High‐Speed Cell Sorting

Publisher Summary High-speed cell sorters are currently available from a handful of manufacturers with varying specifications, capabilities, facility requirements, and user interfaces. The purchase of any one particular instrument will depend on the desires of individual laboratories and should come after careful consideration of these differences. Successful high-speed cell sorting requires both careful preparation of the sample to be studied and maintenance of the instrument being used. In most cases, the upkeep of electronics, lasers, deflexion plates, software, and other hardware is left to service professionals, while alignment of optics and maintenance of fluidics and associated systems remain the purview of the user. Attaching an empty sample tube and sheath container to the sorter and pressurizing each for a brief period can accomplish air drying of the sample and sheath lines. The tubing and the sheath containers should be stored dry in a clean place. To separate the desired fraction of cells, an electrical charge may be applied to the droplets containing cells of interest as they separate from the main jet. In this manner, droplets with different cell types are directed toward collection vials by a static electrical field.

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