Quantitative determination of human plasma apolipoprotein A-II by a non competitive enzyme-linked immunosorbent assay.

A noncompetitive enzyme-linked immunosorbent assay (ELISA) for apolipoprotein A-II (ApoA-II) was developed. Microtiter plates were coated with affinity purified antibodies to ApoA-II. After incubation with human plasma, the amount of ApoA-II bound to the coated plate was determined with peroxidase-labeled antibodies to ApoA-II. When pure ApoA-II or delipidated reference plasma was used as standard, a single step delipidation was required in order to unmask some antigenic sites of ApoA-II. However, the underestimated ApoA-II values in untreated samples were shown to be corrected by using intact reference plasma as secondary standard. The average concentration of ApoA-II in normolipidemic plasma was 0.376 g/l.

[1]  P. Alaupovic,et al.  Quantitative determination of human plasma apolipoprotein A-I by a noncompetitive enzyme-linked immunosorbent assay. , 1985, Clinica chimica acta; international journal of clinical chemistry.

[2]  P. Alaupovlc,et al.  Use and significance of reference serum as a secondary standard for electroimmunoassay of apolipoprotein A-I. , 1984, Clinical chemistry.

[3]  K. Hiwada,et al.  Serum apolipoprotein A-I, A-II and B levels and their discriminative values in relatives of patients with coronary artery disease. , 1984, Atherosclerosis.

[4]  M. Rosseneu,et al.  Immunonephelometry of apolipoprotein A-II in plasma. , 1984, Clinical chemistry.

[5]  L. Rudel,et al.  Measurement of apolipoprotein A-I concentration in nonhuman primate serum by enzyme-linked immunosorbent assay (ELISA). , 1983, Journal of lipid research.

[6]  A. Noma,et al.  Plasma lipids and apolipoproteins as discriminators for presence and severity of angiographically defined coronary artery disease. , 1983, Atherosclerosis.

[7]  C. Heuck,et al.  Immunonephelometry of apolipoprotein A-II in hyperlipoproteinemic serum. , 1983, Clinical chemistry.

[8]  L. Carlson,et al.  Concentrations of apolipoproteins B, C-I, C-II, C-III and E in sera from normal men and their relation to serum lipoprotein levels. , 1982, Clinica chimica acta; international journal of clinical chemistry.

[9]  L. Holmquist Quantitation of human serum apolipoprotein B by enzyme immunoassay. , 1982, Clinica chimica acta; international journal of clinical chemistry.

[10]  T. Musliner,et al.  Radioimmunoassay of Apolipoprotein A‐II , 1982, Arteriosclerosis.

[11]  J. Fruchart,et al.  Lipoproteins, apolipoproteins and coronary artery disease , 1982, La Ricerca in clinica e in laboratorio.

[12]  A. J. Valente,et al.  Properties of apolipoprotein B in urea and in aqueous buffers. The use of glutathione and nitrogen in its solubilization. , 1981, Biochimica et biophysica acta.

[13]  L Wilhelmsen,et al.  Multivariate Analyses of Serum Apolipoproteins and Risk Factors in Relation to Acute Myocardial Infarction , 1981, Arteriosclerosis.

[14]  P. Alaupovic,et al.  Quantitative determination of apolipoproteins C-I and C-II in human plasma by separate electroimmunoassays. , 1981, Clinical chemistry.

[15]  R. Goldberg,et al.  Characterization and measurement of human apolipoprotein A-II by radioimmunoassay. , 1980, Journal of lipid research.

[16]  P. Alaupovic,et al.  Quantitative determination of human apolipoprotein C-III by electroimmunoassay. , 1980, Biochimica et biophysica acta.

[17]  W. Hazzard,et al.  High-density lipoproteins in myocardial infarction survivors. , 1978, Metabolism: clinical and experimental.

[18]  S. Olofsson,et al.  Isolation and partial characterization of a new acidic apolipoprotein (apolipoprotein F) from high density lipoproteins of human plasma. , 1978, Biochemistry.

[19]  J. Albers,et al.  The measurement of apolipoprotein A-I and A-II levels in men and women by immunoassay. , 1977, The Journal of clinical investigation.

[20]  P. Alaupovic,et al.  Determination of human apolipoprotein E by electroimmunoassay. , 1976, Biochimica et biophysica acta.

[21]  P. Wahl,et al.  Quantitation of apolipoprotein A-I of human plasma high density lipoprotein. , 1976, Metabolism: clinical and experimental.

[22]  B. Cham,et al.  A solvent system for delipidation of plasma or serum without protein precipitation. , 1976, Journal of lipid research.

[23]  P. Alaupovic,et al.  Determination of apolipoprotein A and its constitutive A-I and A-II polypeptides by separate electroimmunoassays. , 1976, Clinical chemistry.

[24]  E. Engvall,et al.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. , 1972, Journal of immunology.

[25]  P. Alaupovic,et al.  Studies on the composition and structure of plasma lipoproteins. Distribution of lipoprotein families in major density classes of normal human plasma lipoproteins. , 1972, Biochimica et biophysica acta.

[26]  G. Assmann,et al.  Competitive Enzyme Immunoassay for Apolipoprotein A-II , 1983, Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie.

[27]  G. Assmann,et al.  Lipoprotein and apoprotein values in coronary angiography patients. , 1980, Artery.

[28]  W. Riesen,et al.  Electroimmunoassay and Radioimmunoassay for the Quantitation of High Density Apolipoproteins A-I and A-II , 1980, Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie.

[29]  S. Avrameas,et al.  Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. , 1969, Immunochemistry.