Kompetensi Maturasi dan Fertilisasi Oosit Domba Prapubertas Secara In Vitro (DEVELOPMENTAL COMPETENCE OF MATURATION AND FERTILIZATION PREPUBERTAL SHEEP OOCYTES IN VITRO)

The objective of this study was to evaluate the maturation and fertilization ability of oocytes frompre-pubertal sheep ovary in vitro. Prepubertal ovary were collected based on the absence of corpus luteumor absence of corpus albicans in both of the ovaries. Oocytes were collected by slicing methode. Only theoocytes which categories of homogeneous cytoplasm and compact cumulus cells were used and it wasmatured for 24 hours in CO2 incubator with temperature 39oC. Oocytes fertilized in vitro used post thawedspermatozoa with concentration 5x106 and incubated for 12-14 hours. Oocytes were evaluated on numberof oocytes reached MII and number of PN formation. Result of the experiment revealed that there was nosignificant difference in the percentage of MII oocytes after in vitro maturation (89% vs 90,7%, PA 0,05)between prepubertal sheep and pubertal sheep. Meanwhile, the fertilization rate was significantly lower(PÂ 0,05) in prepubertal sheep oocytes compared to pubertal sheep oocytes (60% vs 77,7%). The incidenceof polispermic fertilization was higher in prepubertal sheep oocytes than pubertal sheep oocytes (21,8% vs7,4%) (PA0,05). In conclusion, prepubertal sheep oocytes and pubertal sheep oocytes have similar in vitromaturation ability, even though the ability to be fertilized of the prepubertal sheep oocytes is lower thanthe pubertal sheep.

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