Kinetics of chondrocyte growth in cell‐polymer implants

In vitro cultivation of cartilage cells (chondrocytes) on biodegradable polyglycolic acid (PGA) scaffolds resulted in implants which could potentially be used to repair damaged joint cartilage or for reconstructive surgery. Cell growth kinetics were studied to define conditions under which the cellularity of implants made from isolated calf chondrocytes reached that of the parent calf cartilage. In static cultures, condrocyte growth rates decreased as either implant thickness or implant cell density increased. Over 4 weeks of cultivation, implant permeability to glucose decreased to 3% that of the plain polymer scaffold; this effect was attributed to the decrease in effective implant porosity associated with cartilage tissue regeneration.

[1]  G. Vunjak‐Novakovic,et al.  Composition of cell‐polymer cartilage implants , 1994, Biotechnology and bioengineering.

[2]  D. Buttle,et al.  Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. , 1986, Biochimica et biophysica acta.

[3]  V. Pellegrini,et al.  Osteoarthritis of the trapeziometacarpal joint: the pathophysiology of articular cartilage degeneration. I. Anatomy and pathology of the aging joint. , 1991, The Journal of hand surgery.

[4]  A Ratcliffe,et al.  Cartilage and diarthrodial joints as paradigms for hierarchical materials and structures. , 1992, Biomaterials.

[5]  L. Peterson,et al.  The repair of experimentally produced defects in rabbit articular cartilage by autologous chondrocyte transplantation , 1989, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[6]  G. Vunjak‐Novakovic,et al.  Cultivation of cell‐polymer cartilage implants in bioreactors , 1993, Journal of cellular biochemistry.

[7]  A. Grodzinsky,et al.  Fluorometric assay of DNA in cartilage explants using Hoechst 33258. , 1988, Analytical biochemistry.

[8]  M. Spycher,et al.  Induction and prevention of chondrocyte hypertrophy in culture , 1989, The Journal of cell biology.

[9]  C. Ohlsson,et al.  Cellular aspects on treatment of cartilage injuries. , 1993, Agents and actions. Supplements.

[10]  A. Grodzinsky,et al.  Biosynthetic response of cartilage explants to dynamic compression , 1989, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[11]  R. Cancedda,et al.  Cell condensation in chondrogenic differentiation. , 1992, Experimental cell research.

[12]  R Langer,et al.  Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds. , 1994, Journal of biomedical materials research.

[13]  C. A. Poole,et al.  Chondrons in cartilage: Ultrastructural analysis of the pericellular microenvironment in adult human articular cartilages , 1987, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[14]  R Langer,et al.  Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. , 1993, Journal of biomedical materials research.

[15]  M. Solursh.  Formation of cartilage tissue in vitro , 1991, Journal of cellular biochemistry.

[16]  W. Thilly,et al.  MICROCARRIERS AND THE PROBLEM OF HIGH DENSITY CELL CULTURE , 1982 .

[17]  F. Watt.  Effect of seeding density on stability of the differentiated phenotype of pig articular chondrocytes in culture. , 1988, Journal of cell science.