Synthesis of [3α-3H]-dehydroepiandrosterone and [3α-3H]-pregnenolone

[3α-3H]-Dehydroepiandrosterone ([3α-3H]-3β-hydroxy-5-androsten-17-one) and [3α-3H]-pregnenolone ([3α-3H]-3β-hydroxy-5-pregnen-20-one) were prepared by selective reduction of 3-keto-5-ene intermediates with tritiated sodium borohydride. These were used as substrates to set up a tritium release assay for 3β-hydroxysteroid oxido-reductase and 54-ene isomerase (3β-HSD) which is a key enzyme in steroidogenesis. © 1998 John Wiley & Sons, Ltd.

[1]  C. Blais,et al.  Involvement of a 3β-hydroxysteroid dehydrogenase activity in ecdysteroid biosynthesis , 1997, Molecular and Cellular Endocrinology.

[2]  I. Bird,et al.  The regulation of 3β-hydroxysteroid dehydrogenase expression , 1997, Steroids.

[3]  E. Baulieu,et al.  A key enzyme in the biosynthesis of neurosteroids, 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase (3 beta-HSD), is expressed in rat brain. , 1995, Brain Research. Molecular Brain Research.

[4]  J. Simard,et al.  Identification and characterization of the G15D mutation found in a male patient with 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) deficiency: alteration of the putative NAD-binding domain of type II 3 beta-HSD. , 1995, Biochemistry.

[5]  M. Feuilloley,et al.  Immunocytochemical localization and biological activity of 3 beta- hydroxysteroid dehydrogenase in the central nervous system of the frog , 1994, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[6]  M. Skinner,et al.  Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes. , 1994, The Journal of general virology.

[7]  Minoru Tanaka,et al.  Ovarian 3,β‐hydroxysteroid dehydrogenase/Δ5‐4‐isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell , 1994 .

[8]  J. Simard,et al.  Detection and functional characterization of the novel missense mutation Y254D in type II 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) gene of a female patient with nonsalt-losing 3 beta HSD deficiency. , 1994, The Journal of clinical endocrinology and metabolism.

[9]  A. Payne,et al.  Enzyme characteristics of two distinct forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase complementary deoxyribonucleic acids expressed in COS-1 cells. , 1993, Endocrinology.

[10]  W. Rainey,et al.  3 beta-Hydroxysteroid dehydrogenase activity in glandular and extraglandular human fetal tissues. , 1991, The Journal of clinical endocrinology and metabolism.

[11]  C. Kotts,et al.  Activation of human placental 5-pregnene-3,20-dione isomerase activity by pyridine nucleotides. , 1988, Journal of steroid biochemistry.

[12]  Inano Hiroshi,et al.  Testicular and adrenal 3β-hydroxy-5-ene-steroid dehydrogenase and 5-ene-4-ene isomerase , 1987 .