TeraFly: real-time three-dimensional visualization and annotation of terabytes of multidimensional volumetric images

networks, coexpression to rescue RNA interference– or CRISPRCAS9–induced reduction of endogenous transcripts, and expression of ORFs carrying a mutation of interest to allow measurement of the mutation effect in the absence of the wild-type background. High-level gene coverage, combined with the versatility of Gateway cloning, and full access to OC clones make this collection a unique and valuable resource for the scientific community that should aid in the functional characterization of new protein targets and testing of disease-relevant mutations on a large scale. The OC resource will continue to be expanded in the future to increase human gene coverage, provide additional isoforms where available, provide clones with medically relevant mutations and add additional species, including ORFs from Xenopus and Drosophila.