Stabilization of α-chymotrypsin at air-water interface through surface binding to gold nanoparticle scaffolds.

Gold nanoparticles stabilize chymotrypsin (ChT) against denaturation at the air-water interface through catenation and preferential localization of the nanoparticles at the air-water interface with concomitant decrease in interfacial energy.

[1]  C. Radke,et al.  Protein adsorption at the oil/water interface: characterization of adsorption kinetics by dynamic interfacial tension measurements. , 1999, Biophysical chemistry.

[2]  W. Kegel,et al.  Spontaneous assembly of a monolayer of charged gold nanocrystals at the water/oil interface. , 2004, Angewandte Chemie.

[3]  T. Emrick,et al.  Mixed monolayer coverage on gold nanoparticles for interfacial stabilization of immiscible fluids. , 2005, Chemical communications.

[4]  V. Rotello,et al.  Highly Efficient Biocatalysts via Covalent Immobilization of Candida rugosa Lipase on Ethylene Glycol‐Modified Gold–Silica Nanocomposites , 2004 .

[5]  A. Kiener,et al.  Industrial biocatalysis today and tomorrow , 2001, Nature.

[6]  H. Gies,et al.  Protein encapsulation in mesoporous silicate: the effects of confinement on protein stability, hydration, and volumetric properties. , 2004, Journal of the American Chemical Society.

[7]  Todd Emrick,et al.  Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds. , 2004, Journal of the American Chemical Society.

[8]  S. N. Timasheff,et al.  On the role of surface tension in the stabilization of globular proteins , 1996, Protein science : a publication of the Protein Society.

[9]  K. Kjaer,et al.  Adaptive chemistry of bifunctional gold nanoparticles at the air/water interface. A synchrotron X-ray study of giant amphiphiles. , 2004, Faraday discussions.

[10]  M. Hartmann.  Ordered Mesoporous Materials for Bioadsorption and Biocatalysis , 2005 .

[11]  Peter A. Williams,et al.  Inhibition of Protein Adsorption onto Silica by Polyvinylpyrrolidone , 2002 .

[12]  L. Brinchi,et al.  Activation and stabilization of α‐chymotrypsin by cationic additives , 2001 .

[13]  M. Kantam,et al.  Highly Efficient “Tight Fit” Immobilization of α‐Chymotrypsin in Mesoporous MCM‐41: A Novel Approach Using Precursor Immobilization and Activation , 2003, Biotechnology progress.

[14]  Gary M Nishioka,et al.  Protein damage in drop-on-demand printers. , 2004, Journal of the American Chemical Society.

[15]  Lu-Yun Lian,et al.  A simple method for improving protein solubility and long-term stability. , 2004, Journal of the American Chemical Society.

[16]  S J Prestrelski,et al.  Factors affecting short-term and long-term stabilities of proteins. , 2001, Advanced drug delivery reviews.

[17]  D. Shah,et al.  Stabilization of High Ionic Strength Slurries Using Surfactant Mixtures: Molecular Factors That Determine Optimal Stability , 2002 .

[18]  Itamar Willner,et al.  Integrated nanoparticle-biomolecule hybrid systems: synthesis, properties, and applications. , 2004, Angewandte Chemie.

[19]  H. Möhwald,et al.  Directing self-assembly of nanoparticles at water/oil interfaces. , 2004, Angewandte Chemie.

[20]  C. Kumar,et al.  Efficient Renaturation of Immobilized Met-hemoglobin at the Galleries of α-Zirconium Phosphonate , 2001 .

[21]  G. Whitesides,et al.  Self-assembled monolayers of thiolates on metals as a form of nanotechnology. , 2005, Chemical reviews.

[22]  Uwe T Bornscheuer,et al.  Immobilizing enzymes: how to create more suitable biocatalysts. , 2003, Angewandte Chemie.

[23]  T. Emrick,et al.  Nanoparticle Assembly and Transport at Liquid-Liquid Interfaces , 2003, Science.

[24]  B. Kaptein,et al.  Synthesis of a Stable Helical Peptide and Grafting on Gold Nanoparticles , 2003 .

[25]  F. Alfani,et al.  Effect of quaternary ammonium salts on the hydrolysis of N-glutaryl-l-phenylalanine catalysed by α-chymotrypsin , 2004 .

[26]  Ping Wang,et al.  Polymer-enzyme conjugates can self-assemble at oil/water interfaces and effect interfacial biotransformations. , 2004, Journal of the American Chemical Society.

[27]  V. Rotello,et al.  Monolayer-controlled substrate selectivity using noncovalent enzyme-nanoparticle conjugates. , 2004, Journal of the American Chemical Society.

[28]  F. Bordusa.  Proteases in organic synthesis. , 2002, Chemical reviews.

[29]  Christof M Niemeyer,et al.  "Belt and braces": a peptide-based linker system of de novo design. , 2003, Journal of the American Chemical Society.