Some Effects of Electrical Fields on Red Blood Cells with Remarks on Electronic Red Cell Sizing

Sizing normal RBC by an electronic method showed that with certain aperture currents a skewed bimodal size‐distribution curve is obtainable. In these studies some possible causes for such distributions were investigated. The experiments show that an electrical field can cause change in shape, size, and staining properties of RBC as well as lysis. Extrapolation of the results of these experiments to the conditions of the RBC sizing method indicate that these changes are not responsible for the skewed bimodal distribution. We believe, therefore, that normal RBC vary among themselves in some structural or physiologic way, not necessarily size, that was measured by this system.

[1]  R I Weed,et al.  The influence of hemoglobin concentration on the distribution pattern of the volumes of human erythrocytes. , 1967, Blood.

[2]  E. C. Gregg,et al.  Electrical counting and sizing of mammalian cells in suspension. , 1965, Biophysical journal.

[3]  H Winter,et al.  The skewness of volume distribution curves of erythrocytes. , 1965, The Australian journal of experimental biology and medical science.

[4]  Lushbaugh Cc,et al.  Rapid electronic red blood cell sizing as an aid in clinical diagnosis. , 1965 .

[5]  C C LUSHBAUGH,et al.  Electronic measurement of cellular volumes. II. Frequency distribution of erythrocyte volumes. , 1961, LA [reports]. U.S. Atomic Energy Commission.

[6]  F. S. Brackett,et al.  Determination of number and size of particles by electrical gating: blood cells. , 1957, Journal of applied physiology.

[7]  G. Brecher,et al.  SIZE DISTRIBUTION OF ERYTHROCYTES , 1962, Annals of the New York Academy of Sciences.

[8]  J. GUTMANN.  Elektronische Verfahren zur Ermittlung statistischer Maßzahlen einiger medizinisch wichtiger Daten , 1966 .

[9]  C C LUSHBAUGH,et al.  Electronic measurement of cellular volumes. I. Calibration of the apparatus. , 1961, LA [reports]. U.S. Atomic Energy Commission.

[10]  T. Prankerd.  The ageing of red cells , 1958, The Journal of physiology.

[11]  A. C. Burton,et al.  Reversible Changes in Shape of Red Cells in Electrical Fields , 1965, Nature.

[12]  E. C. Gregg,et al.  Electrical counting and sizing of mammalian cells in suspension: an experimental evaluation. , 1967, Physics in medicine and biology.

[13]  R. Furchgott.  Disk-Sphere Transformation in Mammalian Red Cells , 1940 .

[14]  I. Ohad,et al.  EFFECTS OF HIGH-FREQUENCY ELECTRIC FIELDS ON THE LIVING CELL. I. BEHAVIOUR OF HUMAN ERYTHROCYTES IN HIGH-FREQUENCY ELECTRIC FIELDS AND ITS RELATION TO THEIR AGE. , 1964, Biochimica et biophysica acta.

[15]  C C Lushbaugh,et al.  Rapid Electronic Red Blood Cell Sizing as an Aid in Clinical Diagnosis , 1965, Southern medical journal.