Inhibition of phosphorylation of cellular dUTP nucleotidohydrolase as a consequence of herpes simplex virus infection

During an infection with herpes simplex virus, activity of cellular dUTPase decreases as a function of time, post‐infection, while virus‐encoded dUTPase activity increases. Prelabeling of cells with 35S‐methionine and immunoprecipitation analysis, using monoclonal antibodies, indicates that cellular dUTPase protein levels remain the same (with respect to levels in uninfected cells) throughout the infection period. New synthesis of cellular dUTPase does not occur in infected cells as determined by 35S‐methionine labeling during infection.

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