论文信息 - Noncontact microsurgery of living cell membrane using femtosecond laser pulses

Noncontact microsurgery of living cell membrane using femtosecond laser pulses

Near-infrared femtosecond laser pulses were applied to initiate reversible permeabilization of cell membrane and inject extrinsic substances into the target cells. Successful laser-based injection of a membrane impermeable dye, as well as plasmid DNA was demonstrated.

[1] B Agate,et al. Femtosecond optical transfection of cells: viability and efficiency. , 2006, Optics express.

[2] A. Ovchinnikov,et al. Biomedical and biotechnology applications of noncontact femtosecond laser microsurgery of living cells , 2012 .

[3] Dmitry S. Sitnikov,et al. Noncontact microsurgery and micromanipulation of living cells with combined system femtosecond laser scalpel-optical tweezers , 2012, Photonics Europe.

[4] Karsten König,et al. Targeted transfection of stem cells with sub-20 femtosecond laser pulses. , 2008, Optics express.

[5] Kazuyoshi Itoh,et al. Femtosecond laser disruption of subcellular organelles in a living cell. , 2004, Optics express.

[6] Hajime Takano,et al. Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor , 2006, Nature Methods.

[7] Vikram Kohli,et al. Cell nanosurgery using ultrashort (femtosecond) laser pulses: Applications to membrane surgery and cell isolation , 2005, Lasers in surgery and medicine.

[8] Eric Mazur,et al. Ablation of cytoskeletal filaments and mitochondria in live cells using a femtosecond laser nanoscissor. , 2005, Mechanics & chemistry of biosystems : MCB.

[9] Holger Lubatschowski,et al. Quantified Femtosecond Laser Based Opto-perforation of Living Cells , 2009 .

[10] J. Eberwine,et al. Mammalian cell transfection: the present and the future , 2010, Analytical and bioanalytical chemistry.