Dual culture of Solanum tuberosum and Synchytrium endobioticum (pathotype 2)

Synchytrium endobioticum, the infective agent for wart disease of potato, is an obligate patho- gen establishing itself successfully on members of the Solanaceae. Its obligate nature and the absence of a monoxenic culture system have limited the level of biological knowledge attainable. We describe a mon- oxenic culture system using micropropagated potato plantlets. The pathogen was transferred successfully to a second set of plantlets in vitro which yielded resting spores, thus satisfying Koch's postulates for this disease.

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