Influence of Oxygen Concentration and Mechanical Factors on Differentiation of Connective Tissues in vitro
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MARCHAND1 postulated that osteoblasts may differentiate from mesenchymal cells. Recently, it was observed that a strain of cells, derived from adult skeletal muscle, elaborated in tissue culture a sub-stance that appeared to be chondro-osteoid2. Since the factors responsible for this cell behaviour were unknown, investigation of the effects of varying mechanical and nutritional factors on a reproducible in vitro system of osteogenesis was made. Hanging-drop, Maximov double cover-slip cultures were established from 20-day-old chick embryo tibial cortex and were maintained as described by Fell3.
[1] W. Möllendorff,et al. Handbuch der Mikroskopischen Anatomie des Menschen , 1958 .
[2] R. Robinson,et al. CRYSTAL‐COLLAGEN RELATIONSHIPS IN BONE AS OBSERVED IN THE ELECTRON MICROSCOPE. III. CRYSTAL AND COLLAGEN MORPHOLOGY AS A FUNCTION OF AGE , 1955, Annals of the New York Academy of Sciences.
[3] H. B. Fell. The Osteogenic Capacity in vitro of Periosteum and Endosteum Isolated from the Limb Skeleton of Fowl Embryos and Young Chicks. , 1932, Journal of anatomy.