Acid helix‐turn activator motif

A common sequence/structural motif pattern has been identified within the steroid/thyroid hormone receptors and other transcriptional activators using a new massively parallel symbolic learning assistant computer system. The pattern appears nearly diagnostic of transcription activation, including relative activation strength, among nuclear and DNA‐binding prokaryotic proteins. In cases where mutation/deletion/chimeric studies have identified the activation domain, the pattern matches within that domain. These facts and the nature of the pattern itself strongly support the idea that the patterned domain is directly involved in a protein‐protein transcription activation interaction.

[1]  Mark Ptashne,et al.  Mutants of GAL4 protein altered in an activation function , 1987, Cell.

[2]  R. Evans,et al.  The steroid and thyroid hormone receptor superfamily. , 1988, Science.

[3]  M. Ptashne,et al.  Transcription in yeast activated by a putative amphipathic α helix linked to a DNA binding unit , 1987, Nature.

[4]  Jun Ma,et al.  GAL4-VP16 is an unusually potent transcriptional activator , 1988, Nature.

[5]  Michael R. Green,et al.  Transcription activation by the adenovirus E1a protein , 1989, Nature.

[6]  P. Chambon,et al.  The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activator , 1988, Cell.

[7]  Jun Ma,et al.  A new class of yeast transcriptional activators , 1987, Cell.

[8]  R. Tjian,et al.  Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. , 1989, Science.

[9]  J. Bishop,et al.  Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-myb , 1989, Cell.

[10]  D. Chasman,et al.  Activation of yeast polymerase II transcription by herpesvirus VP16 and GAL4 derivatives in vitro , 1989, Molecular and cellular biology.

[11]  I. Pastan,et al.  Molecular cloning and characterization of a human DNA binding factor that represses transcription , 1989, Cell.

[12]  R H Lathrop,et al.  Pattern descriptors and the unidentified reading frame 6 human mtDNA dinucleotide‐binding site , 1988, Proteins.

[13]  Jun Ma,et al.  Deletion analysis of GAL4 defines two transcriptional activating segments , 1987, Cell.

[14]  Kevin Struhl,et al.  Structural and functional characterization of the short acidic transcriptional activation region of yeast GCN4 protein , 1988, Nature.

[15]  R H Lathrop,et al.  Consensus topography in the ATP binding site of the simian virus 40 and polyomavirus large tumor antigens. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[16]  Richard H. Lathrop,et al.  ARIADNE: pattern-directed inference and hierarchical abstraction in protein structure recognition , 1987, CACM.

[17]  K. Struhl,et al.  Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast , 1986, Cell.

[18]  M. Ptashne.  How eukaryotic transcriptional activators work , 1988, Nature.

[19]  R. F. Smith,et al.  Automatic generation of primary sequence patterns from sets of related protein sequences. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[20]  W. Taylor,et al.  Identification of protein sequence homology by consensus template alignment. , 1986, Journal of molecular biology.

[21]  D Eisenberg,et al.  Hydrophobic organization of membrane proteins. , 1989, Science.

[22]  P. Chambon,et al.  The hormone-binding domains of the estrogen and glucocorticoid receptors contain an inducible transcription activation function , 1988, Cell.

[23]  R. Evans,et al.  Multiple and cooperative trans-activation domains of the human glucocorticoid receptor , 1988, Cell.