Blockade of Cd40-Cd154 Interferes with Human T cell Engraftment in Scid Mice

• Abstract — Antibodies to the ligand for CD40 (CD154) have been shown to exert profound effects on the develop­ ment of cell-mediated immune responses in mice. The present study shows that an antibody to human CD154 (hCD40L) inhibits in vivo Tetanus toxoid (TT) specific secondary antibody responses in hu-PBL-scirf mice, as well as the expansion of xenoreactive human T cells in the scid mice. A possible cause for the reduced expansion of xeno­ reactive, human T cells, was the decreased expression of murine B7.1 and B7.2 caused by the administration of anti-hCD40L. Therefore, it may be that defective matura­ tion of murine antigen-presenting cells impeded the prim­ ing and expansion of human xenoreactive T cells. © 1998 Elsevier Science Inc.

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