Abscisic Acid Antagonizes Ethylene-Induced Hyponastic Growth in Arabidopsis1[OA]

Ethylene induces enhanced differential growth in petioles of Arabidopsis (Arabidopsis thaliana), resulting in an upward movement of the leaf blades (hyponastic growth). The amplitude of this effect differs between accessions, with Columbia-0 (Col-0) showing a large response, while in Landsberg erecta (Ler), hyponastic growth is minimal. Abscisic acid (ABA) was found to act as an inhibitory factor of this response in both accessions, but the relationship between ethylene and ABA differed between the two; the ability of ABA to inhibit ethylene-induced hyponasty was significantly more pronounced in Col-0. Mutations in ABI1 or ABI3 induced a strong ethylene-regulated hyponastic growth in the less responsive accession Ler, while the response was abolished in the ABA-hypersensitive era1 in Col-0. Modifications in ABA levels altered petiole angles in the absence of applied ethylene, indicating that ABA influences petiole angles also independently from ethylene. A model is proposed whereby the negative effect of ABA on hyponastic growth is overcome by ethylene in Col-0 but not in Ler. However, when ABA signaling is artificially released in Ler, this regulatory mechanism is bypassed, resulting in a strong hyponastic response in this accession.

[1]  Wenhua Zhang,et al.  A Bifurcating Pathway Directs Abscisic Acid Effects on Stomatal Closure and Opening in Arabidopsis , 2006, Science.

[2]  John Okyere,et al.  How to decide? Different methods of calculating gene expression from short oligonucleotide array data will give different results , 2006, BMC Bioinformatics.

[3]  L. Voesenek,et al.  The stimulating effects of ethylene and auxin on petiole elongation and on hyponastic curvature are independent processes in submerged Rumex palustris. , 2006, Plant, cell & environment.

[4]  R. Hill,et al.  The RNA-binding protein FCA is an abscisic acid receptor , 2006, Nature.

[5]  J. Benschop,et al.  Contrasting interactions between ethylene and abscisic acid in Rumex species differing in submergence tolerance. , 2005, The Plant journal : for cell and molecular biology.

[6]  Frank F. Millenaar,et al.  Ethylene-Induced Differential Growth of Petioles in Arabidopsis. Analyzing Natural Variation, Response Kinetics, and Regulation1 , 2005, Plant Physiology.

[7]  J. Benschop,et al.  The Roles of Ethylene, Auxin, Abscisic Acid, and Gibberellin in the Hyponastic Growth of Submerged Rumex palustris Petioles1 , 2004, Plant Physiology.

[8]  D. Van Der Straeten,et al.  Transcriptional profiling by cDNA-AFLP and microarray analysis reveals novel insights into the early response to ethylene in Arabidopsis. , 2004, The Plant journal : for cell and molecular biology.

[9]  Mark Stitt,et al.  Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. , 2004, The Plant journal : for cell and molecular biology.

[10]  R. E. Sharp,et al.  Maintenance of shoot growth by endogenous ABA: genetic assessment of the involvement of ethylene suppression. , 2003, Journal of experimental botany.

[11]  E. Grill,et al.  Relay and control of abscisic acid signaling. , 2003, Current opinion in plant biology.

[12]  Filip Vandenbussche,et al.  Ethylene and Auxin Control the Arabidopsis Response to Decreased Light Intensity1 , 2003, Plant Physiology.

[13]  R. Pierik,et al.  Ethylene is required in tobacco to successfully compete with proximate neighbours , 2003 .

[14]  Frank F. Millenaar,et al.  Plant Movement. Submergence-Induced Petiole Elongation inRumex palustris Depends on Hyponastic Growth1 , 2003, Plant Physiology.

[15]  Peter McCourt,et al.  The ABSCISIC ACID INSENSITIVE 3 (ABI3) gene is modulated by farnesylation and is involved in auxin signaling and lateral root development in Arabidopsis. , 2003, The Plant journal : for cell and molecular biology.

[16]  Rafael A Irizarry,et al.  Exploration, normalization, and summaries of high density oligonucleotide array probe level data. , 2003, Biostatistics.

[17]  A. Moorman,et al.  Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data , 2003, Neuroscience Letters.

[18]  T. Speed,et al.  Summaries of Affymetrix GeneChip probe level data. , 2003, Nucleic acids research.

[19]  P. McCourt,et al.  A screen for genes that function in abscisic acid signaling in Arabidopsis thaliana. , 2002, Genetics.

[20]  R. Finkelstein,et al.  Abscisic Acid Signaling in Seeds and Seedlings Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010441. , 2002, The Plant Cell Online.

[21]  Zhenbiao Yang.  Small GTPases: versatile signaling switches in plants. , 2002, The Plant cell.

[22]  J. Tzen,et al.  Steroleosin, a sterol-binding dehydrogenase in seed oil bodies. , 2002, Plant physiology.

[23]  Xuemin Wang,et al.  The Arabidopsis Phospholipase D Family. Characterization of a Calcium-Independent and Phosphatidylcholine-Selective PLDζ1 with Distinct Regulatory Domains1 , 2002, Plant Physiology.

[24]  M. Jackson.  Long-distance signalling from roots to shoots assessed: the flooding story. , 2002, Journal of experimental botany.

[25]  C. Kao,et al.  Maize VP1 complements Arabidopsis abi3 and confers a novel ABA/auxin interaction in roots. , 2002, The Plant journal : for cell and molecular biology.

[26]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[27]  J. Görlach,et al.  Growth Stage–Based Phenotypic Analysis of Arabidopsis , 2001, The Plant Cell Online.

[28]  K. Grossmann,et al.  Auxin-induced ethylene triggers abscisic acid biosynthesis and growth inhibition. , 2000, Plant physiology.

[29]  P. Weisbeek,et al.  The Arabidopsis SUCROSE UNCOUPLED-6 gene is identical to ABSCISIC ACID INSENSITIVE-4: involvement of abscisic acid in sugar responses. , 2000, The Plant journal : for cell and molecular biology.

[30]  P. McCourt,et al.  Regulation of Abscisic Acid Signaling by the Ethylene Response Pathway in Arabidopsis , 2000, Plant Cell.

[31]  J. Giraudat,et al.  Interactions between Abscisic Acid and Ethylene Signaling Cascades , 2000, Plant Cell.

[32]  A. Webb,et al.  ABI1 Protein Phosphatase 2C Is a Negative Regulator of Abscisic Acid Signaling , 1999, Plant Cell.

[33]  J. Schroeder,et al.  Arabidopsis abi1-1 and abi2-1 Phosphatase Mutations Reduce Abscisic Acid–Induced Cytoplasmic Calcium Rises in Guard Cells , 1999, Plant Cell.

[34]  M. Montagu,et al.  The ABSCISIC ACID‐INSENSITIVE 3 (ABI3) gene is expressed during vegetative quiescence processes in Arabidopsis , 1999 .

[35]  C. Ballaré,et al.  Keeping up with the neighbours: phytochrome sensing and other signalling mechanisms. , 1999, Trends in plant science.

[36]  J. Sheen.  Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[37]  S. Merlot,et al.  The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. , 1997, The Plant cell.

[38]  F. Parcy,et al.  Interactions between the ABI1 and the ectopically expressed ABI3 genes in controlling abscisic acid responses in Arabidopsis vegetative tissues. , 1997, The Plant journal : for cell and molecular biology.

[39]  S. Jacobsen,et al.  Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. , 1996, The Plant journal : for cell and molecular biology.

[40]  Peter Engström,et al.  The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid. , 1996, The Plant journal : for cell and molecular biology.

[41]  W. Peacock,et al.  Abscisic Acid Induces the Alcohol Dehydrogenase Gene in Arabidopsis , 1996, Plant physiology.

[42]  F. Parcy,et al.  Regulation of gene expression programs during Arabidopsis seed development: roles of the ABI3 locus and of endogenous abscisic acid. , 1994, The Plant cell.

[43]  J. Cushman,et al.  Characterization and Expression of Photosystem II Genes (psbE, psbF, and psbL) from the Facultative Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum , 1994, Plant physiology.

[44]  J. Giraudat,et al.  Drought Rhizogenesis in Arabidopsis thaliana (Differential Responses of Hormonal Mutants) , 1994, Plant physiology.

[45]  H. Goodman,et al.  Isolation of the Arabidopsis ABI3 gene by positional cloning. , 1992, The Plant cell.

[46]  S. Hoffmann-Benning,et al.  On the role of abscisic Acid and gibberellin in the regulation of growth in rice. , 1992, Plant physiology.

[47]  C. Rock,et al.  The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[48]  S. J. Croker,et al.  An homogeneous radioimmunoassay for abscisic acid using a scintillation proximity assay technique , 1991 .

[49]  E. Nilsen.  The relationship between freezing tolerance and thermotropic leaf movement in five Rhododendron species , 1991, Oecologia.

[50]  C. M. Karssen,et al.  The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana , 1984 .

[51]  J. Zeevaart.  Metabolism of Abscisic Acid and Its Regulation in Xanthium Leaves during and after Water Stress. , 1983, Plant physiology.

[52]  M. Cox.  Plant movement; kinetics and hormonal regulation of hyponastic growth and petiole elongation , 2004 .

[53]  J. Zeevaart.  Metabolism and physiology of abscisic acid , 1988 .