Functionalized Silver Nano-Sensor for Colorimetric Detection of Hg2+ Ions: Facile Synthesis and Docking Studies

In the present study, we describe the facile synthesis of silver nanoparticles (AgNPs) and their nanostructures functionalized with 2-aminopyrimidine-4,6-diol (APD-AgNPs) for Hg2+ ion detection. The promising colorimetric response of APD-AgNPs to detect Hg2+ ions was visible with naked eyes and spectroscopic changes were examined by using a UV-Visible spectrophotometer. The aggregation of APD-AgNPs upon addition of Hg2+ ions was due to the chelation effect of the functionalized nanostructures and results in a color change from pale brown to deep yellow color. The probing sensitivity was observed within five minutes with a detection limit of about 0.35 µM/L. The TEM images of APD-AgNPs showed polydispersed morphologies with hexagonal, heptagonal and spherical nanostructures with an average size between 10 to 40 nm. Furthermore, the sensing behavior of APD-AgNPs towards Hg2+ ions detection was investigated using docking and interaction studies.

[1]  Chad A Mirkin,et al.  Silver nanoparticle-oligonucleotide conjugates based on DNA with triple cyclic disulfide moieties. , 2007, Nano letters.

[2]  D. R. Shankaran,et al.  Functionalized silver nanoparticles probe for visual colorimetric sensing of mercury , 2016 .

[3]  Zhenyu Lin,et al.  Ultrasensitive and selective electrochemical biosensor for detection of mercury (II) ions by nicking endonuclease-assisted target recycling and hybridization chain reaction signal amplification. , 2017, Biosensors & bioelectronics.

[4]  D. Adriano.  Lead, Mercury, Cadmium and Arsenic in the Environment , 1988 .

[5]  S. P. Anthony,et al.  Green synthesized silver nanoparticles for selective colorimetric sensing of Hg2+ in aqueous solution at wide pH range. , 2013, The Analyst.

[6]  X. Hou,et al.  Direct detection of mercury in vapor and aerosol from chemical atomization and nebulization at ambient temperature: exploiting the flame atomic absorption spectrometer , 2005 .

[7]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[8]  Y. Fujii,et al.  Selective Determination of Mercury(II) Ion in Water by Solvent Extraction Followed by Reversed‐Phase HPLC , 2004 .

[9]  Gadadhar Barman,et al.  Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles , 2016, Applied Nanoscience.

[10]  Ping Wang,et al.  A miniaturized electrochemical system for high sensitive determination of chromium(VI) by screen-printed carbon electrode with gold nanoparticles modification , 2018, Sensors and Actuators B: Chemical.

[11]  D. Reinhoudt,et al.  Development of a disposable mercury ion-selective optode based on trityl-picolinamide as ionophore. , 2007, Analytica chimica acta.

[12]  Thomas P. Moyer,et al.  The determination of mercury in whole blood and urine by inductively coupled plasma mass spectrometry , 1999 .

[13]  Y. Li,et al.  A sensitive resonance light scattering spectrometry of trace Hg2+ with sulfur ion modified gold nanoparticles. , 2009, Analytica chimica acta.

[14]  Takashi Sasaki,et al.  Recent progress on nanovehicles. , 2006, Chemical Society reviews.

[15]  Fan Yang,et al.  Colorimetric biosensing of mercury(II) ion using unmodified gold nanoparticle probes and thrombin-binding aptamer. , 2010, Biosensors & bioelectronics.

[16]  V. Vinod Kumar,et al.  Bio-functionalized silver nanoparticles for selective colorimetric sensing of toxic metal ions and antimicrobial studies. , 2014, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[17]  M. Annadhasan,et al.  Green Synthesized Silver and Gold Nanoparticles for Colorimetric Detection of Hg2+, Pb2+, and Mn2+ in Aqueous Medium , 2014 .

[18]  Weiwei Wang,et al.  Facile colorimetric detection of Hg2+ based on anti-aggregation of silver nanoparticles. , 2014, Biosensors & bioelectronics.

[19]  D. R. Bae,et al.  Lysine-functionalized silver nanoparticles for visual detection and separation of histidine and histidine-tagged proteins. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[20]  Juan Li,et al.  Highly selective, colorimetric detection of Hg2+ based on three color changes of AuNPs solution from red through sandy beige to celandine green , 2017 .

[21]  I. Leray,et al.  Design principles of fluorescent molecular sensors for cation recognition , 2000 .

[22]  Zhengbo Chen,et al.  Colorimetric Detection of Hg2+ Based on the Growth of Aptamer-Coated AuNPs: The Effect of Prolonging Aptamer Strands. , 2017, Small.

[23]  Dylan W Domaille,et al.  Metals in neurobiology: probing their chemistry and biology with molecular imaging. , 2008, Chemical reviews.

[24]  Li Li,et al.  Label-free aptamer-based colorimetric detection of mercury ions in aqueous media using unmodified gold nanoparticles as colorimetric probe , 2009, Analytical and bioanalytical chemistry.

[25]  Huan‐Tsung Chang,et al.  Detection of mercury(II) ions using colorimetric gold nanoparticles on paper-based analytical devices. , 2014, Analytical chemistry.

[26]  Elizabeth M. Nolan,et al.  Tools and tactics for the optical detection of mercuric ion. , 2008, Chemical reviews.

[27]  G. Shruthi,et al.  Green Synthesis of Biologically Active Silver Nanoparticles through a Phyto-Mediated Approach Using Areca catechu Leaf Extract , 2017 .

[28]  Ping Wang,et al.  Screen-printed gold electrode with gold nanoparticles modification for simultaneous electrochemical determination of lead and copper , 2015 .