Lack of association between Borna disease virus infection and neurological disorders among HIV-infected individuals.

Viruses have been proposed as etiologic cofactors in the pathogenesis of HIV-related neurological disease. To investigate the etiologic potential of Borna disease virus (BDV) in these disorders, two populations were studied: (1) 27 prospectively identified patients with various neurological disorders were evaluated with BDV RT-PCR (CSF, PBMC), and BDV serology, and (2) a separate group of 25 retrospectively studied patients with AIDS dementia complex was evaluated using BDV serology only. A novel, BDV p40 gene RT-PCR assay was developed: conserved primers were used in a non-nested amplification, detecting less than 100 BDV RNA copies and all of nine wild-type, confirmed animal BDV infections. BDV seroprevalences were 12.5% and 8.0%, respectively, which are similar to the general HIV-infected population. None of the prospectively studied patients had detectable BDV RNA in their CSF or PBMC. Our findings do not support the hypothesis that BDV infections are responsible for HIV-related neurological disorders.

[1]  L. Stitz,et al.  No Borna disease virus-specific RNA detected in blood from psychiatric patients in different regions of Germany , 1997, The Lancet.

[2]  K. Arima,et al.  Detection of Borna disease virus genome in normal human brain tissue , 1997, Brain Research.

[3]  M. Salvatore,et al.  Borna disease virus in brains of North American and European people with schizophrenia and bipolar disorder , 1997, The Lancet.

[4]  M. Salvatore,et al.  Borna disease virus in brains of North American and European people with schizophrenia and bipolar disorder. Bornavirus Study Group. , 1997, Lancet.

[5]  H. Feldmann,et al.  Failure to detect Borna disease virus infection in peripheral blood leukocytes from humans with psychiatric disorders. , 1997, Journal of neurovirology.

[6]  L. De,et al.  Detection of Borna disease virus (BDV) antibodies and BDV RNA in psychiatric patients: evidence for high sequence conservation of human blood-derived BDV RNA , 1996, Journal of virology.

[7]  C. Eng.  Seminars in medicine of the Beth Israel Hospital, Boston. The RET proto-oncogene in multiple endocrine neoplasia type 2 and Hirschsprung's disease. , 1996, New England Journal of Medicine.

[8]  E. Masliah,et al.  Detection of borna disease virus antigen and RNA in human autopsy brain samples from neuropsychiatric patients. , 1996, Virology.

[9]  K. Ikuta,et al.  Unusually high seroprevalence of Borna disease virus in clade E human immunodeficiency virus type 1-infected patients with sexually transmitted diseases in Thailand , 1996, Clinical and diagnostic laboratory immunology.

[10]  K. Ikuta,et al.  Demonstration of Borna disease virus RNA in peripheral blood mononuclear cells derived from Japanese patients with chronic fatigue syndrome , 1996, FEBS letters.

[11]  K. Ikuta,et al.  Demonstration of human Borna disease virus RNA in human peripheral blood mononuclear cells , 1995, FEBS letters.

[12]  Howard E. Gendelman,et al.  Dementia Associated with the Acquired Immunodeficiency Syndrome , 1995 .

[13]  A. Metzler,et al.  Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR , 1995, Journal of clinical microbiology.

[14]  W. Zimmermann,et al.  Borna disease virus genome transcribed and expressed in psychiatric patients , 1995, Nature Medicine.

[15]  R. Yolken,et al.  Polymerase Chain Reaction (PCR) Search for Viral Nucleic Acid Sequences in Schizophrenia , 1995, British Journal of Psychiatry.

[16]  S. Lipton,et al.  Seminars in medicine of the Beth Israel Hospital, Boston. Dementia associated with the acquired immunodeficiency syndrome. , 1995, The New England journal of medicine.

[17]  L. Stitz,et al.  Borna disease in naturally infected cattle. , 1994, Journal of comparative pathology.

[18]  T. Briese,et al.  Genomic organization of Borna disease virus. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[19]  L. De,et al.  Sequence and genome organization of Borna disease virus , 1994, Journal of virology.

[20]  W. Zimmermann,et al.  Detection of Borna disease virus RNA in naturally infected animals by a nested polymerase chain reaction. , 1994, Journal of virological methods.

[21]  J. Clements,et al.  Genomic organization of the structural proteins of borna disease virus revealed by a cDNA clone encoding the 38-kDa protein. , 1993, Virology.

[22]  R. Yolken,et al.  Borna disease virus in peripheral blood mononuclear and bone marrow cells of neonatally and chronically infected rats , 1993, Journal of Neuroimmunology.

[23]  J. Clements,et al.  Borna disease virus: nature of the etiologic agent and significance of infection in man. , 1993, Archives of virology. Supplementum.

[24]  N. Lapointe,et al.  Improved detection of HIV-1 envelope sequences using optimized PCR and inosine-substituted primers. , 1991, Molecular and cellular probes.

[25]  J. Amsterdam,et al.  BORNA DISEASE VIRUS-SPECIFIC ANTIBODIES IN PATIENTS WITH HIV INFECTION AND WITH MENTAL DISORDERS , 1988, The Lancet.