Analysis of proteins by CE, CIEF, and microfluidic devices with whole-column-imaging detection.

The recently developed whole-column-imaging detection technique for capillary electrophoresis (CE) and capillary isoelectric focusing (CIEF), a commercial whole-column-imaged CIEF instrument and its standard operation protocol are introduced. Furthermore, new developments and applications of whole-column-imaging detection in protein-protein interaction study, in protein separation using microfluidic devices and CIEF methods without carrier ampholytes, as well as in 2D separation techniques are reviewed. Miniaturization of whole-column-imaging CIEF and axially illuminated fluorescence whole-column-imaging CIEF are also discussed.

[1]  Stellan Hjertén,et al.  Adaptation of the equipment for high-performance electrophoresis to isoelectric focusing , 1985 .

[2]  J. Pawliszyn,et al.  IMAGING DETECTION METHODS FOR CAPILLARY ISOELECTRIC FOCUSING , 1994 .

[3]  J. Pawliszyn,et al.  Capillary isoelectric focusing with a universal concentration gradient imaging system using a charge-coupled photodiode array , 1992 .

[4]  Andreas Manz,et al.  Chip-based microsystems for genomic and proteomic analysis , 2000 .

[5]  Sam F. Y. Li.  Capillary Electrophoresis: Principles, Practice and Applications , 1992 .

[6]  J. Pawliszyn,et al.  Comprehensive two dimensional separation based on coupling micellar electrokinetic chromatography with capillary isoelectric focusing. , 2002, The Analyst.

[7]  J. Pawliszyn,et al.  Absorption spectra and multicapillary imaging detection for capillary isoelectric focusing using a charge coupled device camera , 1995 .

[8]  Janusz Pawliszyn,et al.  Dual Detection for Capillary Isoelectric Focusing with Refractive Index Gradient and Absorption Imaging Detectors , 1994 .

[9]  J. Pawliszyn,et al.  Capillary isoelectric focusing with whole column detection and a membrane sample preparation system , 1999 .

[10]  J. Pawliszyn,et al.  Capillary zone electrophoresis in agarose gels using absorption imaging detection , 1996, Electrophoresis.

[11]  Stephen C. Jacobson,et al.  Open channel electrochromatography on a microchip , 1994 .

[12]  J. Pawliszyn,et al.  Axially illuminated fluorescence imaging detection for capillary isoelectric focusing on Teflon capillary , 2000 .

[13]  J. Pawliszyn,et al.  Diode laser-based concentration gradient imaging detector for capillary isoelectric focusing , 1995 .

[14]  K. Klepárník,et al.  Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries , 2000, Electrophoresis.

[15]  J. Pawliszyn,et al.  Capillary isoelectric focusing with whole column imaging detection for analysis of proteins and peptides. , 1999, Journal of biochemical and biophysical methods.

[16]  D. J. Harrison,et al.  Capillary electrophoresis and sample injection systems integrated on a planar glass chip , 1992 .

[17]  Janusz Pawliszyn,et al.  Demonstration of isoelectric focusing on an etched quartz chip with UV absorption imaging detection , 1999 .

[18]  J. Pawliszyn,et al.  Determination of isoelectric point and investigation of immunoreaction in peanut allergenic proteins–rabbit IgG antibody system by whole-column imaged capillary isoelectric focusing , 2001 .

[19]  C. Effenhauser,et al.  Integrated capillary electrophoresis on flexible silicone microdevices:  analysis of DNA restriction fragments and detection of single DNA molecules on microchips. , 1997, Analytical chemistry.

[20]  J. Pawliszyn,et al.  Laser‐induced fluorescence detection of non‐covalently labeled protein in capillary isoelectric focusing , 2002 .

[21]  Janusz Pawliszyn,et al.  Universal detection for capillary isoelectric focusing without mobilization using concentration gradient imaging system , 1992 .