Using light scattering measurements to study the effects of monovalent and divalent cations on alginate aggregates

Light scattering measurements were used to assess the effects of selected divalent and monovalent cations on alginate aggregation in vitro. Alginate, formed with either strontium, calcium or cobalt was partially dissolved with sodium. Calcium-alginate was also partially dissolved with two other monovalent cations, lithium and potassium. Phosphate, when added to a solution containing calcium-alginate, scrubbed alginate-bound calcium as well as free calcium in solution. These findings provide an explanation for an alternative approach for breaking down cell wall alginate.

[1]  J. Beardall,et al.  A Novel Method for Extracting Protoplasts from Large Brown Algae , 1993 .

[2]  L. Chapon.  NEPHELOMETRY AS A METHOD FOR STUDYING THE RELATIONS BETWEEN POLYPHENOLS AND PROTEINS , 1993 .

[3]  G. Braek,et al.  Applications of some algal polysaccharides in biotechnology , 1991 .

[4]  R. Mecham,et al.  Organization and Assembly of Plant and Animal Extracellular Matrix , 1990 .

[5]  D. Butler,et al.  Isolation Conditions for High Yields of Protoplasts from Laminaria saccharina and L. digitata (Phaeophyceae) , 1989 .

[6]  R. A. MORTON,et al.  Optical Methods of Chemical Analysis , 1943, Nature.

[7]  Thomas R. P. Gibb,et al.  Optical Methods of Chemical Analysis , 1942 .

[8]  J. Youatt,et al.  Manganese (Mn2+) reverses the inhibition of fungal growth by EGTA , 1993 .

[9]  M. Guiry,et al.  Seaweed resources in Europe: uses and potential , 1991 .

[10]  W. Laetsch,et al.  A Gelling Carbohydrate in Algal Cell Wall Formation , 1990 .

[11]  G. Skjåk-Bræk,et al.  Studies on the Influence of Nutrients on the Composition and Structure of Alginate in Laminaria saccharina (L.) Lamour. (Laminariales, Phaeophyceae) , 1990 .

[12]  O. Smidsrod,et al.  Molecular basis for some physical properties of alginates in the gel state , 1974 .

[13]  R. Chester,et al.  Introduction to marine chemistry , 1971 .

[14]  S. Gronowitz,et al.  A Light Scattering Study of Alginate. , 1968 .

[15]  S. Gronowitz,et al.  The Effect of Divalent Metals on the Properties of Alginate Solutions. II. Comparison of Different Metal Ions. , 1965 .

[16]  S. Gronowitz,et al.  The Effect of Divalent Metals on the Properties of Alginate Solutions. I. Calcium Ions. , 1965 .