Screening of single Escherichia coli by electric field and laser tweezer

In this paper, we propose a novel methodology for high throughput screening of microbes. We have developed a prototype of a microchannel system for high throughput screening of Escherichia coli. Experimental demonstrations of noncontact transportation and manipulation of Escherichia coli by dielectrophoretic force and radiation pressure of laser tweezers were carried out with a laser manipulator system. We discuss research issues in bio-micromanipulation and present a new direction in this field. We consider the basic strategies to improve the working efficiency and the operability of bio-micromanipulation. In experiments, we show that transportation and separation of Escherichia coli with electric field and optical radiation pressure is profitable for future practical application in high throughput screening of microbes.

[1]  W. Benecke,et al.  Linear motion of dielectric particles and living cells in microfabricated structures induced by traveling electric fields , 1991, [1991] Proceedings. IEEE Micro Electro Mechanical Systems.

[2]  Fumihito Arai,et al.  Microflow system and transportation of DNA molecule by dielectrophoretic force utilizing the conformational transition in the higher order structure of DNA molecule , 1997, Proceedings IEEE The Tenth Annual International Workshop on Micro Electro Mechanical Systems. An Investigation of Micro Structures, Sensors, Actuators, Machines and Robots.

[3]  Fumihito Arai,et al.  Micro manipulation based on micro physics-strategy based on attractive force reduction and stress measurement , 1995, Proceedings 1995 IEEE/RSJ International Conference on Intelligent Robots and Systems. Human Robot Interaction and Cooperative Robots.

[4]  Fumihito Arai,et al.  Micro endeffector with micro pyramids and integrated piezoresistive force sensor , 1996, Proceedings of IEEE/RSJ International Conference on Intelligent Robots and Systems. IROS '96.

[5]  M. Washizu.  Manipulation of biological objects in micromachined structures , 1992, [1992] Proceedings IEEE Micro Electro Mechanical Systems.

[6]  Fumihito Arai,et al.  Bio-micromanipulation (new direction for operation improvement) , 1997, Proceedings of the 1997 IEEE/RSJ International Conference on Intelligent Robot and Systems. Innovative Robotics for Real-World Applications. IROS '97.

[7]  A. Ashkin,et al.  Optical trapping and manipulation of single cells using infrared laser beams , 1987, Nature.

[8]  Shinji Katsura,et al.  Handling of single DNA molecule using electric field and laser beam , 1994, Proceedings of 1994 IEEE Industry Applications Society Annual Meeting.

[9]  T. Fukuda,et al.  Acoustic micromanipulation using a multi-electrode transducer , 1996, MHS'96 Proceedings of the Seventh International Symposium on Micro Machine and Human Science.

[10]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[11]  M. Nishioka,et al.  Opto-electrostatic micromanipulation of single cell and DNA molecule , 1995, MHS'95. Proceedings of the Sixth International Symposium on Micro Machine and Human Science.