A novel electrochemical DNA biosensor for Ebola virus detection.

The aim of this study was to fabricate a novel electrochemical-based DNA-sensing device for Ebola virus DNA diagnostic by an enzyme-amplified detection, which improves the sensitivity and selectivity of the sensor. A thiolated DNA capture probe sequence was immobilized on the screen printed electrode surface and hybridized with biotinylated target strand DNA for the fabrication of Ebola DNA-sensing devices. Prior to the electrochemical detection of the enzymatic product by differential pulse voltammetry (DPV) method, the biotinylated hybrid was labeled with a streptavidin-alkaline phosphatase conjugate on the surface of the working electrode. All the experiment steps were optimized using electrochemical impedance spectroscopy (EIS) and the optimum condition for biosensor fabrication was achieved. A detection limit of 4.7 nM complementary oligonucleotides was obtained using the fabricated biosensor and the standard deviation of the blank solution. The last step, the selectivity and reproducibility of fabricated electrochemical DNA biosensor was obtained.

[1]  Heinz Feldmann,et al.  Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses , 2005, Nature Medicine.

[2]  Hung-Wei Yang,et al.  An electrochemical biosensor to simultaneously detect VEGF and PSA for early prostate cancer diagnosis based on graphene oxide/ssDNA/PLLA nanoparticles. , 2017, Biosensors & bioelectronics.

[3]  Eric S. McLamore,et al.  Impedance biosensor for the rapid detection of Listeria spp. based on aptamer functionalized Pt-interdigitated microelectrodes array , 2016, SPIE Commercial + Scientific Sensing and Imaging.

[4]  Yunlei Zhou,et al.  Investigation of the effect of phytohormone on the expression of microRNA-159a in Arabidopsis thaliana seedlings based on mimic enzyme catalysis systematic electrochemical biosensor. , 2014, Biosensors & bioelectronics.

[5]  Yongmei Yin,et al.  Rolling circle amplification in electrochemical biosensor with biomedical applications , 2016 .

[6]  Lijun Wu,et al.  Evolved bacterial biosensor for arsenite detection in environmental water. , 2015, Environmental science & technology.

[7]  Yildiz Uludag,et al.  Microfluidics and nanoparticles based amperometric biosensor for the detection of cyanobacteria (Planktothrix agardhii NIVA-CYA 116) DNA. , 2015, Biosensors & bioelectronics.

[8]  J. De Coninck,et al.  Designing a high performance, stable spectroscopic biosensor for the binding of large and small molecules. , 2017, Journal of colloid and interface science.

[9]  C. Zhong,et al.  Nanostructured SERS-electrochemical biosensors for testing of anticancer drug interactions with DNA. , 2016, Biosensors & bioelectronics.

[10]  B. Friedrich,et al.  Potential Vaccines and Post-Exposure Treatments for Filovirus Infections , 2012, Viruses.

[11]  Wei Wei,et al.  Electrochemical biosensor for DNA damage detection based on exonuclease III digestions , 2014 .

[12]  A cancer-targeted drug delivery system developed with gold nanoparticle mediated DNA–doxorubicin conjugates , 2014 .

[13]  Jing-zhi Tian.  A High Sensitive Electrochemical Avian Influenza Virus H7 Biosensor Based on CNTs/MoSx Aerogel , 2017 .

[14]  M. Paknejad,et al.  Analytical characterization of label-free immunosensor subsystems based on multi-walled carbon nanotube array-modified gold interface. , 2015, Combinatorial chemistry & high throughput screening.

[15]  A. Aziz,et al.  Recent advances in DNA-based electrochemical biosensors for heavy metal ion detection: A review. , 2017, Biosensors & bioelectronics.

[16]  Baohong Liu,et al.  A dual-signaling strategy for ultrasensitive detection of bisphenol A by aptamer-based electrochemical biosensor , 2016 .

[17]  F. Romanato,et al.  A surface acoustic wave (SAW)-enhanced grating-coupling phase-interrogation surface plasmon resonance (SPR) microfluidic biosensor. , 2016, Lab on a chip.

[18]  Gorjan Alagic,et al.  #p , 2019, Quantum information & computation.

[19]  M. Ganjali,et al.  The effect of pH on the interaction between Eu3 + ions and short single-stranded DNA sequence, studied with electrochemical, spectroscopic and computational methods , 2012 .

[20]  C. Pallier.  [Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses]. , 2005, Virologie.

[21]  J. Mascola,et al.  Safety and Immunogenicity of DNA Vaccines Encoding Ebolavirus and Marburgvirus Wild-Type Glycoproteins in a Phase I Clinical Trial , 2014, The Journal of infectious diseases.

[22]  G. Marrazza,et al.  Design of an Affibody-Based Recognition Strategy for Human Epidermal Growth Factor Receptor 2 (HER2) Detection by Electrochemical Biosensors , 2016 .

[23]  A. Gulland.  Clinical trials of Ebola therapies to begin in December , 2014, BMJ : British Medical Journal.

[24]  T. Hain,et al.  Complete Genome Sequence of an Ebola Virus Isolate Imported from Sierra Leone to Germany Determined by Circle Sequencing , 2016, Genome Announcements.

[25]  P. Norouzi,et al.  Electrochemical spectroscopic investigations on the interaction of an ytterbium complex with DNA and their analytical applications such as biosensor. , 2011, International journal of biological macromolecules.

[26]  M. Hepel,et al.  Intervention of glutathione in pre-mutagenic catechol-mediated DNA damage in the presence of copper(II) ions. , 2012, Mutation research.

[27]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[28]  Stephan Günther,et al.  Emergence of Zaire Ebola virus disease in Guinea. , 2014, The New England journal of medicine.

[29]  M. Ganjali,et al.  Interaction study of ss-DNA and Yb3+ ions in aqueous solutions by electrochemical and spectroscopic techniques , 2012 .

[30]  Kyoung G. Lee,et al.  Hierarchical porous microspheres of the Co3O4@graphene with enhanced electrocatalytic performance for electrochemical biosensors. , 2017, Biosensors & bioelectronics.

[31]  Yinzhi Zhang,et al.  Enzymatic amplification detection of peanut allergen Ara h1 using a stem-loop DNA biosensor modified with a chitosan-mutiwalled carbon nanotube nanocomposite and spongy gold film. , 2015, Talanta.

[32]  Maria Hepel,et al.  Nanogravimetric and voltammetric DNA-hybridization biosensors for studies of DNA damage by common toxicants and pollutants. , 2010, Biophysical chemistry.

[33]  Gou-Jen Wang,et al.  Polymerase chain reaction-free detection of hepatitis B virus DNA using a nanostructured impedance biosensor. , 2016, Biosensors & bioelectronics.

[34]  Ajeet Kaushik,et al.  Towards detection and diagnosis of Ebola virus disease at point-of-care , 2015, Biosensors and Bioelectronics.

[35]  C. Min,et al.  Investigation of phase SPR biosensor for efficient targeted drug screening with high sensitivity and stability , 2015 .