Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India

The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clonal complexes. MLST analysis showed 24 new sequence types (ST) among S. haemolyticus isolates, irrespective of sources and place of isolation. The finding of this study allowed to set up an MLST database on the PubMLST.org website using BIGSdb software and made available at http://pubmlst.org/shaemolyticus/. The data of this study thus suggest that MLST can be used to study population structure and diversity among S. haemolyticus isolates.

[1]  K. Hiramatsu,et al.  Rapid and Accurate Identification of Human-Associated Staphylococci by Use of Multiplex PCR , 2011, Journal of Clinical Microbiology.

[2]  G. Mehta,et al.  Multi-resistant Staphylococcus haemolyticus in a neonatal unit in New Delhi. , 1997, Annals of tropical paediatrics.

[3]  A. Ankai,et al.  Whole-Genome Sequencing of Staphylococcus haemolyticus Uncovers the Extreme Plasticity of Its Genome and the Evolution of Human-Colonizing Staphylococcal Species , 2005, Journal of bacteriology.

[4]  Sasmita Panda,et al.  Development and evaluation of hexaplex PCR for rapid detection of methicillin, cadmium/zinc and antiseptic-resistant staphylococci, with simultaneous identification of PVL-positive and -negative Staphylococcus aureus and coagulase negative staphylococci. , 2014, FEMS microbiology letters.

[5]  P. François,et al.  Core genome conservation of Staphylococcus haemolyticus limits sequence based population structure analysis. , 2012, Journal of microbiological methods.

[6]  R. Hirata,et al.  Staphylococcus haemolyticus disseminated among neonates with bacteremia in a neonatal intensive care unit in Rio de Janeiro, Brazil. , 2014, Diagnostic microbiology and infectious disease.

[7]  M. Giambiagi-deMarval,et al.  Staphylococcus haemolyticus as an Important Hospital Pathogen and Carrier of Methicillin Resistance Genes , 2011, Journal of Clinical Microbiology.

[8]  Phenotypic and genotypic characterization of coagulase negative staphylococci (CoNS) other than Staphylococcus epidermidis isolated from ocular infections. , 2011, Investigative ophthalmology & visual science.

[9]  J. Froggatt,et al.  Antimicrobial resistance in nosocomial isolates of Staphylococcus haemolyticus , 1989, Antimicrobial Agents and Chemotherapy.

[10]  Martin C. J. Maiden,et al.  BIGSdb: Scalable analysis of bacterial genome variation at the population level , 2010, BMC Bioinformatics.