A Novel Messenger Ribonucleic Acid Homologous to Human MAGE-D Is Strongly Expressed in Rat Sertoli Cells and Weakly in Leydig Cells and Is Regulated by Follitropin, Lutropin, and Prolactin.

We have cloned a novel complementary DNA whose expression was decreased in rat Sertoli cell cultures after treatment with FSH. This complementary DNA encodes a protein of 570 amino acids and shares 92% homology with the human MAGE-D protein. In contrast to other MAGE genes (A, B, or C), we have shown that MAGE-D expression was ubiquitous in healthy rat tissues. In the seminiferous tubules, the MAGE-D was expressed in Sertoli cells but not in germ cells as demonstrated by RT-PCR and in situ hybridization, whereas for the other MAGE genes, expression has been shown to be restricted to germ cells. Interestingly, MAGE-D was also detected for the first time in the female gonad by Northern blotting. In MLTC-1 cells (mouse Leydig tumor cell line-1), LH and PRL stimulated MAGE-D expression. Using hypophysectomized rats, it was confirmed that FSH decreased MAGE-D expression, whereas LH and PRL increased MAGE-D messenger RNA level in the whole testis most probably through a direct action on Leydig cells. As MAGE-D ...

[1]  R. Nicholls.  Incriminating gene suspects, Prader-Willi style , 1999, Nature Genetics.

[2]  E. Reiter,et al.  A Novel Gene Overexpressed in the Prostate of Castrated Rats: Hormonal Regulation, Relationship to Apoptosis and to Acquired Prostatic Cell Androgen Independence1. , 1999, Endocrinology.

[3]  C. Stewart,et al.  Disruption of the mouse necdin gene results in early post-natal lethality , 1999, Nature Genetics.

[4]  Y. Combarnous,et al.  Involvement of G protein-coupled receptor kinases and arrestins in desensitization to follicle-stimulating hormone action. , 1999, Molecular endocrinology.

[5]  F. Brasseur,et al.  A new MAGE gene with ubiquitous expression does not code for known MAGE antigens recognized by T cells. , 1999, Cancer research.

[6]  J. M. Hall,et al.  Identification of a new, unorthodox member of the MAGE gene family. , 1999, Genomics.

[7]  E. Nieschlag,et al.  Role of FSH in male gonadal function. , 1999, Annales d'endocrinologie.

[8]  K. Yoshikawa,et al.  Physical and Functional Interactions of Neuronal Growth Suppressor Necdin with p53* , 1999, The Journal of Biological Chemistry.

[9]  M. Benahmed,et al.  Prolactin receptors are expressed and hormonally regulated in rat Sertoli cells , 1999, Molecular and Cellular Endocrinology.

[10]  T. Kameda,et al.  The effect of follicle-stimulating hormone (FSH) on the expression of FSH receptor in cultured rat granulosa cells. , 1999, Life sciences.

[11]  P. Chomez,et al.  A new family of mouse genes homologous to the human MAGE genes. , 1999, Genomics.

[12]  K. Arden,et al.  Identification of a new MAGE gene with tumor-specific expression by representational difference analysis. , 1998, Cancer research.

[13]  C. Henderson,et al.  Synergistic Effects of Schwann- and Muscle-Derived Factors on Motoneuron Survival Involve GDNF and Cardiotrophin-1 (CT-1) , 1998, The Journal of Neuroscience.

[14]  K. Yoshikawa,et al.  Necdin, A Postmitotic Neuron-specific Growth Suppressor, Interacts with Viral Transforming Proteins and Cellular Transcription Factor E2F1* , 1998, The Journal of Biological Chemistry.

[15]  A. Monaco,et al.  Two members of the human MAGEB gene family located in Xp21.3 are expressed in tumors of various histological origins. , 1997, Genomics.

[16]  S. Taviaux,et al.  The human necdin gene, NDN, is maternally imprinted and located in the Prader-Willi syndrome chromosomal region , 1997, Nature Genetics.

[17]  P. Durand,et al.  Pre- and postmeiotic expression of male germ cell-specific genes throughout 2-week cocultures of rat germinal and Sertoli cells. , 1997, Biology of reproduction.

[18]  N. Bunnett,et al.  Regulatory mechanisms that modulate signalling by G-protein-coupled receptors. , 1997, The Biochemical journal.

[19]  Aaron P. Campbell,et al.  Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[20]  K. Yoshikawa,et al.  Structure and Expression of the Mouse Necdin Gene , 1996, The Journal of Biological Chemistry.

[21]  K. Takagi,et al.  Arrest of cell growth by necdin, a nuclear protein expressed in postmitotic neurons. , 1995, Biochemical and biophysical research communications.

[22]  B. Sente,et al.  Androgen-independent effects of prolactin on the different lobes of the immature rat prostate , 1995, Molecular and Cellular Endocrinology.

[23]  A. Monaco,et al.  Isolation and characterization of a MAGE gene family in the Xp21.3 region. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[24]  E. P. Smith,et al.  Insulin-like growth factor (IGF) binding protein 3 in the rat testis: follicle-stimulating hormone dependence of mRNA expression and inhibition of IGF-I action on cultured Sertoli cells. , 1995, Biology of reproduction.

[25]  M. Griswold.  Interactions between germ cells and Sertoli cells in the testis. , 1995, Biology of reproduction.

[26]  W. Stetler-Stevenson,et al.  Follicle-stimulating hormone increases the expression of tissue inhibitors of metalloproteinases TIMP-1 and TIMP-2 and induces TIMP-1 AP-1 site binding complex(es) in prepubertal rat Sertoli cells. , 1994, Endocrinology.

[27]  K. Hamil,et al.  Cloning of rat Sertoli cell follicle-stimulating hormone primary response complementary deoxyribonucleic acid: regulation of TSC-22 gene expression. , 1994, Endocrinology.

[28]  P. Sassone-Corsi,et al.  Pituitary hormone FSH directs the CREM functional switch during spermatogenesis , 1993, Nature.

[29]  L. Russell,et al.  Structural manifestations of the rat Sertoli cell to hypophysectomy: a correlative morphometric and endocrine study. , 1992, Endocrinology.

[30]  B. Sente,et al.  Dose-dependent effects of human prolactin on the immature hypophysectomized rat testis. , 1992, Endocrinology.

[31]  P. Chomez,et al.  A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. , 1991, Science.

[32]  K. Yoshikawa,et al.  A novel brain-specific mRNA encoding nuclear protein (necdin) expressed in neurally differentiated embryonal carcinoma cells. , 1991, Biochemical and biophysical research communications.

[33]  M. Parmentier,et al.  Follitropin receptor down-regulation involves a cAMP-dependent post-transcriptional decrease of receptor mRNA expression , 1991, Molecular and Cellular Endocrinology.

[34]  V. Papadopoulos,et al.  Pachytene spermatocytes regulate the secretion of Sertoli cell protein(s) which stimulate Leydig cell steroidogenesis , 1991, Molecular and Cellular Endocrinology.

[35]  M. Skinner.  Cell-cell interactions in the testis. , 1991, Annals of the New York Academy of Sciences.

[36]  D. D. de Kretser,et al.  A quantitative study of spermatogenesis in the developing rat testis. , 1990, Biology of reproduction.

[37]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[38]  R. W. Wright,et al.  Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions. , 1986, Analytical biochemistry.

[39]  Y. Combarnous,et al.  Rapid in vitro desensitization of the testosterone response in rat Ley dig cells by sub‐active concentrations of porcine luteinizing hormone , 1985, FEBS letters.

[40]  G. Verhoeven,et al.  A factor in spent media from Sertoli cell-enriched cultures that stimulates steroidogenesis in Leydig cells , 1985, Molecular and Cellular Endocrinology.

[41]  P. Lowry,et al.  Hydrophobic-interaction chromatography and anion-exchange chromatography in the presence of acetonitrile. A two-step purification method for human prolactin. , 1981, The Biochemical journal.

[42]  G. Hennen,et al.  Porcine follitropin. Isolation and characterization of the native hormone and its alpha and beta subunits. , 1978, European journal of biochemistry.

[43]  G. Hennen,et al.  Human luteinizing hormone. Isolation and characterization of the native hormone and its alpha and beta subunits. , 1975, European journal of biochemistry.

[44]  M. Welsh,et al.  Rat sertoli cells: a rapid method for obtaining viable cells. , 1975, Endocrinology.

[45]  M. Montminy,et al.  Transcriptional regulation by cyclic AMP. , 1997, Annual review of biochemistry.

[46]  G. Hennen,et al.  Biopotency of highly purified porcine FSH and human LH on gonadal function. , 1989, The Journal of endocrinology.