Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research

Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon (Salmo salar) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research.

[1]  S. Engelsen,et al.  The plasma metabolome of Atlantic salmon as studied by 1H NMR spectroscopy using standard operating procedures: effect of aquaculture location and growth stage , 2021, Metabolomics.

[2]  P. Schrotz-King,et al.  Evaluation of different stool extraction methods for metabolomics measurements in human faecal samples , 2020, medRxiv.

[3]  S. Grove,et al.  Quantification of Defensive Proteins in Skin Mucus of Atlantic salmon Using Minimally Invasive Sampling and High-Sensitivity ELISA , 2020, Animals : an open access journal from MDPI.

[4]  Pei-chen Huang,et al.  Energy and nitrogenous waste from glutamate/glutamine catabolism facilitates acute osmotic adjustment in non-neuroectodermal branchial cells , 2020, Scientific Reports.

[5]  M. Izquierdo,et al.  Stress response and skin mucus production of greater amberjack (Seriola dumerili) under different rearing conditions , 2020 .

[6]  Santiago Lamas,et al.  Data-dependent normalization strategies for untargeted metabolomics—a case study , 2020, Analytical and Bioanalytical Chemistry.

[7]  Biswapriya B Misra,et al.  Data normalization strategies in metabolomics: Current challenges, approaches, and tools , 2020, European journal of mass spectrometry.

[8]  C. Fæste,et al.  Proteomic profiling of salmon skin mucus for the comparison of sampling methods. , 2019, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[9]  John A. Bowden,et al.  International Ring Trial of a High Resolution Targeted Metabolomics and Lipidomics Platform for Serum and Plasma Analysis. , 2019, Analytical chemistry.

[10]  D. Wishart.  Metabolomics for Investigating Physiological and Pathophysiological Processes. , 2019, Physiological reviews.

[11]  A. Alfaro,et al.  Impact of acute handling stress, anaesthesia, and euthanasia on fish plasma biochemistry: implications for veterinary screening and metabolomic sampling , 2019, Fish Physiology and Biochemistry.

[12]  D. Macqueen,et al.  Rainbow trout (Oncorhynchus mykiss) urea cycle and polyamine synthesis gene families show dynamic expression responses to inflammation , 2019, Fish & shellfish immunology.

[13]  S. Dong,et al.  Metabolic responses in the gills of tongue sole (Cynoglossus semilaevis) exposed to salinity stress using NMR-based metabolomics. , 2019, The Science of the total environment.

[14]  Yun-Long Zhang,et al.  Partial Amino Acid Metabolism and Glutamine Synthesis as the Ammonia Defensive Strategies During Aerial Exposure in Chinese Loach Paramisgurnus dabryanus , 2019, Front. Physiol..

[15]  Ignasi Sanahuja,et al.  Skin mucus metabolites and cortisol in meagre fed acute stress-attenuating diets: Correlations between plasma and mucus , 2019, Aquaculture.

[16]  A. Moing,et al.  Metabolomics and fish nutrition: a review in the context of sustainable feed development , 2018, Reviews in Aquaculture.

[17]  D. Lecchini,et al.  Biological and Ecological Roles of External Fish Mucus: A Review , 2018, Fishes.

[18]  Ignasi Sanahuja,et al.  Skin mucus metabolites in response to physiological challenges: A valuable non-invasive method to study teleost marine species. , 2018, The Science of the total environment.

[19]  S. Das,et al.  Epidermal mucus, a major determinant in fish health: a review. , 2018, Iranian journal of veterinary research.

[20]  A. B. Kristoffersen,et al.  Workflow for the Targeted and Untargeted Detection of Small Metabolites in Fish Skin Mucus , 2018, Fishes.

[21]  A. Alfaro,et al.  Showcasing metabolomic applications in aquaculture: a review , 2018 .

[22]  D. Lecchini,et al.  Fish mucus metabolome reveals fish life-history traits , 2017, Coral Reefs.

[23]  Caroline H. Johnson,et al.  Metabolomics: beyond biomarkers and towards mechanisms , 2016, Nature Reviews Molecular Cell Biology.

[24]  G. Ankley,et al.  Metabolite profiling of fish skin mucus: a novel approach for minimally-invasive environmental exposure monitoring and surveillance. , 2015, Environmental science & technology.

[25]  N. W. Ross,et al.  Changes in Atlantic salmon Salmo salar mucus components following short- and long-term handling stress. , 2010, Journal of fish biology.

[26]  S. Chew,et al.  Ammonia Production, Excretion, Toxicity, and Defense in Fish: A Review , 2010, Front. Physiology.

[27]  Guoyao Wu,et al.  New developments in fish amino acid nutrition: towards functional and environmentally oriented aquafeeds , 2009, Amino Acids.

[28]  J. Oehlenschläger,et al.  Is humane slaughter of fish possible for industry , 2003 .

[29]  K. J. Firth,et al.  Changes in hydrolytic enzyme activities of naïve Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation. , 2000, Diseases of aquatic organisms.

[30]  C. Milligan.  The role of cortisol in amino acid mobilization and metabolism following exhaustive exercise in rainbow trout (Oncorhynchus mykiss Walbaum) , 1997, Fish Physiology and Biochemistry.

[31]  G. Iwama,et al.  The effects of five fish anaesthetics on acid–base balance, hematocrit, blood gases, cortisol, and adrenaline in rainbow trout , 1989 .