Hydrolytic enzyme production potential of bacterial population from Sahastradhara cold sulfur spring, Uttarakhand.

Hydrolytic potential of bacterial isolates from Sahastradhara sulfur spring was explored using qualitative approach. A total of 14 isolates, obtained by serial dilution and spread plate method, were purified by quadrate streaking. The isolates were characterized morphologically and biochemically. Bacterial population was comprised of rod shaped members. All the recovered isolates were Gram positive in nature and were screened for production of amylase, cellulase, lipase and protease. All the isolates exhibited amylolytic activity. 71.4%, 85.7% and 64.2% were positive for cellulase, lipase and protease respectively. Majority of isolates were positive for more than one hydrolytic activity. The percentage of isolates exhibiting single, two, three and four hydrolytic activities was 7.1%, 14.2%, 14.2% and 64.2% respectively. Larger proportion of population had good amylolytic potential. Enzyme index greater than one was shown by 42.8%, 28.5%, 14.2% and 21.4% isolates for amylase, cellulase, lipase and protease respectively.

[1]  N. Masngut,et al.  Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation , 2017 .

[2]  A. Marsaioli,et al.  Enzymatic potential of heterotrophic bacteria from a neutral copper mine drainage , 2016, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[3]  Sonika Gupta,et al.  Halophilic Bacteria of Lunsu Produce an Array of Industrially Important Enzymes with Salt Tolerant Activity , 2016, Biochemistry research international.

[4]  Padma Singh,et al.  Isolation and characterization of amylase producing Bacillus spp. from selected soil sample , 2016 .

[5]  M. Javed,et al.  Characterization Of A Novel Hydrolytic Enzyme Producing Thermophilic Bacterium Isolated From The Hot Spring Of Azad Kashmir-Pakistan , 2016 .

[6]  B. Khatri,et al.  ENZYMATIC SCREENING AND MOLECULAR CHARACTERIZATION OF THERMOPHILIC BACTERIAL STRAINS ISOLATED FROM HOTSPRING OF TATOPANI, BHURUNG, NEPAL , 2015 .

[7]  W. Rivera,et al.  Isolation and characterization of culturable thermophilic bacteria from hot springs in Benguet, Philippines , 2015 .

[8]  V. Nigam,et al.  PRODUCTION OF AMYLASE ENZYME BY ISOLATED MICROORGANISMS AND IT ’ S APPLICATION , 2014 .

[9]  C. Suganthi,et al.  Screening and optimization of protease production from a halotolerant Bacillus licheniformis isolated from saltern sediments , 2013 .

[10]  B. Purushotham,et al.  ISOLATION, SCREENING AND CHARACTERIZATION OF PROMISING A-AMYLASE PRODUCING BACTERIA FROM SEWAGE ENRICHED SOIL , 2013 .

[11]  C. Farinas,et al.  Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains , 2012, Enzyme research.

[12]  Saima,et al.  Cold-active extracellular α-amylase production from novel bacteria Microbacterium foliorum GA2 and Bacillus cereus GA6 isolated from Gangotri glacier, Western Himalaya , 2012 .

[13]  Sumit Kumar,et al.  Brazilian Journal of Microbiology (2012): , 2013 .

[14]  Rishi Gupta,et al.  Microbial Cellulases and Their Industrial Applications , 2011, Enzyme research.

[15]  M. A. Amoozegar,et al.  Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake , 2011 .

[16]  M. Amoozegar,et al.  Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran , 2009, Journal of Industrial Microbiology & Biotechnology.

[17]  Tin-Chun Chu,et al.  Microbiology Laboratory Manual , 2008 .

[18]  Abdul Hameed,et al.  Industrial applications of microbial lipases , 2006 .

[19]  S. Arts.  Amylases and their applications , 2004 .

[20]  A. Ventosa,et al.  Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. , 2003, Journal of applied microbiology.

[21]  M. Rao,et al.  Molecular and Biotechnological Aspects of Microbial Proteases , 1998, Microbiology and Molecular Biology Reviews.

[22]  B. S. Chadha,et al.  Shake culture studies for the production of amylases by Thermomyces lanuginosus. , 1997, Acta microbiologica et immunologica Hungarica.

[23]  P. Wood,et al.  Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen , 1982, Applied and environmental microbiology.