Neutrophil polarity and locomotion are associated with surface redistribution of leukosialin (CD43), an antiadhesive membrane molecule.

This study analyzed the behavior of an antiadhesive membrane molecule, CD43, in neutrophil polarization and locomotion. CD43 cross-linking by antibodies induced neutrophil locomotion, with CD43 molecules clustered at the uropod of polarized neutrophils. In contrast, CD11b/CD18 cross-linking by antibodies did not affect either cell polarization or locomotion. Stimulation of suspended or adherent neutrophils with chemotactic peptide results in cell polarization and locomotion and a concomitant redistribution of CD43 to the uropod. This process is entirely reversible. The study also investigated which actin-binding protein could be involved in CD43 lateral redistribution. alpha-Actinin and moesin are preferentially adsorbed on Sepharose beads bearing a recombinant CD43 intracellular domain. Analysis by immunofluorescence confocal microscopy shows a codistribution of moesin during CD43 lateral redistribution. By contrast, alpha-actinin is located at the leading edge, an area devoid of CD43. These results shed new light on the role of CD43 membrane redistribution, which appears to be directly related to neutrophil polarity and locomotion. (Blood. 2000;95:2462-2470)

[1]  P. Kubes,et al.  The Functional Paradox of CD43 in Leukocyte Recruitment: A Study Using CD43-deficient Mice , 1998, The Journal of experimental medicine.

[2]  R. Barth,et al.  The Roman god Janus: a paradigm for the function of CD43. , 1998, Immunology today.

[3]  Friedl,et al.  Direct and rapid induction of migration in human CD4+ T lymphocytes within three‐dimensional collagen matrices mediated by signalling via CD3 and/or CD2 , 1998, Immunology.

[4]  K. Zänker,et al.  CD4+ T lymphocytes migrating in three‐dimensional collagen lattices lack focal adhesions and utilize β1 integrin‐independent strategies for polarization, interaction with collagen fibers and locomotion , 1998, European journal of immunology.

[5]  F. Sánchez‐Madrid,et al.  CD43 interacts with moesin and ezrin and regulates its redistribution to the uropods of T lymphocytes at the cell-cell contacts. , 1998, Blood.

[6]  P. Bongrand,et al.  Adhesion‐related glycocalyx study: quantitative approach with imaging‐spectrum in the energy filtering transmission electron microscope (EFTEM) , 1998, FEBS letters.

[7]  Sean P. Palecek,et al.  Physical and biochemical regulation of integrin release during rear detachment of migrating cells. , 1998, Journal of cell science.

[8]  N. Takahashi,et al.  Ezrin/Radixin/Moesin (ERM) Proteins Bind to a Positively Charged Amino Acid Cluster in the Juxta-Membrane Cytoplasmic Domain of CD44, CD43, and ICAM-2 , 1998, The Journal of cell biology.

[9]  Z. Werb.  ECM and Cell Surface Proteolysis: Regulating Cellular Ecology , 1997, Cell.

[10]  L. Halbwachs‐Mecarelli,et al.  Leukosialin (CD43, sialophorin) redistribution in uropods of polarized neutrophils is induced by CD43 cross-linking by antibodies, by colchicine or by chemotactic peptides. , 1997, Journal of cell science.

[11]  P. Bongrand,et al.  Leukosialin (CD43) behavior during adhesion of human monocytic THP‐1 cells to red blood cells , 1997, Journal of leukocyte biology.

[12]  G. Zimmerman,et al.  Leukocyte activation induces surface redistribution of P‐selectin glycoprotein ligand‐1 , 1997, Journal of leukocyte biology.

[13]  Sean P. Palecek,et al.  Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesiveness , 1997, Nature.

[14]  J. Holgersson,et al.  Triggering of motile behavior in T lymphocytes via cross-linking of alpha 4 beta 1 and alpha L beta 2. , 1997, Journal of immunology.

[15]  M. Bretscher.  Getting Membrane Flow and the Cytoskeleton to Cooperate in Moving Cells , 1996, Cell.

[16]  A. Huttenlocher,et al.  Modulation of cell migration by integrin-mediated cytoskeletal linkages and ligand-binding affinity , 1996, Journal of Cell Biology.

[17]  T. Mitchison,et al.  Actin-Based Cell Motility and Cell Locomotion , 1996, Cell.

[18]  D. Lauffenburger,et al.  Cell Migration: A Physically Integrated Molecular Process , 1996, Cell.

[19]  B. Gumbiner,et al.  Cell Adhesion: The Molecular Basis of Tissue Architecture and Morphogenesis , 1996, Cell.

[20]  N. Manjunath,et al.  Negative regulation of T-cell adhesion and activation by CD43 , 1995, Nature.

[21]  F. Sánchez‐Madrid,et al.  Regulatory role of CD43 leukosialin on integrin-mediated T-cell adhesion to endothelial and extracellular matrix ligands and its polar redistribution to a cellular uropod. , 1995, Blood.

[22]  F. Maxfield,et al.  Ca2+- and calcineurin-dependent recycling of an integrin to the front of migrating neutrophils , 1995, Nature.

[23]  K. Pestonjamasp,et al.  Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes. , 1995, Molecular biology of the cell.

[24]  E. Elson,et al.  A mechanical function of myosin II in cell motility. , 1995, Journal of cell science.

[25]  M. Sheetz,et al.  Cell migration by graded attachment to substrates and contraction. , 1994, Seminars in cell biology.

[26]  Paul Kubes,et al.  The microcirculation and inflammation: modulation of leukocyte‐endothelial cell adhesion , 1994, Journal of leukocyte biology.

[27]  N. Sato,et al.  Concentration of an integral membrane protein, CD43 (leukosialin, sialophorin), in the cleavage furrow through the interaction of its cytoplasmic domain with actin-based cytoskeletons , 1993, The Journal of cell biology.

[28]  J. Condeelis,et al.  Life at the leading edge: the formation of cell protrusions. , 1993, Annual review of cell biology.

[29]  A. Horwitz,et al.  Dynamics of beta 1 integrin-mediated adhesive contacts in motile fibroblasts , 1992, The Journal of cell biology.

[30]  P. Rieu,et al.  Human neutrophils release their major membrane sialoprotein, leukosialin (CD43), during cell activation , 1992, European journal of immunology.

[31]  John I. Gallin,et al.  Inflammation: Basic Principles and Clinical Correlates , 1992 .

[32]  D. Staunton,et al.  CD43 interferes with T-lymphocyte adhesion. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[33]  M. Bretscher,et al.  Circulating integrins: alpha 5 beta 1, alpha 6 beta 4 and Mac‐1, but not alpha 3 beta 1, alpha 4 beta 1 or LFA‐1. , 1992, The EMBO journal.

[34]  J. Condeelis.  Are all pseudopods created equal? , 1992, Cell motility and the cytoskeleton.

[35]  C. Turner,et al.  Transmembrane molecular assemblies in cell-extracellular matrix interactions. , 1991, Current opinion in cell biology.

[36]  F. Maxfield,et al.  Attachment to fibronectin or vitronectin makes human neutrophil migration sensitive to alterations in cytosolic free calcium concentration , 1991, The Journal of cell biology.

[37]  L. Osborn,et al.  Leukocyte adhesion to endothelium in inflammation , 1990, Cell.

[38]  D. Stüber,et al.  System for High-Level Production in Escherichia coli and Rapid Purification of Recombinant Proteins: Application to Epitope Mapping, Preparation of Antibodies, and Structure—Function Analysis , 1990 .

[39]  E. Remold-O’Donnell,et al.  Sialophorin (CD43) and the Wiskott-Aldrich syndrome. , 1990, Immunodeficiency reviews.

[40]  T. Stossel,et al.  From signal to pseudopod. How cells control cytoplasmic actin assembly. , 1989, The Journal of biological chemistry.

[41]  James A. Spudich,et al.  Capping of surface receptors and concomitant cortical tension are generated by conventional myosin , 1989, Nature.

[42]  M. Mattei,et al.  Characterization of cDNAs encoding human leukosialin and localization of the leukosialin gene to chromosome 16. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[43]  E. Remold-O’Donnell,et al.  Expression on blood cells of sialophorin, the surface glycoprotein that is defective in Wiskott-Aldrich syndrome. , 1987, Blood.

[44]  R. Clark,et al.  Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation , 1983, The Journal of cell biology.

[45]  E. Unanue,et al.  Anti-Ig-triggered movements of lymphocytes-specificity and lack of evidence for directional migration. , 1975, Journal of immunology.

[46]  E. Unanue,et al.  LIGAND-INDUCED MOVEMENT OF LYMPHOCYTE SURFACE MACROMOLECULES , 1974, The Journal of experimental medicine.