Molecular characterization of Staphylococcus spp. isolated from respiratory tract of apparently healthy and clinically sick sheep and goat in Nagpur, India

Present study was undertaken to characterize Staphylococcus spp. associated with respiratory tract infection in small ruminants. A total of 139 bacterial isolates were recovered from clinically sick and apparently healthy small ruminants. The prevalence rate of Staphylococcus spp. in sick and healthy animals was 44.94 and 24% respectively. The sensitivity pattern of selected antibiotics was similar in both clinically sick and apparently healthy animals except streptomycin and penicillin-G. PCR assay was standardized for the detection of pathogenic genes of Staphylococcus spp. viz. clfA and spa. Among 34 random isolates selected, the prevalence rate of clfA and spa gene was 79.41 and 58.82% respectively indicating their pathogenic potential. In conclusion, Staphylococcus spp. was found to be one of the highly prevalent organism in respiratory tract infections.

[1]  Asaduzzaman,et al.  Isolation and identification of bacteria from upper respiratory tract of Black Bengal goat in Bangladesh and investigation of some epidemiological parameters related to pneumonia , 2013 .

[2]  M. Yesuf,et al.  Histopathologicaland Bacteriological Examination of Pneumonic Lungs of Small Ruminants Slaughtered at Gondar, Ethiopia , 2012 .

[3]  Molecular Detection of Virulence Genes Associated with Pathogenicity of Klebsiella spp . Isolated from the Respiratory Tract of Apparently Healthy as well as Sick Goats , 2012 .

[4]  M. Açık,et al.  Investigation of virulence genes by PCR in Stapylococcus aureus isolates originated from subclinical bovine mastitis in Turkey. , 2011 .

[5]  E. Rahimi,et al.  Detection of some virulence factors in Staphylococcus aureus isolated from clinical and subclinical bovine mastitis in Iran , 2010 .

[6]  D. Lacasta,et al.  Influence of climatic factors on the development of pneumonia in lambs , 2008 .

[7]  T. Sisay,et al.  The Aerobic Bacterial flora of the Respiratory passageways of healthy goats in Dire Dawa Abattoir, Eastern Ethiopia , 2006 .

[8]  L. Potgieter,et al.  Ovine progressive pneumonia (Maedi-Visna): an emerging respiratory disease of sheep in Ethiopia. , 2002, DTW. Deutsche tierarztliche Wochenschrift.

[9]  K. Wilson.  Preparation of Genomic DNA from Bacteria , 2001, Current protocols in molecular biology.

[10]  R. Kumar.  Preparation of genomic DNA from bacteria , 2001 .

[11]  W. B. Martin.  Respiratory infections of sheep. , 1996, Comparative immunology, microbiology and infectious diseases.

[12]  B. Markey.  Clinical Veterinary Microbiology , 1993 .

[13]  F. O'grady.  Manual for the Identification of Medical Bacteria , 1966 .

[14]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists.

[15]  S. T. Cowan,et al.  Manual for the identification of medical bacteria. , 1960 .