Correlation between the high-frequency content of the QRS on murine surface electrocardiogram and the sympathetic nerves density in left ventricle after myocardial infarction: Experimental study.

Denervated post-infarct scar is arrhythmogenic. Our aim was to compare QRS frequency content in denervated and innervated left ventricular (LV) scar. In-vivo single lead ECG telemetry device was implanted in 17 heterozygous PTPσ (HET) and 7 lacking PTPσ (KO) transgenic mice. Myocardial infarction (MI) with reperfusion and sham surgery was performed. HET mice developed a denervated scar, whereas KO mice developed innervated scar. The power spectral density was used to assess the QRS frequency content. Denervated as compared to innervated post-MI scar was characterized by the higher relative contribution of 300-500 Hz (14 ± 1 vs. 9 ± 1%; P = 0.001) but reduced relative contribution of 200-300 Hz (86 ± 1 vs. 91 ± 1%; P = 0.001). Norepinephrine concentration in peri-infarct zone correlated with both 1-200 Hz (r = 0.75; P = 0.03) and 200-500 Hz QRS power (r = 0.73; P = 0.04). Sympathetic fiber density within the infarct correlated with 200-300/200-500 Hz QRS power ratio (r = 0.56; P = 0.005). Intracellular sigma peptide injections in post-MI HET mice restored the QRS power.

[1]  Agustin Castellanos,et al.  CLINICAL CARDIAC ELECTROPHYSIOLOGY: TECHNIQUES AND INTERPRETATIONS , 1980 .

[2]  S. Solomon,et al.  Sudden death in patients with myocardial infarction and left ventricular dysfunction, heart failure, or both. , 2005, The New England journal of medicine.

[3]  Mark E Josephson,et al.  Frequency content and characteristics of ventricular conduction. , 2015, Journal of electrocardiology.

[4]  S Abboud,et al.  High frequency electrocardiography using an advanced method of signal averaging for non-invasive detection of coronary artery disease in patients with normal conventional electrocardiogram. , 1986, Journal of electrocardiology.

[5]  Ruben Coronel,et al.  Misinterpretation of the mouse ECG: ‘musing the waves of Mus musculus’ , 2014, The Journal of physiology.

[6]  D. Zipes,et al.  Transmural Myocardial Infarction in the Dog Produces Sympathectomy in Noninfarcted Myocardium , 1983, Circulation.

[7]  P. Macfarlane,et al.  Recommendations for standardization and specifications in automated electrocardiography: bandwidth and digital signal processing. A report for health professionals by an ad hoc writing group of the Committee on Electrocardiography and Cardiac Electrophysiology of the Council on Clinical Cardiology, , 1990, Circulation.

[8]  Guy Salama,et al.  Mouse models of long QT syndrome , 2007, The Journal of physiology.

[9]  Mark E. Josephson,et al.  Analysis of speed, curvature, planarity and frequency characteristics of heart vector movement to evaluate the electrophysiological substrate associated with ventricular tachycardia , 2015, Comput. Biol. Medicine.

[10]  H. H. Ros,et al.  Coherent averaging technique: a tutorial review. Part 1: Noise reduction and the equivalent filter. , 1986, Journal of biomedical engineering.

[11]  George B Moody,et al.  Protocol to assess robustness of ST analysers: a case study. , 2004, Physiological measurement.

[12]  Pablo Laguna,et al.  Electrocardiogram (ECG) Signal Processing , 2006 .

[13]  Ruben Coronel,et al.  Early repolarization in mice causes overestimation of ventricular activation time by the QRS duration. , 2013, Cardiovascular research.

[14]  T. Seppänen,et al.  Quantitative beat-to-beat analysis of heart rate dynamics during exercise. , 1996, The American journal of physiology.

[15]  O Pahlm,et al.  Changes in high-frequency QRS components are more sensitive than ST-segment deviation for detecting acute coronary artery occlusion. , 2000, Journal of the American College of Cardiology.

[16]  J. Bruell,et al.  Electrocardiogram of the normal mouse, Mus musculus: general considerations and genetic aspects. , 1968, Cardiovascular research.

[17]  W. Woodward,et al.  Targeting protein tyrosine phosphatase σ after myocardial infarction restores cardiac sympathetic innervation and prevents arrhythmias , 2015, Nature Communications.

[18]  S. Akselrod,et al.  Effects of coronary occlusion on high-frequency content of the epicardial electrogram and body surface electrocardiogram. , 1987, Circulation.

[19]  A. Curtis,et al.  Regional myocardial sympathetic denervation predicts the risk of sudden cardiac arrest in ischemic cardiomyopathy. , 2014, Journal of the American College of Cardiology.

[20]  D. Christini,et al.  Anthropomorphizing the mouse cardiac action potential via a novel dynamic clamp method. , 2009, Biophysical journal.

[21]  J A Vassallo,et al.  Endocardial catheter mapping in patients in sinus rhythm: relationship to underlying heart disease and ventricular arrhythmias. , 1986, Circulation.

[22]  木村 謙介.  Cardiac sympathetic rejuvenation : a link between nerve function and cardiac hypertrophy , 2007 .

[23]  E. Franke,et al.  Study of High Frequency Components in Electrocardiogram by Power Spectrum Analysis , 1962 .

[24]  P. Welch.  The use of fast Fourier transform for the estimation of power spectra: A method based on time averaging over short, modified periodograms , 1967 .

[25]  G. Mitchell,et al.  Measurement of heart rate and Q-T interval in the conscious mouse. , 1998, American journal of physiology. Heart and circulatory physiology.

[26]  Candido Cabo,et al.  Correlation of repolarization of ventricular monophasic action potential with ECG in the murine heart. , 2002, American journal of physiology. Heart and circulatory physiology.

[27]  R. Berger,et al.  Mechanical Alternans Is Associated With Mortality in Acute Hospitalized Heart Failure: Prospective Mechanical Alternans Study (MAS) , 2014, Circulation. Arrhythmia and electrophysiology.

[28]  S. Abboud,et al.  Simulation of high-resolution QRS complex using a ventricular model with a fractal conduction system. Effects of ischemia on high-frequency QRS potentials. , 1991, Circulation research.

[29]  P. Schwartz,et al.  Autonomic nervous system and sudden cardiac death. Experimental basis and clinical observations for post-myocardial infarction risk stratification. , 1992, Circulation.

[30]  B. Habecker,et al.  Infarct-Derived Chondroitin Sulfate Proteoglycans Prevent Sympathetic Reinnervation after Cardiac Ischemia-Reperfusion Injury , 2013, The Journal of Neuroscience.

[31]  Elad Anter,et al.  Substrate Mapping for Ventricular Tachycardia: Assumptions and Misconceptions. , 2015, JACC. Clinical electrophysiology.

[32]  Godfrey L. Smith,et al.  QT interval variability in body surface ECG: measurement, physiological basis, and clinical value: position statement and consensus guidance endorsed by the European Heart Rhythm Association jointly with the ESC Working Group on Cardiac Cellular Electrophysiology. , 2016, Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology.

[33]  D. Bernstein,et al.  Abnormal cardiac function associated with sympathetic nervous system hyperactivity in mice. , 2002, American journal of physiology. Heart and circulatory physiology.