Tissue Microarray: A rapidly evolving diagnostic and research tool

Tissue microarray is a recent innovation in the field of pathology. A microarray contains many small rep-resentative tissue samples from hundreds of different cases assembled on a single histologic slide, and therefore allows high throughput analysis of multiple specimens at the same time. Tissue microarrays are paraffin blocks produced by extracting cylindrical tissue cores from different paraffin donor blocks and re-embedding these into a single recipient (microarray) block at defined array coordinates. Using this technique, up to 1000 or more tissue samples can be arrayed into a single paraffin block. It can permit simultaneous analysis of molecular targets at the DNA, mRNA, and protein levels under identical, stan--dardized conditions on a single glass slide, and also provide maximal preservation and use of limited and irreplaceable archival tissue samples. This versatile technique, in which data analysis is automated facilitates retrospective and prospective human tissue studies. It is a practical and effective tool for high- throughput molecular analysis of tissues that is helping to identify new diagnostic and prognostic markers and targets in human cancers, and has a range of potential applications in basic research, prognostic on--cology and drug discovery. This article summarizes the technical aspects of tissue microarray construction and sectioning, advantages, application, and limitations.

[1]  G. Hostetter,et al.  Tissue microarrays: applications in genomic research , 2005, Expert review of molecular diagnostics.

[2]  Beena Kumar,et al.  Tissue microarrays: a practical guide. , 2004, Pathology.

[3]  Brian E Matysiak,et al.  Simple, Inexpensive Method for Automating Tissue Microarray Production Provides Enhanced Microarray Reproducibility , 2003, Applied immunohistochemistry & molecular morphology : AIMM.

[4]  S H Barsky,et al.  Semi‐automated imaging system to quantitate estrogen and progesterone receptor immunoreactivity in human breast cancer , 2007, Journal of microscopy.

[5]  H. Battifora.  The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. , 1986, Laboratory investigation; a journal of technical methods and pathology.

[6]  T. Yeatman,et al.  The promise of microarray technology in melanoma care. , 2002, Cancer control : journal of the Moffitt Cancer Center.

[7]  Matt van de Rijn,et al.  Tissue Microarrays Are an Effective Quality Assurance Tool for Diagnostic Immunohistochemistry , 2002, Modern Pathology.

[8]  P. Furmanski,et al.  A rapid and efficient method for testing immunohistochemical reactivity of monoclonal antibodies against multiple tissue samples simultaneously. , 1987, Journal of immunological methods.

[9]  G. Sauter,et al.  Colorectal carcinoma from Saudi Arabia. Analysis of MLH-1, MSH-2 and p53 genes by immunohistochemistry and tissue microarray analysis. , 2006, Saudi medical journal.

[10]  Marek Skacel,et al.  Tissue Microarrays: A Powerful Tool for High-Throughput Analysis of Clinical Specimens: A Review of the Method With Validation Data , 2002, Applied immunohistochemistry & molecular morphology : AIMM.

[11]  H. Moch,et al.  High-throughput tissue microarray analysis to evaluate genes uncovered by cDNA microarray screening in renal cell carcinoma. , 1999, The American journal of pathology.

[12]  D L Rimm,et al.  Tissue microarray: a new technology for amplification of tissue resources. , 2001, Cancer journal.

[13]  Jian-ying Zhang.  Mini-array of multiple tumor-associated antigens to enhance autoantibody detection for immunodiagnosis of hepatocellular carcinoma. , 2007, Autoimmunity reviews.

[14]  H. Patel,et al.  Tissue microarrays: a current medical research tool , 2004, Current medical research and opinion.

[15]  Eric Peltier,et al.  Evaluation of the interobserver reproducibility of Gleason grading of prostatic adenocarcinoma using tissue microarrays. , 2003, Human pathology.

[16]  Wei Zhang,et al.  Tissue Microarrays: Applications in Neuropathology Research, Diagnosis, and Education , 2002, Brain pathology.

[17]  S. Dhanasekaran,et al.  The polycomb group protein EZH2 is involved in progression of prostate cancer , 2002, Nature.

[18]  O. Kallioniemi,et al.  Tissue microarray technology for high-throughput molecular profiling of cancer. , 2001, Human molecular genetics.

[19]  R. Romero,et al.  Tissue microarray: An effective high-throughput method to study the placenta for clinical and research purposes , 2006, The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians.

[20]  D L Rimm,et al.  Amplification of tissue by construction of tissue microarrays. , 2001, Experimental and molecular pathology.

[21]  M. Salto‐Tellez,et al.  Reliability of Tissue Microarrays in Detecting Protein Expression and Gene Amplification in Breast Cancer , 2003, Modern Pathology.

[22]  D. Rimm,et al.  Validation of Tissue Microarray Technology in Breast Carcinoma , 2000, Laboratory Investigation.

[23]  J. Masters,et al.  21st Century molecular biology in urology , 2001, BJU international.

[24]  C Blake Gilks,et al.  Assessment of interlaboratory variation in the immunohistochemical determination of estrogen receptor status using a breast cancer tissue microarray. , 2002, American journal of clinical pathology.

[25]  B. Jasani,et al.  A formalin-fixed, paraffin-processed cell line standard for quality control of immunohistochemical assay of HER-2/neu expression in breast cancer. , 2002, American journal of clinical pathology.

[26]  Ronald Simon,et al.  Tissue microarrays for high-throughput molecular pathology , 2004, Annals of Saudi medicine.

[27]  J. Giltnane,et al.  Technology Insight: identification of biomarkers with tissue microarray technology , 2004, Nature Clinical Practice Oncology.

[28]  G. Chatta.  Multiplex biomarker approach for determining risk of prostate-specific antigen-defined recurrence of prostate cancer , 2003 .

[29]  O. Kallioniemi,et al.  Clinical and functional target validation using tissue and cell microarrays. , 2002, Current opinion in chemical biology.

[30]  Holger Moch,et al.  Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade , 2001, The Journal of pathology.

[31]  Z. Xiping,et al.  Advantages and applications of tissue microarray technology on cancer research , 2003 .

[32]  J. Kononen,et al.  Tissue microarrays for high-throughput molecular profiling of tumor specimens , 1998, Nature Medicine.

[33]  B. Zetter,et al.  Cancer: The silence of the genes , 2002, Nature.

[34]  H. Moch,et al.  Tissue microarrays for gene amplification surveys in many different tumor types. , 1999, Clinical cancer research : an official journal of the American Association for Cancer Research.

[35]  Arul M Chinnaiyan,et al.  Multiplex biomarker approach for determining risk of prostate-specific antigen-defined recurrence of prostate cancer. , 2003, Journal of the National Cancer Institute.

[36]  D. Slamon,et al.  Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins. , 2001, The American journal of pathology.

[37]  Matt van de Rijn,et al.  Identification of prognostically relevant and reproducible subsets of endometrial adenocarcinoma based on clustering analysis of immunostaining data , 2007, Modern Pathology.