IMMUNOMAGNETIC REDUCTION FOR FETAL FIBRONECTIN DETECTION | A NOVEL METHOD TO DETECT THE PRETERM BIOMARKER

Preterm birth is the main cause of perinatal morbidity and mortality throughout the world. Fetal fibronectin (fFN) is one type of glycoprotein detected in the interface of the choriodecidual junction. Preterm labor is related to the disruption of the choriodecidual junction and thus fFN releases in the ectocervix or posterior vaginal fornix. Quantization of fFN can assist the diagnosis and prevention of preterm birth. In this work, we developed an immunoassay, the immunomagnetic reduction (IMR), to quantitatively detect fFN. It was found that the low detection limit for fFN via IMR is less than 10 ng/ml, which is much lower than clinic criteria 50 ng/ml. In addition to the high sensitivity, IMR assay shows such merits as low cost and high reliability for detecting fFN.

[1]  G. Lockitch,et al.  Fetal fibronectin improves the accuracy of diagnosis of preterm labor. , 1995, American journal of obstetrics and gynecology.

[2]  Shieh-Yueh Yang,et al.  Hyper-high-sensitivity wash-free magnetoreduction assay on biomolecules using high- Tc superconducting quantum interference devices , 2008 .

[3]  John Clarke,et al.  Magnetic gradiometer based on a high-transition temperature superconducting quantum interference device for improved sensitivity of a biosensor , 2002 .

[4]  K. Titani,et al.  The oncofetal structure of human fibronectin defined by monoclonal antibody FDC-6. Unique structural requirement for the antigenic specificity provided by a glycosylhexapeptide. , 1988, The Journal of biological chemistry.

[5]  M. Dombrowski,et al.  The preterm prediction study: quantitative fetal fibronectin values and the prediction of spontaneous preterm birth. The National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network. , 2000, American journal of obstetrics and gynecology.

[6]  M. Dombrowski,et al.  The Preterm Prediction Study: association between cervical interleukin 6 concentration and spontaneous preterm birth. National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network. , 2001, American journal of obstetrics and gynecology.

[7]  C. Gale,et al.  Prevention of age related macular degeneration , 2002, BMJ : British Medical Journal.

[8]  M MAYER,et al.  [The prevention of premature birth]. , 1953, La semaine des hopitaux : organe fonde par l'Association d'enseignement medical des hopitaux de Paris.

[9]  T. Garite,et al.  Fetal fibronectin detection as a predictor of preterm birth in actual clinical practice. , 2000, American journal of obstetrics and gynecology.

[10]  R. H. Holbrook,et al.  Fetal fibronectin as a predictor of preterm birth in patients with symptoms: a multicenter trial. , 1997, American journal of obstetrics and gynecology.

[11]  Bo Jacobsson,et al.  Monocyte chemotactic protein-1 in cervical and amniotic fluid: relationship to microbial invasion of the amniotic cavity, intra-amniotic inflammation, and preterm delivery. , 2003, American journal of obstetrics and gynecology.

[12]  Keiji Enpuku,et al.  Detection of Magnetic Nanoparticles with Superconducting Quantum Interference Device (SQUID) Magnetometer and Application to Immunoassays , 1999 .

[13]  U. Nicolini,et al.  Cervical phIGFBP‐1 in the evaluation of the risk of preterm delivery , 2007, Acta obstetricia et gynecologica Scandinavica.

[14]  F. Arechavaleta-Velasco,et al.  Vaginal Matrix Metalloproteinase Levels in Pregnant Women With Bacterial Vaginosis , 2006, The Journal of the Society for Gynecologic Investigation: JSGI.

[15]  Chin-Yih Hong,et al.  Wash-free, antibody-assisted magnetoreduction assays of orchid viruses. , 2008, Journal of virological methods.

[16]  H. C. Yang,et al.  Biofunctionalized magnetic nanoparticles for high-sensitivity immunomagnetic detection of human C-reactive protein , 2006 .

[17]  R. Goldenberg,et al.  Biochemical markers for the prediction of preterm birth. , 2005, American journal of obstetrics and gynecology.

[18]  Philip Steer,et al.  The epidemiology of preterm labor – a global perspective , 2005, Journal of perinatal medicine.

[19]  R. Goldenberg,et al.  Vaginal Fetal Fibronectin Levels and Spontaneous Preterm Birth in Symptomatic Women , 2001, Obstetrics and gynecology.

[20]  S. Hakomori,et al.  The oncofetal domain of fibronectin defined by monoclonal antibody FDC-6: its presence in fibronectins from fetal and tumor tissues and its absence in those from normal adult tissues and plasma. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J K Todd,et al.  Bacterial protease-induced reduction of chorioamniotic membrane strength and elasticity. , 1987, Obstetrics and gynecology.

[22]  Shieh-Yueh Yang,et al.  Magnetic susceptibility reduction method for magnetically labeled immunoassay , 2006 .

[23]  R L Berkowitz,et al.  The presence of cervical and vaginal fetal fibronectin predicts preterm delivery in an inner-city obstetric population. , 1993, American journal of obstetrics and gynecology.

[24]  Roberto Romero,et al.  Epidemiology and causes of preterm birth , 2008, The Lancet.

[25]  Lutz Trahms,et al.  Determination of the binding reaction between avidin and biotin by relaxation measurements of magnetic nanoparticles , 1999 .

[26]  Shieh-Yueh Yang,et al.  Magnetic relaxation measurement in immunoassay using high-transition-temperature superconducting quantum interference device system , 2006 .

[27]  C. Y. Yang,et al.  Magnetically enhanced high-specificity virus detection using bio-activated magnetic nanoparticles with antibodies as labeling markers. , 2010, Journal of virological methods.

[28]  D. Rouse,et al.  Prevention of premature birth. , 1998, The New England journal of medicine.

[29]  D. Ingbar,et al.  An in vitro model for polymorphonuclear-leukocyte-induced injury to an extracellular matrix. Relative contribution of oxidants and elastase to fibronectin release from amnionic membranes. , 1986, The American review of respiratory disease.

[30]  Lucas M Bachmann,et al.  Accuracy of cervicovaginal fetal fibronectin test in predicting risk of spontaneous preterm birth: systematic review , 2002, BMJ : British Medical Journal.

[31]  A. Fanaroff,et al.  Outcomes of children of extremely low birthweight and gestational age in the 1990's. , 1999, Early human development.