Boosting plant-part segmentation of cucumber plants by enriching incomplete 3D point clouds with spectral data

Plant scientists require high quality phenotypic datasets. Computer-vision based methods can improve the objectiveness and the accuracy of phenotypic measurements. In this paper, we focus on 3D point clouds for measuring plant architecture of cucumber plants, using spectral data and deep learning (DL). More specifically, the focus of this paper is on the segmentation of the point clouds, such that for each point it is known to which plant part (e.g. leaf or stem) it belongs. It was shown that the availability of spectral data can improve the segmentation, with the mean intersection-over-union rising from 0.90 to 0.95. Furthermore, we analysed the effect of uncertainty in the collection of ground truth data. For this purpose, we hand-labelled 264 point clouds of cucumber plants twice and show that the intra-observer variability between those two annotation sets can be as low as 0.49 for difficult classes, while it was 0.99 for the class with the least uncertainty. Adding the second set of hand-labelled data to the training of the network improved the segmentation performance slightly. Finally, we show the improved performance of a 4-class segmentation over an 8-class segmentation, emphasizing the need for a careful design of plant phenotyping experiments. The results presented in this paper contribute to further development of automated phenotyping methods for complex plant traits.

[1]  H. Bourne,et al.  Subunit interaction in cyclic AMP-dependent protein kinase of mutant lymphoma cells. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[2]  T. Sicheritz-Pontén,et al.  Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing , 2017, GigaScience.

[3]  David Reiser,et al.  3-D Imaging Systems for Agricultural Applications—A Review , 2016, Sensors.

[4]  Isabelle Goldringer,et al.  SHiNeMaS: a web tool dedicated to seed lots history, phenotyping and cultural practices , 2020, Plant Methods.

[5]  Leonidas J. Guibas,et al.  PointNet: Deep Learning on Point Sets for 3D Classification and Segmentation , 2016, 2017 IEEE Conference on Computer Vision and Pattern Recognition (CVPR).

[6]  D. Leister,et al.  Editorial: Relevance of Translational Regulation on Plant Growth and Environmental Responses , 2017, Frontiers in Plant Science.

[7]  Gert Kootstra,et al.  Validation of plant part measurements using a 3D reconstruction method suitable for high-throughput seedling phenotyping , 2015, Machine Vision and Applications.

[8]  David J. Griffiths,et al.  SynthCity: A large scale synthetic point cloud , 2019, ArXiv.

[9]  Jitendra Kumar,et al.  Phenomics in Crop Plants: Trends, Options and Limitations , 2015, Springer India.

[10]  Andreas Kamilaris,et al.  Deep learning in agriculture: A survey , 2018, Comput. Electron. Agric..

[11]  Gert Kootstra,et al.  Plant-part segmentation using deep learning and multi-view vision , 2019, Biosystems Engineering.

[12]  Rodomiro Ortiz,et al.  Editorial: Plant Phenotyping and Phenomics for Plant Breeding , 2017, Front. Plant Sci..

[13]  Gilles Galopin,et al.  ROSE-X: an annotated data set for evaluation of 3D plant organ segmentation methods , 2020, Plant Methods.

[14]  S. Omholt,et al.  Phenomics: the next challenge , 2010, Nature Reviews Genetics.

[15]  Christian Germain,et al.  Comparison of SIFT Encoded and Deep Learning Features for the Classification and Detection of Esca Disease in Bordeaux Vineyards , 2018, Remote. Sens..

[16]  Jose A. Jiménez-Berni,et al.  Review: New sensors and data-driven approaches—A path to next generation phenomics☆ , 2019, Plant science : an international journal of experimental plant biology.

[17]  Cris Kuhlemeier,et al.  Plant architecture , 2002, EMBO reports.

[18]  Ian Stavness,et al.  Deep Plant Phenomics: A Deep Learning Platform for Complex Plant Phenotyping Tasks , 2017, Front. Plant Sci..

[19]  Tony P. Pridmore,et al.  Deep machine learning provides state-of-the-art performance in image-based plant phenotyping , 2016, bioRxiv.

[20]  Radu Bogdan Rusu,et al.  3D is here: Point Cloud Library (PCL) , 2011, 2011 IEEE International Conference on Robotics and Automation.

[21]  David Rousseau,et al.  Segmentation of structural parts of rosebush plants with 3D point-based deep learning methods , 2020, ArXiv.

[22]  Gert Kootstra,et al.  Robust node detection and tracking in fruit-vegetable crops using deep learning and multi-view imaging , 2020 .

[23]  Leonidas J. Guibas,et al.  PointNet++: Deep Hierarchical Feature Learning on Point Sets in a Metric Space , 2017, NIPS.

[24]  Hanno Scharr,et al.  Image Analysis: The New Bottleneck in Plant Phenotyping [Applications Corner] , 2015, IEEE Signal Processing Magazine.

[25]  Marc Pollefeys,et al.  Multi-Label Semantic 3D Reconstruction Using Voxel Blocks , 2016, 2016 Fourth International Conference on 3D Vision (3DV).

[26]  Elizabeth A. Kellogg,et al.  High-throughput phenotyping. , 2017, American journal of botany.

[27]  Kristian Kersting,et al.  Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range , 2019, Remote. Sens..