Fluid dynamics within a rotating bioreactor in space and earth environments

A mathematical model was recently developed to characterize cell-medium interactions within a Couette-flow bioreactor. To test the efficiency of the model, numerical simulations and space-flight experiments have been conducted. In this study, the momentum equations for the steady-state fluid flow is solved first followed by the equations for the motion of a particle. Results showed that in unit gravity, the bioreactor would simulate primary microgravity trajectories of particles and migration time. However, by rotating the bioreactor under the influence of gravity produces a significant component of particle motion and associated shear stress not found in microgravity environment. In addition, the total force per unit of cross-sectional area on a particle in microgravity is significantly smaller than the calculated value in unit gravity. 17 refs.

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