Phenosafranin-Based Colorimetric-Sensing Platform for Nitrite Detection Enabled by Griess Assay

A facile and effective colorimetric-sensing platform based on the diazotization of phenosafranin for the detection of NO2− under acidic conditions using the Griess assay is presented. Diazotization of commercial phenosafranin produces a color change from purplish to blue, which enables colorimetric quantitative detection of NO2−. Optimal detection conditions were obtained at a phenosafranin concentration of 0.25 mM, HCl concentration of 0.4 M, and reaction time of 20 min. Under the optimized detection conditions, an excellent linearity range from 0 to 20 μM was obtained with a detection limit of 0.22 μM. Favorable reproducibility and selectivity of the colorimetric sensing platform toward NO2− were also verified. In addition, testing spiked ham sausage, bacon, and sprouts samples demonstrated its excellent practicability. The presented colorimetric sensing platform is a promising candidate for the detection of NO2− in real applications.

[1]  Xinglian Xu,et al.  Influence of various cooking methods on the concentrations of volatile N-nitrosamines and biogenic amines in dry-cured sausages. , 2012, Journal of food science.

[2]  C. Meininger,et al.  Rapid determination of nitrite by reversed-phase high-performance liquid chromatography with fluorescence detection. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[3]  S. Helmke,et al.  Measurement of the NO metabolites, nitrite and nitrate, in human biological fluids by GC-MS. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[4]  Baikun Li,et al.  High-temperature annealing enabled iridium oxide nanofibers for both non-enzymatic glucose and solid-state pH sensing , 2018 .

[5]  K. Hendricks,et al.  Dietary Nitrites and Nitrates, Nitrosatable Drugs, and Neural Tube Defects , 2004, Epidemiology.

[6]  J. Sebranek,et al.  Cured meat products without direct addition of nitrate or nitrite: what are the issues? , 2007, Meat science.

[7]  Lun Wang,et al.  One-pot preparation of Au-RGO/PDDA nanocomposites and their application for nitrite sensing , 2015 .

[8]  R. Compton,et al.  Detection and determination of nitrate and nitrite: a review. , 2001, Talanta.

[9]  F. Cao,et al.  Dual functional rhodium oxide nanocorals enabled sensor for both non-enzymatic glucose and solid-state pH sensing. , 2018, Biosensors & bioelectronics.

[10]  R. Compton,et al.  The use of nanoparticles in electroanalysis: a review , 2006, Analytical and bioanalytical chemistry.

[11]  Chad A. Mirkin,et al.  Colorimetric nitrite and nitrate detection with gold nanoparticle probes and kinetic end points. , 2009, Journal of the American Chemical Society.

[12]  A. Afkhami,et al.  Spectrophotometric Determination of Nitrite Based on Its Reaction with p-Nitroaniline in the Presence of Diphenylamine in Micellar Media , 2004 .

[13]  M. Amini,et al.  A highly selective optical sensor for catalytic determination of ultra-trace amounts of nitrite in water and foods based on brilliant cresyl blue as a sensing reagent , 2010 .

[14]  T. Korenaga,et al.  Ion chromatographic method for simultaneous determination of nitrate and nitrite in human saliva. , 2000, Journal of chromatography. B, Biomedical sciences and applications.

[15]  Z. Moldovan.  Kinetic Spectrophotometric Determination of Nitrite with Tropaeolin 00-Bromate System , 2010 .

[16]  E. Fernandes,et al.  Use of Fluorescence Probes for Detection of Reactive Nitrogen Species: A Review , 2006, Journal of Fluorescence.

[17]  Qin Xu,et al.  Electrochemical determination of nitrite based on poly(amidoamine) dendrimer-modified carbon nanotubes for nitrite oxidation , 2009 .

[18]  M. Hepel,et al.  Transient conformation changes of albumin adsorbed on gold piezoelectrodes , 2005 .

[19]  D. Hwang,et al.  Changes in nitrate and nitrite content of four vegetables during storage at refrigerated and ambient temperatures , 2004, Food additives and contaminants.

[20]  V. Jedlicková,et al.  Determination of nitrate and nitrite by high-performance liquid chromatography in human plasma. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[21]  S. N. Tajuddin,et al.  Acrylic microspheres-based optosensor for visual detection of nitrite. , 2016, Food chemistry.

[22]  D. Ramaiah,et al.  Efficient reaction based colorimetric probe for sensitive detection, quantification, and on-site analysis of nitrite ions in natural water resources. , 2013, Analytical chemistry.

[23]  A. Kayacier,et al.  The effects of nitrite on the survival of Clostridium sporogenes and the autoxidation properties of the Kavurma. , 2006, Meat science.

[24]  K. Kudo,et al.  Simultaneous determination of nitrate and nitrite in human plasma by gas chromatography-mass spectrometry. , 2002, Journal of analytical toxicology.

[25]  Chun‐Yuen Wong,et al.  Rapid visual and spectrophotometric nitrite detection by cyclometalated ruthenium complex. , 2017, Analytica chimica acta.

[26]  L. Skibsted,et al.  Heme-iron in lipid oxidation , 2005 .

[27]  Hui Li,et al.  Simultaneous Electrochemical Determination of Sulphite and Nitrite by a Gold Nanoparticle/Graphene-Chitosan Modified Electrode , 2013 .

[28]  Lili He,et al.  Highly sensitive and selective detection of nitrite ions using Fe3O4@SiO2/Au magnetic nanoparticles by surface-enhanced Raman spectroscopy. , 2016, Biosensors & bioelectronics.

[29]  Amany A. El-Hawwary,et al.  Hepatoprotective effects of cod liver oil against sodium nitrite toxicity in rats , 2013, Pharmaceutical biology.

[30]  P. Niedzielski,et al.  A new tool for inorganic nitrogen speciation study: simultaneous determination of ammonium ion, nitrite and nitrate by ion chromatography with post-column ammonium derivatization by Nessler reagent and diode-array detection in rain water samples. , 2006, Analytica chimica acta.

[31]  A. Mohamed,et al.  A novel kinetic determination of nitrite based on the perphenazine-bromate redox reaction , 2007 .

[32]  Hong-jun Han,et al.  Nitrite accumulation performance of aerobic MBBR treating Lurgi coal gasification waste water by adjusting pollutant load and DO concentration , 2015, Environmental technology.

[33]  T. Y. Chan.  Food-borne nitrates and nitrites as a cause of methemoglobinemia. , 1996, The Southeast Asian journal of tropical medicine and public health.

[34]  Amitava Das,et al.  Diamine derivative of a ruthenium(II)-polypyridyl complex for chemodosimetric detection of nitrite ion in aqueous solution , 2011 .

[35]  Jahan B. Ghasemi,et al.  Kinetic Spectrophotometric Determination of Nitrite Based on Its Catalytic Effect on the Oxidation of Methyl Red by Bromate , 2004 .

[36]  D. Froehlich,et al.  Effect of Nitrite and Salt on the Color, Flavor and Overall Acceptability of Ham , 1983 .

[37]  D. Tsikas.  Analysis of nitrite and nitrate in biological fluids by assays based on the Griess reaction: appraisal of the Griess reaction in the L-arginine/nitric oxide area of research. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[38]  L. Skibsted,et al.  Nitric oxide and myoglobins. , 2002, Chemical reviews.

[39]  Chenxu Yu,et al.  Rapid-response and highly sensitive noncross-linking colorimetric nitrite sensor using 4-aminothiophenol modified gold nanorods. , 2010, Analytical chemistry.

[40]  Y. Yue,et al.  Facile colorimetric detection of nitrite based on anti-aggregation of gold nanoparticles , 2015 .

[41]  Martha B. Shinn,et al.  Colorimetric Method for Determination of Nitrate , 1941 .

[42]  B. Narayana,et al.  A new system for the spectrophotometric determination of trace amounts of nitrite in environmental samples , 2006 .

[43]  Fang Fang,et al.  Sensitive paper-based analytical device for fast colorimetric detection of nitrite with smartphone , 2018, Analytical and Bioanalytical Chemistry.