The mTOR Pathway Is Activated in Human Autosomal-Recessive Polycystic Kidney Disease

Background: An inappropriate activation of the mTOR pathway was demonstrated in the autosomal dominant (AD) form of polycystic kidney disease (PKD). To date it is unclear whether the mTOR pathway is activated in autosomal-recessive (AR) PKD, a cystic disease which occurs in childhood. The purpose of the present study was to evaluate the mTOR pathway in AR PKD. Methods: We evaluated the expression of mTOR pathway molecules in paraffin-embedded liver and kidney samples from patients with AR PKD and control specimens from animals as well as humans. Monoclonal antibodies, the phosphorylated proteins pmTOR, pS6-ribosomal-protein (pS6K), p4E-BP1, peIF4G, and phospho-tuberin/TSC2 were used. Results: mTOR was strongly expressed in renal cyst-lining cells and bile ducts from AR PKD specimen. S6K immunostaining was strong in smaller tubules and weak both in larger renal cysts and in the bile duct epithelium. In controls, mTOR and S6K were expressed in distal tubule segments. 4E-BP1-immunostaining was restricted to noncystic tubules in AR PKD. eIFG4-immunostaining was observed in bile duct epithelium in AR PKD, but not in control tissue. Tuberin/TSC2 immunostaining was negative in all specimens. Conclusion: Our data suggest that the mTOR pathway may be activated in AR PKD, and mTOR molecules may represent a potential target to slow down cyst development in this disease.

[1]  B. Rudolph,et al.  Activation of the AKT/mTOR pathway in autosomal recessive polycystic kidney disease (ARPKD). , 2009, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[2]  C. Edelstein Mammalian target of rapamycin and caspase inhibitors in polycystic kidney disease. , 2008, Clinical journal of the American Society of Nephrology : CJASN.

[3]  N. LaRusso,et al.  A mouse model of autosomal recessive polycystic kidney disease with biliary duct and proximal tubule dilatation. , 2007, Kidney international.

[4]  P. Wahl,et al.  Mitotic activation of Akt signalling pathway in Han:SPRD rats with polycystic kidney disease , 2007, Nephrology.

[5]  Ming Wu,et al.  Everolimus Retards Cyst Growth and Preserves Kidney Function in a Rodent Model for Polycystic Kidney Disease , 2007, Kidney and Blood Pressure Research.

[6]  P. Harris,et al.  Genotype-phenotype correlations in autosomal dominant and autosomal recessive polycystic kidney disease. , 2007, Journal of the American Society of Nephrology : JASN.

[7]  G. Walz Therapeutic approaches in autosomal dominant polycystic kidney disease (ADPKD): is there light at the end of the tunnel? , 2006, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[8]  A. Novick,et al.  The mTOR pathway is regulated by polycystin-1, and its inhibition reverses renal cystogenesis in polycystic kidney disease. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[9]  P. Wahl,et al.  Inhibition of mTOR with sirolimus slows disease progression in Han:SPRD rats with autosomal dominant polycystic kidney disease (ADPKD). , 2006, Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.

[10]  M. Hall,et al.  TOR Signaling in Growth and Metabolism , 2006, Cell.

[11]  Y. Tao,et al.  Rapamycin markedly slows disease progression in a rat model of polycystic kidney disease. , 2004, Journal of the American Society of Nephrology : JASN.

[12]  V. Gattone,et al.  Renal cysts of inv/inv mice resemble early infantile nephronophthisis. , 2004, Journal of the American Society of Nephrology : JASN.

[13]  Carsten Bergmann,et al.  PKHD1 mutations in families requesting prenatal diagnosis for autosomal recessive polycystic kidney disease (ARPKD) , 2004, Human mutation.

[14]  L. Aicher,et al.  Activated mammalian target of rapamycin pathway in the pathogenesis of tuberous sclerosis complex renal tumors. , 2002, Cancer research.

[15]  A. Kribben,et al.  Differential tissue distribution of the Invs gene product inversin , 2005, Cell and Tissue Research.

[16]  J. García Rodríguez,et al.  [Polycystic Kidney Disease]. , 2005, Actas urologicas espanolas.

[17]  Kun-Liang Guan,et al.  Dysregulation of the TSC-mTOR pathway in human disease , 2004, Nature Genetics.

[18]  J. Avruch,et al.  The p70 S6 kinase integrates nutrient and growth signals to control translational capacity. , 2001, Progress in molecular and subcellular biology.

[19]  V. Torres,et al.  The pck rat: a new model that resembles human autosomal dominant polycystic kidney and liver disease. , 2001, Kidney international.