Morinda officinalis polysaccharide attenuates osteoporosis in rats underwent bilateral ovariectomy by suppressing the PGC-1α/PPARγ pathway

Objective Osteoporosis (OP) is a widespread disease that causes risks of spine and hip fractures. Morinda officinalis polysaccharide (MOP) shows therapeutic potential in OP. This article intended to understand the mechanism by which MOP impacts bone mineral density (BMD) and serum trace elements in OP rats. Methods OP rat models were established by bilateral ovariectomy (OVX). Rats were intragastrically administered with MOP or ZLN005 [the activator of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α)] since the first day after operation for 8 weeks. Microstructural changes in OP rats were analyzed using micro-computed tomography system. Contents of serum Zn, Cu, Fe, and Mg in rats were measured. Levels of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), GSH, and malondialdehyde (MDA) in rats were determined by Enzyme-linked immunosorbent assay. Protein levels of PGC-1α and peroxisome proliferator-activated receptor γ (PPARγ) in cartilage tissues of rats were determined via Western blotting. Results MOP enhanced BMD, bone volume per trabecular volume (BV/TV), Tb.N, and Tb.Th and reduced Tb.Sp in the distal femur of OVX rats, elevated levels of serum Cu, Fe, and Mg and contents of SOD, GSH, and GSH-PX and decreased MDA content. Moreover, MOP suppressed the PGC-1α/PPARγ pathway. Activation of PGC-1α partially abolished the action of MOP on ameliorating OP in OVX rats and strengthening anti-oxidation ability. Conclusion MOP mitigated OP in OVX rats by inhibiting the PGC-1α/PPARγ pathway.

[1]  Hui-Jun Li,et al.  Discovering the main "reinforce kidney to strengthening Yang" active components of salt Morinda officinalis based on the spectrum-effect relationship combined with chemometric methods. , 2021, Journal of pharmaceutical and biomedical analysis.

[2]  R. Kagan,et al.  Hormone therapy for postmenopausal osteoporosis management , 2021, Climacteric : the journal of the International Menopause Society.

[3]  Yukio Nakamura,et al.  Zinc Pharmacotherapy for Elderly Osteoporotic Patients with Zinc Deficiency in a Clinical Setting , 2021, Nutrients.

[4]  Jeff S. Kimball,et al.  Oxidative Stress and Osteoporosis. , 2021, The Journal of bone and joint surgery. American volume.

[5]  B. Rashidkhani,et al.  Oxidative balance score and risk of osteoporosis among postmenopausal Iranian women , 2021, Archives of Osteoporosis.

[6]  J. Zwerina,et al.  Longitudinal Changes of Circulating miRNAs During Bisphosphonate and Teriparatide Treatment in an Animal Model of Postmenopausal Osteoporosis , 2021, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[7]  Fulong Zhao,et al.  Correlation of oxidative stress-related biomarkers with postmenopausal osteoporosis: a systematic review and meta-analysis , 2021, Archives of Osteoporosis.

[8]  Ke Yue,et al.  Morinda officinalis polysaccharides improve meat quality by reducing oxidative damage in chickens suffering from tibial dyschondroplasia. , 2020, Food chemistry.

[9]  Chengzhi Lu,et al.  SirT3 activates AMPK-related mitochondrial biogenesis and ameliorates sepsis-induced myocardial injury , 2020, Aging.

[10]  Ammar A. Alsheghri,et al.  Composition and characteristics of trabecular bone in osteoporosis and osteoarthritis. , 2020, Bone.

[11]  Chunyan Yan,et al.  Bioassay-guided isolation and evaluation of anti-osteoporosis active polysaccharides from Morinda officinalis. , 2020, Journal of ethnopharmacology.

[12]  T. Khurana,et al.  Functional effects of muscle PGC-1alpha in aged animals , 2020, Skeletal Muscle.

[13]  J. Tao,et al.  Nicotinamide mononucleotide attenuates glucocorticoid-induced osteogenic inhibition by regulating the SIRT1/PGC-1α signaling pathway , 2020, Molecular medicine reports.

[14]  A. Qian,et al.  Senile Osteoporosis: The Involvement of Differentiation and Senescence of Bone Marrow Stromal Cells , 2020, International journal of molecular sciences.

[15]  Yier Xu,et al.  MiR‑151a-3p promotes postmenopausal osteoporosis by targeting SOCS5 and activating JAK2/STAT3 signaling. , 2020, Rejuvenation research.

[16]  B. Nogueira,et al.  Telmisartan use in rats with preexisting osteoporotics bone disorders increases bone microarchitecture alterations via PPARγ. , 2019, Life sciences.

[17]  Chunyan Yan,et al.  Identification and characterization of a polysaccharide from the roots of Morinda officinalis, as an inducer of bone formation by up-regulation of target gene expression. , 2019, International journal of biological macromolecules.

[18]  S. Robertson,et al.  Current Progress on MicroRNA-Based Gene Delivery in the Treatment of Osteoporosis and Osteoporotic Fracture , 2019, International journal of endocrinology.

[19]  Zhenwen Zhang,et al.  The decline of whole-body glucose metabolism in ovariectomized rats , 2018, Experimental Gerontology.

[20]  J. Reseland,et al.  Deletion of the Transcription Factor PGC-1α in Mice Negatively Regulates Bone Mass , 2018, Calcified Tissue International.

[21]  J. Reseland,et al.  Deletion of the Transcription Factor PGC-1α in Mice Negatively Regulates Bone Mass , 2018, Calcified Tissue International.

[22]  Jiandie D. Lin,et al.  PGC-1α Controls Skeletal Stem Cell Fate and Bone-Fat Balance in Osteoporosis and Skeletal Aging by Inducing TAZ. , 2018, Cell stem cell.

[23]  V. Levin,et al.  Estrogen therapy for osteoporosis in the modern era , 2018, Osteoporosis International.

[24]  G. Wang,et al.  Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis. , 2017, Molecular medicine reports.

[25]  Xiao-feng He,et al.  Berberine alleviates oxidative stress in rats with osteoporosis through receptor activator of NF-kB/receptor activator of NF-kB ligand/osteoprotegerin (RANK/RANKL/OPG) pathway. , 2017, Bosnian journal of basic medical sciences.

[26]  S. Khosla,et al.  Osteoporosis treatment: recent developments and ongoing challenges. , 2017, The lancet. Diabetes & endocrinology.

[27]  C. Cooper,et al.  Recent advances in the pathogenesis and treatment of osteoporosis. , 2015, Clinical medicine.

[28]  K. Insogna,et al.  Bone Health and Osteoporosis. , 2015, Endocrinology and metabolism clinics of North America.

[29]  J. Westendorf,et al.  Osteoporosis and Osteoarthritis , 2015, Methods in Molecular Biology.

[30]  Xiaoyu Xu,et al.  Effect of Morinda officinalis capsule on osteoporosis in ovariectomized rats. , 2014, Chinese journal of natural medicines.

[31]  C. Cervellati,et al.  Oxidative Stress and Bone Resorption Interplay as a Possible Trigger for Postmenopausal Osteoporosis , 2014, BioMed research international.

[32]  J. Aaseth,et al.  Osteoporosis and trace elements--an overview. , 2012, Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements.

[33]  D. Medeiros,et al.  Mitochondrial and sarcoplasmic protein changes in hearts from copper-deficient rats: up-regulation of PGC-1alpha transcript and protein as a cause for mitochondrial biogenesis in copper deficiency. , 2009, The Journal of nutritional biochemistry.

[34]  Zhu Mengyong,et al.  Protective effect of polysaccharides from morinda officinalis on bone loss in ovariectomized rats. , 2008, International journal of biological macromolecules.