Integral proteins of the extracellular matrix fibrils of Myxococcus xanthus

The extracellular matrix fibrils of Myxococcus xanthus are mediators of cell-cell cohesion and as such are required for the maintenance of the social lifestyle characteristic of these prokaryotes. The fibrils have also been implicated as factors involved in contact-mediated cell interactions and in signal exchange. The fibrils are extracellular carbohydrate structures with associated proteins. All of the major proteins associated with the fibrils react with monoclonal antibody 2105 and can be removed from the fibrils only by boiling with sodium dodecyl sulfate (SDS) and beta-mercaptoethanol. For consistency with their integral association with the fibrils, we have designated this class of proteins as integral fibrillar proteins class 1 (IFP-1). IFP-1 comprises five major proteins whose molecular sizes range from 66 to 14 kDa. All of the proteins in IFP-1 have been purified from isolated fibrils by electroelution after size separation on SDS-PAGE gels. Analysis of the purified proteins suggested that the forms with different molecular sizes result from the aggregation of a single small-molecular-size subunit. Fingerprint analysis and amino acid composition profiles confirmed the identity among the different members of IFP-1. The sequence of the 31 amino-terminal amino acids of the 31-kDa form of IFP-1 (IFP-1:31) was determined. There was no significant homology to other known protein sequences. During development there is a dramatic shift in the banding pattern of IFP-1 proteins without any apparent overall loss of total protein.

[1]  D. Kaiser,et al.  Cell interactions in myxobacterial growth and development. , 1985, Science.

[2]  M. Dworkin,et al.  Synthesis of several membrane proteins during developmental aggregation in Myxococcus xanthus , 1982, Journal of bacteriology.

[3]  D. Kaiser,et al.  Fruiting body morphogenesis in submerged cultures of Myxococcus xanthus , 1982, Journal of bacteriology.

[4]  D. Kaiser,et al.  Social gliding is correlated with the presence of pili in Myxococcus xanthus. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[5]  K. Nikaido,et al.  Two-dimensional gel electrophoresis of membrane proteins. , 1976, Biochemistry.

[6]  L. Shimkets,et al.  Inhibition of cell-cell interactions in Myxococcus xanthus by congo red , 1988, Journal of bacteriology.

[7]  D. White,et al.  Cell surface modifications induced by calcium ion in the myxobacterium Stigmatella aurantiaca , 1992, Journal of bacteriology.

[8]  Dale Kaiser,et al.  Cell movement and its coordination in swarms of myxococcus xanthus , 1983 .

[9]  M. Kirschner,et al.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. , 1977, The Journal of biological chemistry.

[10]  D. Kaiser,et al.  Nutrition of Myxococcus xanthus, a fruiting myxobacterium , 1978, Journal of bacteriology.

[11]  W. Fluegel SIMPLE METHOD FOR DEMONSTRATING MYXOBACTERIAL SLIME , 1963, Journal of bacteriology.

[12]  P. Maeba Iodination of Myxococcus xanthus during development , 1983, Journal of Bacteriology.

[13]  M. Eisenbach,et al.  Effect of mechanical removal of pili on gliding motility of Myxococcus xanthus , 1992, Journal of bacteriology.

[14]  W. Vann,et al.  Translocation of capsular polysaccharides in pathogenic strains of Escherichia coli requires a 60-kilodalton periplasmic protein , 1987, Journal of bacteriology.

[15]  E. Stellwag,et al.  Monoclonal antibodies against cell-surface antigens of developing cells of Myxococcus xanthus. , 1985, Annales de l'Institut Pasteur. Microbiologie.

[16]  L. Shimkets,et al.  CsgA, an extracellular protein essential for Myxococcus xanthus development , 1990, Journal of bacteriology.

[17]  M. Dworkin,et al.  Biochemical and structural analyses of the extracellular matrix fibrils of Myxococcus xanthus , 1994, Journal of bacteriology.

[18]  M. Dunn Gel electrophoresis : proteins , 1993 .

[19]  J. Arnold,et al.  Mechanism of coaggregation between Actinomyces viscosus T14V and Streptococcus sanguis 34 , 1978, Infection and immunity.

[20]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[21]  M. Dworkin,et al.  A development-specific protein in Myxococcus xanthus is associated with the extracellular fibrils , 1991, Journal of bacteriology.

[22]  M. Dworkin,et al.  Cell density-dependent growth of Myxococcus xanthus on casein , 1977, Journal of bacteriology.

[23]  L. Shimkets,et al.  Regulation of cohesion-dependent cell interactions in Myxococcus xanthus , 1993, Journal of bacteriology.

[24]  E. Stackebrandt,et al.  Proteobacteria classis nov., a Name for the Phylogenetic Taxon That Includes the “Purple Bacteria and Their Relatives” , 1988 .

[25]  Robert P. Burchard,et al.  Gliding Motility and Taxes , 1984 .

[26]  T. MacRae,et al.  Evidence for motility-related fimbriae in the gliding microorganism Myxococcus xanthus. , 1976, Canadian journal of microbiology.

[27]  L. Shimkets,et al.  Effect of dsp mutations on the cell-to-cell transmission of CsgA in Myxococcus xanthus , 1993, Journal of bacteriology.

[28]  M. Dworkin,et al.  Extracellular fibrils and contact-mediated cell interactions in Myxococcus xanthus , 1991, Journal of bacteriology.

[29]  L. Shimkets,et al.  Cell surface properties correlated with cohesion in Myxococcus xanthus , 1988, Journal of bacteriology.

[30]  J. Holmgren,et al.  Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli , 1981, Journal of bacteriology.