Simultaneous photoelectrochemical and visualized immunoassay of β-human chorionic gonadotrophin.

Herein, on the basis of the alkaline phosphate (ALP) induced reaction, a simultaneous photoelectrochemical (PEC) and visualized immunoassay has been established for the detection of β-human chorionic gonadotrophin (β-HCG). Specifically, in the proposed system, ALP stimulated the oxidative hydrolyzing transformation of 5-bromo-4-chloro-3-indoyl phosphate (BCIP) to an indigo precipitation, generating an insulating layer that impeded the interfacial mass and electron transfer and thus the photocurrent production. Meanwhile, a visualized detection could be performed according to the change of color intensity. Upon proper experimental conditions, the protocol possessed a detection range of 0.5-1000IU/L with a detection limit of (0.20±0.011)IU/L toward β-HCG. With high sensitivity and specificity, this work presents the first general protocol for simultaneous PEC and visualized detection, which could be easily extended to addressing numerous other targets.

[1]  Kai Yan,et al.  A Cathodic "Signal-off" Photoelectrochemical Aptasensor for Ultrasensitive and Selective Detection of Oxytetracycline. , 2015, Analytical chemistry.

[2]  Shuyan Niu,et al.  Ultrasensitive photoelectrochemical immunoassay of antibody against tumor-associated carbohydrate antigen amplified by functionalized graphene derivates and enzymatic biocatalytic precipitation. , 2014, Biosensors & bioelectronics.

[3]  M. O'Sullivan,et al.  Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay. , 1981, Methods in enzymology.

[4]  Wei-Wei Zhao,et al.  Ultrasensitive photoelectrochemical sensing of Pb2+ based on allosteric transition of G-Quadruplex DNAzyme , 2013 .

[5]  I. Willner,et al.  Semiconductor quantum dots for bioanalysis. , 2008, Angewandte Chemie.

[6]  Jun‐Jie Zhu,et al.  Ultrasensitive photoelectrochemical immunoassay for matrix metalloproteinase-2 detection based on CdS:Mn/CdTe cosensitized TiO2 nanotubes and signal amplification of SiO2@Ab2 conjugates. , 2014, Analytical chemistry.

[7]  A. Rudnicka,et al.  SURUSS in perspective. , 2005, Seminars in perinatology.

[8]  L. Cole,et al.  Gestational trophoblastic diseases: 2. Hyperglycosylated hCG as a reliable marker of active neoplasia. , 2006, Gynecologic oncology.

[9]  L. Reubsaet,et al.  Immuno-MS based targeted proteomics: highly specific, sensitive, and reproducible human chorionic gonadotropin determination for clinical diagnostics and doping analysis. , 2012, Analytical chemistry.

[10]  Wei-Wei Zhao,et al.  In situ modification of a semiconductor surface by an enzymatic process: a general strategy for photoelectrochemical bioanalysis. , 2013, Analytical chemistry.

[11]  Wei-Wei Zhao,et al.  The coupling of localized surface plasmon resonance-based photoelectrochemistry and nanoparticle size effect: towards novel plasmonic photoelectrochemical biosensing. , 2012, Chemical communications.

[12]  Dianping Tang,et al.  Plasmonic AuNP/g-C3N4 Nanohybrid-based Photoelectrochemical Sensing Platform for Ultrasensitive Monitoring of Polynucleotide Kinase Activity Accompanying DNAzyme-Catalyzed Precipitation Amplification. , 2015, ACS applied materials & interfaces.

[13]  Feng Li,et al.  A versatile immobilization-free photoelectrochemical biosensor for ultrasensitive detection of cancer biomarker based on enzyme-free cascaded quadratic amplification strategy. , 2016, Biosensors & bioelectronics.

[14]  Wei-Wei Zhao,et al.  Highly sensitive photoelectrochemical immunoassay with enhanced amplification using horseradish peroxidase induced biocatalytic precipitation on a CdS quantum dots multilayer electrode. , 2012, Analytical chemistry.

[15]  Wei-Wei Zhao,et al.  Ultrasensitive photoelectrochemical biosensing based on biocatalytic deposition , 2011 .

[16]  Xiaoru Zhang,et al.  Photoelectrochemically active species and photoelectrochemical biosensors , 2013 .

[17]  Zhihui Dai,et al.  Quantum dots sensitized titanium dioxide decorated reduced graphene oxide for visible light excited photoelectrochemical biosensing at a low potential. , 2014, Biosensors & bioelectronics.

[18]  C. Blomqvist,et al.  Free beta-subunit of human chorionic gonadotropin in serum is a diagnostically sensitive marker of seminomatous testicular cancer. , 2008, Clinical chemistry.

[19]  L. Cole,et al.  Hyperglycosylated hCG (invasive trophoblast antigen, ITA) a key antigen for early pregnancy detection. , 2003, Clinical biochemistry.

[20]  Wei-Wei Zhao,et al.  Photoelectrochemical DNA biosensors. , 2014, Chemical reviews.

[21]  Xiangwei Zhu,et al.  Sensitive sandwich electrochemical immunosensor for human chorionic gonadotropin using nanoporous Pd as a label , 2014 .

[22]  Chao Ma,et al.  Multiplexed enzyme-free electrochemical immunosensor based on ZnO nanorods modified reduced graphene oxide-paper electrode and silver deposition-induced signal amplification strategy. , 2015, Biosensors & bioelectronics.

[23]  Qing Hao,et al.  "Signal-on" photoelectrochemical sensing strategy based on target-dependent aptamer conformational conversion for selective detection of lead(II) ion. , 2014, ACS applied materials & interfaces.

[24]  Serge Cosnier,et al.  Photoelectrochemical immunosensor for label-free detection and quantification of anti-cholera toxin antibody. , 2006, Journal of the American Chemical Society.

[25]  Songqin Liu,et al.  Label-free photoelectrochemical immunosensor for neutrophil gelatinase-associated lipocalin based on the use of nanobodies. , 2015, Analytical chemistry.

[26]  Wei Wu,et al.  A simple and sensitive immunoassay for the determination of human chorionic gonadotropin by graphene-based chemiluminescence resonance energy transfer. , 2014, Biosensors & bioelectronics.

[27]  Yuming Dong,et al.  An ultrasensitive and universal photoelectrochemical immunoassay based on enzyme mimetics enhanced signal amplification. , 2015, Biosensors & bioelectronics.

[28]  Jing Li,et al.  In situ-generated nano-gold plasmon-enhanced photoelectrochemical aptasensing based on carboxylated perylene-functionalized graphene. , 2014, Analytical chemistry.

[29]  Wei-Wei Zhao,et al.  In situ enzymatic ascorbic acid production as electron donor for CdS quantum dots equipped TiO2 nanotubes: a general and efficient approach for new photoelectrochemical immunoassay. , 2012, Analytical chemistry.

[30]  Jing Li,et al.  Photoelectrochemical biosensor using enzyme-catalyzed in situ propagation of CdS quantum dots on graphene oxide. , 2014, ACS applied materials & interfaces.

[31]  Jing Wang,et al.  Exciton-plasmon interactions between CdS quantum dots and Ag nanoparticles in photoelectrochemical system and its biosensing application. , 2012, Analytical chemistry.

[32]  Ping Huang,et al.  Lanthanide-doped LiLuF(4) upconversion nanoprobes for the detection of disease biomarkers. , 2014, Angewandte Chemie.

[33]  Fan Yu,et al.  A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin. , 2004, Biosensors & bioelectronics.

[34]  Samuel Sánchez,et al.  Toward a fast, easy, and versatile immobilization of biomolecules into carbon nanotube/polysulfone-based biosensors for the detection of hCG hormone. , 2008, Analytical chemistry.

[35]  Peng Wang,et al.  Highly efficient visual detection of trace copper(II) and protein by the quantum photoelectric effect. , 2013, Analytical chemistry.

[36]  T. G. Shrivastav,et al.  Development of Isotopic and Non‐Isotopic Microwell Based Immunoassays for hCG Using 125I and Biotin Labeled hCG , 2005, Journal of immunoassay & immunochemistry.

[37]  Qingming Shen,et al.  Enhanced photoelectrochemical aptasensing platform based on exciton energy transfer between CdSeTe alloyed quantum dots and SiO2@Au nanocomposites. , 2015, Chemical communications.

[38]  P. Prasad,et al.  Development of Colorimetric Enzyme‐Linked Immunosorbent Assay for Human Chorionic Gonadotropin , 2006, Journal of immunoassay & immunochemistry.