Species Identification Using DNA Barcoding on Processed Panga Catfish Products in Viet Nam Revealed Important Mislabeling

Nucleotide sequences of the Cytochrome oxidase subunit I gene and Cytochrome b were analyzed for Ö10 processed fish products collected from supermarkets in Hanoi, Viet Nam. The similarity between our results and published data from the NCBI and BOLD was compared to identify species. This molecular analysis showed that the common names of only 4 of these products matched with their corresponding scientific names. The other six were mislabeled with an important mislabeling from P. hypophthalmus into P. boucourti. Although no commercial frauds were found in these mislabelled products, the correct scientific names of fish species should be labelled for the processed products as they are in supermarkets.

[1]  C. Woese,et al.  Phylogenetic structure of the prokaryotic domain: The primary kingdoms , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[2]  R. Vrijenhoek,et al.  DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. , 1994, Molecular marine biology and biotechnology.

[3]  C. Gissi,et al.  Evolutionary genomics in Metazoa: the mitochondrial DNA as a model system. , 1999, Gene.

[4]  Javier Terol,et al.  Statistical validation of the identification of tuna species: bootstrap analysis of mitochondrial DNA sequences. , 2002, Journal of agricultural and food chemistry.

[5]  Jeremy R. deWaard,et al.  Biological identifications through DNA barcodes , 2003, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[6]  P. Hebert,et al.  Identification of Birds through DNA Barcodes , 2004, PLoS biology.

[7]  R. Zatorre,et al.  Fisheries: Mislabelling of a depleted reef fish , 2004, Nature.

[8]  C. Meyer,et al.  DNA Barcoding: Error Rates Based on Comprehensive Sampling , 2005, PLoS biology.

[9]  R. Ward,et al.  DNA barcoding Australia's fish species , 2005, Philosophical Transactions of the Royal Society B: Biological Sciences.

[10]  P. Hebert,et al.  bold: The Barcode of Life Data System (http://www.barcodinglife.org) , 2007, Molecular ecology notes.

[11]  Rob Ogden,et al.  Validation of the barcoding gene COI for use in forensic genetic species identification. , 2007, Forensic science international.

[12]  N. Mandrak,et al.  Identifying Canadian Freshwater Fishes through DNA Barcodes , 2008, PloS one.

[13]  F. Santaclara,et al.  Development of a method for the genetic identification of flatfish species on the basis of mitochondrial DNA sequences. , 2008, Journal of agricultural and food chemistry.

[14]  Toward fish and seafood traceability: anchovy species determination in fish products by molecular markers and support through a public domain database. , 2008, Journal of agricultural and food chemistry.

[15]  R. Hanner,et al.  The campaign to DNA barcode all fishes, FISH-BOL. , 2009, Journal of fish biology.

[16]  P. Galli,et al.  DNA barcoding reveals fraudulent substitutions in shark seafood products: The Italian case of “palombo” (Mustelus spp.) , 2010 .

[17]  F. Marzano,et al.  Molecular barcoding reveals mislabelling of commercial fish products in Italy , 2010 .

[18]  Rosalee S. Hellberg,et al.  Advances in DNA-Based Techniques for the Detection of Seafood Species Substitution on the Commercial Market , 2011, Journal of laboratory automation.

[19]  D. Carvalho,et al.  DNA barcoding unveils a high rate of mislabeling in a commercial freshwater catfish from Brazil , 2011, Mitochondrial DNA.

[20]  W. Kress,et al.  DNA barcoding as a reliable method for the authentication of commercial seafood products. , 2012 .

[21]  Mehrdad Hajibabaei,et al.  DNA barcodes for everyday life: Routine authentication of Natural Health Products , 2012 .

[22]  M. Srinivasan,et al.  Identification and intra species delineation of ornamental silver pompano (Trachinotus blochi,Lacepede, 1801) with DNA barcodes , 2013 .

[23]  C. Shiau,et al.  Identification of four Thunnus tuna species using mitochondrial cytochrome b gene sequence and PCR-RFLP analysis , 2020, Journal of Food and Drug Analysis.