Evidence for differential cellular adhesion as the mechanism of sorting-out of various cellular slime mold species.

Various authors have shown previously that if the amoebae of two species of slime molds are mixed they have the ability to sort themselves out. In the work reported here, the sorting-out of cells of several slime mold species was examined in clumps of cells in suspension. Cells of four species, Dictyostelium discoideum (Dd), D. mucoroides (Dm), D. purpureum (Dp), and Polysphondylium violaceum (Pv), were mixed in pairs in suspension and clumps of cells formed. Dd and Pv cells sorted out completely and formed separate clumps, each of single species. Both Dd and Dm, when mixed separately with Dp, formed clumps containing both species. Sorting-out took place in these clumps such that the cells of Dd and Dm partially enveloped the Dp cells. Finally, in the Dd-Dm mixtures, the Dm cells always sorted out such that they surrounded the Dd cells. When mixed in a 1:2 ratio (Dd:Dm) the Dm cells formed a complete shell around a sphere of Dd cells. Sorting-out of cells in clumps in suspension can occur by either of two possible mechanisms: response of cells to a chemotactic gradient or differences in cell surface strengths of adhesion (Steinberg, 1964). Mixing of two species in a clump of cells and observing the process of sorting-out permits one to distinguish between these two mechanisms (Steinberg, 1964). By such an analysis it was found that the sorting-out observed in mixtures of Dd and Dm is consistent with the mechanism of differential cellular adhesion. The major reasons for this are (1) when the adhesive properties of the cells are known to change the Dd cells began to move inside the clumps, (2) the Dd cells coalesced into islands rather than streaming inward independently, and (3) the Dd cells and cell masses did not lie at the center of the clumps but rather lay randomly within the clumps. The partial envelopment observed in the Dd-Dp and Dm-Dp mixtures and the separate clumps formed by the Dd-Pv mixtures are also consistent with differential cellular adhesion. They represent cases in which the interspecific strengths of adhesion are low (Dd-Dp and Dm-Dp) and near zero (Dv-Pv).

[1]  S. Barondes,et al.  Direct measurement of species-specific cohesion in cellular slime molds , 1978, The Journal of cell biology.

[2]  G. Gerisch,et al.  A specific glycoprotein as the target site of adhesion blocking Fab in aggregating Dictyostelium cells , 1978, Nature.

[3]  D. Garrod,et al.  Mutual cohesion and cell sorting-out among four species of cellular slime moulds. , 1978, Journal of cell science.

[4]  M. Brenner,et al.  Cyclic AMP levels and turnover during development of the cellular slime mold Dictyostelium discoideum. , 1978, Developmental biology.

[5]  J. Bonner,et al.  Preliminary characterization of the acrasin of the cellular slime mold Polysphondylium violaceum. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[6]  S. Barondes,et al.  Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[7]  J. Ashworth,et al.  Cell sorting out during the differentiation of mixtures of metabolically distinct populations of Dictyostelium discoideum. , 1973, Journal of embryology and experimental morphology.

[8]  J. Bonner,et al.  Synthesis of cyclic AMP and phosphodiesterase in various species of cellular slime molds and its bearing on chemotaxis and differentiation. , 1972, Developmental biology.

[9]  M. S. Steinberg,et al.  Experimental modulation of intercellular cohesiveness: reversal of tissue assembly patterns. , 1972, Developmental biology.

[10]  J. Bonner,et al.  Further evidence for the sorting out of cells in the differentiation of the cellular slime mold Dictyostelium discoideum. , 1971, Journal of embryology and experimental morphology.

[11]  M. S. Steinberg,et al.  Does differential adhesion govern self-assembly processes in histogenesis? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells. , 1970, The Journal of experimental zoology.

[12]  J. Bonner,et al.  The acrasin activity of adenosine-3',5'-cyclic phosphate. , 1967, Proceedings of the National Academy of Sciences of the United States of America.

[13]  J. Bonner.  EVIDENCE FOR THE SORTING OUT OF CELLS IN THE DEVELOPMENT OF THE CELLULAR SLIME MOLDS. , 1959, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Bonner,et al.  Cell mixtures of different species and strains of cellular slime moulds. , 1958, Journal of embryology and experimental morphology.

[15]  Philip L. Townes,et al.  Directed movements and selective adhesion of embryonic amphibian cells , 1955 .

[16]  J. Bonner.  The demonstration of acrasin in the later stages of the development of the slime mold Dictyostelium discoideum. , 1949, The Journal of experimental zoology.

[17]  J. Bonner,et al.  Evidence for the formation of cell aggregates by chemotaxis in the development of the slime mold Dictyostelium discoideum. , 1947, The Journal of experimental zoology.

[18]  M. Sussman,et al.  Cellular Slime Molds , 1974 .

[19]  J. Bonner.  Aggregation and differentiation in the cellular slime molds. , 1971, Annual review of microbiology.

[20]  G Gerisch,et al.  Cell aggregation and differentiation in Dictyostelium. , 1968, Current topics in developmental biology.

[21]  Malcolm S. Steinberg,et al.  The Problem of Adhesive Selectivity in Cellular Interactions , 1964 .

[22]  K. Raper,et al.  INTERSPECIFIC MIXTURES IN THE DICTYOSTELIACEAE , 1941 .