The distribution and orientation of noradrenergic fibers in neocortex of the rat: An immunofluorescence study

A homologous antiserum directed against rat dopamine‐β‐hydroxylase (DBH) was employed in the indirect immunofluorescence technique of Coons ('58) to describe the distribution and orientation of noradrenergic (NA) axons in the cerebral neocortex of the adult albino rat. DBH is the terminal enzyme in the biosynthesis of noradrenaline and is a sensitive anatomic marker for NA neurons and their processes. Cryostat sections in three standard planes (coronal, sagittal and tangential) taken from four cortical fields (somatosensory, motor, visual and anterior cingulate) were processed for immunofluorescence, and adjacent sections were stained with a Nissl method to relate the pattern of NA axons to specific cortical laminae. Noradrenaline levels and DBH activity were measured in the same cortical fields by radioenzymatic assays.

[1]  T. Powell,et al.  The intrinsic, association and commissural connections of area 17 on the visual cortex. , 1975, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[2]  U. Ungerstedt.  Stereotaxic mapping of the monoamine pathways in the rat brain. , 1971, Acta physiologica Scandinavica. Supplementum.

[3]  D. Jacobowitz,et al.  Topographic atlas of catecholamine and acetylcholinesterase‐containing neurons in the rat brain. I. Forebrain (telencephalon, diencephalon) , 1974, The Journal of comparative neurology.

[4]  O. Johansson,et al.  Dopamine Nerve Terminals in the Rat Limbic Cortex: Aspects of the Dopamine Hypothesis of Schizophrenia , 1974, Science.

[5]  T. Powell,et al.  On the thalamo-cortical connexions of the general sensory cortex of Macaca , 1953, Proceedings of the Royal Society of London. Series B - Biological Sciences.

[6]  T. Powell,et al.  An electron microscopic study of the laminar pattern and mode of termination of afferent fibre pathways in the somatic sensory cortex of the cat. , 1970, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[7]  J. Morrison,et al.  The immunohistochemical demonstration of noradrenergic neurons in the rat brain: The use of homologous antiserum to dopamine-β-hydroxylase , 1977, Neuroscience Letters.

[8]  B. K. Hartman,et al.  The use of dopamine -hydroxylase as a marker for the central noradrenergic nervous system in rat brain. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Coyle,et al.  RAT ADRENAL DOPAMINE‐β‐HYDROXYLASE PURIFICATION AND IMMUNOLOGIC CHARACTERISTICS , 1976 .

[10]  D. Reis,et al.  Monoamine-synthesizing enzymes in central dopaminergic, noradrenergic and serotonergic neurons. Immunocytochemical localization by light and electron microscopy. , 1976, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[11]  Laurent Descarries,et al.  Noradrenergic axon terminals in the cerebral cortex of rat. III. Topometric ultrastructural analysis , 1977, Brain Research.

[12]  F E Bloom,et al.  Histochemical characterization of a neocortical projection of the nucleus locus coeruleus in the squirrel monkey , 1975, The Journal of comparative neurology.

[13]  F. Bloom,et al.  Studies on norepinephrine-containing afferents to Purkinje cells of rat cerebellum. II. Sensitivity of Purkinje cells to norepinephrine and related substances administered by microiontophoresis. , 1971, Brain research.

[14]  D. Jacobowitz,et al.  Quantitative relationship of catecholamine content and histofluorescence in brain of rats. , 1974, Brain research.

[15]  F. Bloom,et al.  A radioautographic study of the efferent pathways of the nucleus locus coeruleus , 1974, The Journal of comparative neurology.

[16]  F. Bloom,et al.  Studies on norepinephrine-containing afferents to Purkinje cells of art cerebellum. I. Localization of the fibers and their synapses. , 1971, Brain research.

[17]  Barbara E. Jones,et al.  Ascending projections of the locus coeruleus in the rat. II. Autoradiographic study , 1977, Brain Research.

[18]  M. Molliver,et al.  The fine structural demonstration of monoaminergic synapses in immature rat neocortex , 1975, Neuroscience Letters.

[19]  B. K. Hartman.  THE JOURNAL OF HiSTOCHEMiSTRY AND CYTOCHEMiSTRY , 2005 .

[20]  D H Hubel,et al.  Autoradiographic demonstration of ocular-dominance columns in the monkey striate cortex by means of transneuronal transport. , 1974, Brain research.

[21]  E. G. Jones,et al.  Midbrain, diencephalic and cortical relationships of the basal nucleus of meynert and associated structures in primates , 1976, The Journal of comparative neurology.

[22]  Lars Olson,et al.  Ascending Monoamine Neurons to the Telencephalon and Diencephalon , 1966 .

[23]  L. Sternberger,et al.  THE UNLABELED ANTIBODY ENZYME METHOD OF IMMUNOHISTOCHEMISTRY PREPARATION AND PROPERTIES OF SOLUBLE ANTIGEN-ANTIBODY COMPLEX (HORSERADISH PEROXIDASE-ANTIHORSERADISH PEROXIDASE) AND ITS USE IN IDENTIFICATION OF SPIROCHETES , 1970, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[24]  E. G. Jones,et al.  The organization and postnatal development of the commissural projection of the rat somatic sensory cortex , 1976, The Journal of comparative neurology.

[25]  T. Maeda,et al.  Detailed noradrenaline pathways of locus coeruleus neuron to the cerebral cortex with use of 6-hydroxydopa. , 1974, Brain research.

[26]  H. Burton,et al.  Areal differences in the laminar distribution of thalamic afferents in cortical fields of the insular, parietal and temporal regions of primates , 1976, The Journal of comparative neurology.

[27]  V. Mountcastle.  Modality and topographic properties of single neurons of cat's somatic sensory cortex. , 1957, Journal of neurophysiology.

[28]  S. Nakamura.  Some electrophysiological properties of neurones of rat locus coeruleus. , 1977, The Journal of physiology.

[29]  J. Coyle,et al.  CATECHOLAMINES IN FETAL AND NEWBORN RAT BRAIN , 1973, Journal of neurochemistry.

[30]  W. Krieg.  Connections of the cerebral cortex. I. The albino rat. A. Topography of the cortical areas , 1946 .

[31]  H. Killackey.  Anatomical evidence for cortical subdivisions based on vertically discrete thalamic projections from the ventral posterior nucleus to cortical barrels in the rat. , 1973, Brain research.

[32]  J. Price,et al.  The cortical projections of the mediodorsal nucleus and adjacent thalamic nuclei in the rat , 1977, The Journal of comparative neurology.

[33]  O. Lindvall,et al.  The catecholamine systems in the developing rat brain: improved visualization by a modified glyoxylic acid-formaldehyde method , 1976, Brain Research.

[34]  B. K. Hartman,et al.  The central adrenergic system. An immunofluorescence study of the location of cell bodies and their efferent connections in the rat utilizing dopamine‐B‐hydroxylase as a marker , 1975, The Journal of comparative neurology.

[35]  P. Molinoff,et al.  A sensitive enzymatic assay for dopamine- -hydroxylase. , 1971, The Journal of pharmacology and experimental therapeutics.

[36]  M. Palkovits,et al.  Regional concentrations of noradrenaline and dopamine in rat brain , 1976, Brain Research.

[37]  J. Price,et al.  A direct input from the amygdala to the thalamus and the cerebral cortex. , 1974, Brain research.

[38]  P. Molinoff,et al.  The normal occurrence of octopamine in the central nervous system of the rat , 1977, Brain Research.

[39]  J. Szentágothai.  Synaptology of the Visual Cortex , 1973 .

[40]  M. Palkovits,et al.  Regional distribution of adrenaline in rat brain , 1976, Brain Research.

[41]  J. Coyle,et al.  Major innervation of newborn rat cortex by monoaminergic neurons. , 1977, Science.

[42]  L. Loizou,et al.  Projections of the nucleus locus coeruleus in the albino rat. , 1969, Brain research.

[43]  T. Powell,et al.  The projection of the locus coeruleus upon the neocortex in the macaque monkey , 1977, Neuroscience.

[44]  W. Nauta,et al.  Columnar distribution of cortico-cortical fibers in the frontal association, limbic, and motor cortex of the developing rhesus monkey , 1977, Brain Research.

[45]  T. Hökfelt,et al.  Immunohistochemical studies on monoamine-containing cell systems. , 1973, Brain research.

[46]  G. Werner,et al.  Topology of the body representation in somatosensory area I of primates. , 1968, Journal of neurophysiology.

[47]  T. Powell,et al.  The cortical projection of the ventroposterior nucleus of the thalamus in the cat. , 1969, Brain research.

[48]  T. Maeda,et al.  Projections ascendantes du locus coeruleus et d'autres neurones aminergiques pontiques au niveau du prosencéphale du rat , 1972 .

[49]  K Fuxe,et al.  Distribution of noradrenaline nerve terminals in cortical areas of the rat. , 1968, Brain research.

[50]  G. Thieme,et al.  FLUORESCENCE OF CATECHOL AMINES AND RELATED COMPOUNDS CONDENSED WITH FORMALDEHYDE , 1962 .

[51]  T. Hökfelt,et al.  Cellular Localization of Dopamine-β-hydroxylase and Phenylethanolamine-N-methyl Transferase as Revealed by Immunohistochemistry , 1971 .

[52]  D. Hubel,et al.  Laminar and columnar distribution of geniculo‐cortical fibers in the macaque monkey , 1972, The Journal of comparative neurology.

[53]  O. Lindvall,et al.  The adrenergic innervation of the rat thalamus as revealed by the glyoxylic acid fluorescence method , 1974, The Journal of comparative neurology.

[54]  J. Coyle,et al.  Visualization of central noradrenergic neurons in thick sections by the unlabeled antibody method: a transmitter-specific Golgi image. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[55]  D. Jacobowitz,et al.  Biochemical mapping of noradrenergic nerves arising from the rat locus coeruleus. , 1974, Brain research.

[56]  E. G. Jones,et al.  Lamination and differential distribution of thalamic afferents within the sensory‐motor cortex of the squirrel monkey , 1975, The Journal of comparative neurology.

[57]  J. Glowinski,et al.  Dopaminergic Terminals in the Rat Cortex , 1973, Science.

[58]  R. Rush,et al.  Immunohistochemical localization of protein components of catecholamine storage vesicles , 1969 .

[59]  N. Zečević,et al.  Characterization of the monoaminergic innervation of immature rat neocortex: A histofluorescence analysis , 1978, The Journal of comparative neurology.

[60]  H. Kuypers,et al.  Basal forebrain and hypothalamic connection to frontal and parietal cortex in the Rhesus monkey. , 1975, Science.