz,et al.  Implanted biofuel cell operating in a living snail. , 2012, Journal of the American Chemical Society.

[9]  Michael Holzinger,et al.  Direct electron transfer between tyrosinase and multi-walled carbon nanotubes for bioelectrocatalytic oxygen reduction , 2012 .

[10]  F. Gao,et al.  Mediatorless glucose biosensor and direct electron transfer type glucose/air biofuel cell enabled with carbon nanodots. , 2015, Analytical chemistry.

[11]  Shelley D. Minteer,et al.  Membraneless enzymatic ethanol/O-2 fuel cell: Transitioning from an air-breathing Pt-based cathode to a bilirubin oxidase-based biocathode , 2016 .

[12]  Antonio Facchetti,et al.  π-Conjugated Polymers for Organic Electronics and Photovoltaic Cell Applications† , 2011 .

[13]  M. Shimomura,et al.  Bioelectrocatalytic O2 reduction with a laccase-bearing film of the copolymer of 3-methylthiophene and thiophene-3-acetic acid , 2016 .

[14]  T. Kyotani,et al.  Improving the Direct Electron Transfer in Monolithic Bioelectrodes Prepared by Immobilization of FDH Enzyme on Carbon-Coated Anodic Aluminum Oxide Films , 2016, Front. Mater..

[15]  C. Avignone-Rossa,et al.  A one-compartment fructose/air biological fuel cell based on direct electron transfer. , 2009, Biosensors & bioelectronics.

[16]  Kenji Kano,et al.  A high-power glucose/oxygen biofuel cell operating under quiescent conditions , 2009 .

[17]  E. Katz,et al.  Conjugated polymers ‐ carbon nanotubes‐based functional materials for organic photovoltaics: a critical review , 2012 .

[18]  Kenji Kano,et al.  The electron transfer pathway in direct electrochemical communication of fructose dehydrogenase with electrodes , 2014 .

[19]  Amay J Bandodkar,et al.  Non-invasive wearable electrochemical sensors: a review. , 2014, Trends in biotechnology.

[20]  Seon Jeong Kim,et al.  High-power biofuel cell textiles from woven biscrolled carbon nanotube yarns , 2014, Nature Communications.

[21]  F. Gao,et al.  Engineering hybrid nanotube wires for high-power biofuel cells. , 2010, Nature communications.

[22]  S. Cosnier,et al.  High power enzymatic biofuel cell based on naphthoquinone-mediated oxidation of glucose by glucose oxidase in a carbon nanotube 3D matrix. , 2013, Physical chemistry chemical physics : PCCP.

[23]  I. Taniguchi,et al.  Direct heterogeneous electron transfer reactions and molecular orientation of fructose dehydrogenase adsorbed onto pyrolytic graphite electrodes , 2007 .

[24]  Michelle A. Rasmussen,et al.  An implantable biofuel cell for a live insect. , 2012, Journal of the American Chemical Society.

[25]  S. Tsujimura,et al.  Effect of Pore Size of MgO-templated Carbon on the Direct Electrochemistry of D -fructose Dehydrogenase , 2015 .

[26]  Michael Holzinger,et al.  Carbon nanotube/enzyme biofuel cells , 2012 .

[27]  Philippe Cinquin,et al.  Mediatorless high-power glucose biofuel cells based on compressed carbon nanotube-enzyme electrodes , 2011, Nature communications.

[28]  O. Shirai,et al.  Interaction between d-fructose dehydrogenase and methoxy-substituent-functionalized carbon surface to increase productive orientations , 2016 .

[29]  K. Matsushita,et al.  D-fructose dehydrogenase of Gluconobacter industrius: purification, characterization, and application to enzymatic microdetermination of D-fructose , 1981, Journal of bacteriology.

[30]  M. Shimomura,et al.  A novel system combining biocatalytic dephosphorylation of L-ascorbic acid 2-phosphate and electrochemical oxidation of resulting ascorbic acid. , 2011, Biosensors & bioelectronics.