Computerized Analysis Of Two-Dimensional Electrophoretic Images

Computerized analysis of 2-D gel electrophoretic images is the basic approach to solve various problems involved in the interpretation of the results of this technique. In this paper many aspects are investigated: smoothing procedures, relation between protein quantities and integrated optical densities, segmentation techniques, and wide feature extraction, pointing out also the importance of spatial resolution. In particular, a newly developed segmentation technique is applied to the smoothed images and the promising results are discussed.