Interaction of lycorine with UV-B and kinetin in cucumber (Cucumis sativus L.) Cotyledons

Interaction of lycorine, a strong inhibitor of ascorbic acid biosynthesis with kinetin and UV-B radiation has been studied in cucumber (Cucumis sativus L.) cotyledons. Lycorine inhibited hypocotyl growth of seedlings as well as kinetin induced expansion growth of cotyledons in darkness. Exposure to UV-B radiation has similar inhibitory effect on growth. Inhibition of growth by lycorine was accompanied by inhibition of ascorbic acid biosynthesis but UV-B radiation promoted ascorbic acid synthesis while inhibiting growth. The inhibitory effect of lycorine on ascorbic acid synthesis could be completely recovered by kinetin. The role of ascorbic acid as a growth promoter and as an antioxidant is discussed in the light of lycorine effect.

[1]  V. Singh,et al.  Modulation of manganese toxicity in Pisum sativum L. seedlings by kinetin , 2010 .

[2]  S. Kataria,et al.  UV-B induced changes in antioxidant enzymes and their isoforms in cucumber (Cucumis sativus L.) cotyledons. , 2007, Indian journal of biochemistry & biophysics.

[3]  H. Asard,et al.  Involvement of ascorbic acid and ab-type cytochrome in plant plasma membrane redox reactions , 1995, Protoplasma.

[4]  S. Kataria,et al.  Oxyradicals under UV-b stress and their quenching by antioxidants. , 2004, Indian journal of experimental biology.

[5]  Y. Takeuchi,et al.  Peroxidation of lipids and growth inhibition induced by UV-B irradiation , 1995, Plant Cell Reports.

[6]  S. Kataria,et al.  Changes in antioxidant defenses of cucumber cotyledons in response to UV-B and to the free radical generating compound AAPH , 2003 .

[7]  M. Van Montagu,et al.  Ascorbate biosynthesis in Arabidopsis cell suspension culture. , 1999, Plant physiology.

[8]  K. Guruprasad,et al.  Modulation of a guaiacol peroxidase inhibitor by UV-B in cucumber cotyledons , 1998 .

[9]  L. Gara,et al.  Correlation between changes in cell ascorbate and growth of Lupinus albus seedlings , 1997 .

[10]  P. Navas,et al.  Role of Apoplastic and Cell-Wall Peroxidases on the Stimulation of Root Elongation by Ascorbate , 1996, Plant physiology.

[11]  G. Paliyath,et al.  Ultraviolet-B- and Ozone-Induced Biochemical Changes in Antioxidant Enzymes of Arabidopsis thaliana , 1996, Plant physiology.

[12]  D. Ormrod,et al.  IMPACT OF UVB and 03 ON THE OXYGEN FREE RADICAL SCAVENGING SYSTEM IN Arabidopsis thaliana GENOTYPES DIFFERING IN FLAVONOID BIOSYNTHESIS , 1995 .

[13]  S. Sgorbati,et al.  Ascorbic acid effect on the onset of cell proliferation in pea root , 1994 .

[14]  L. Gara,et al.  «In vivo» Inhibition of Galactono-γ-Lactone Conversion to Ascorbate by Lycorine , 1994 .

[15]  L. Gara,et al.  Changes in the Ascorbate System during Seed Development of Vicia faba L. , 1992, Plant physiology.

[16]  L. Gara,et al.  The biogenesis of galactone-γ-lactone oxidase in Avena sativa embryos , 1992 .

[17]  L. Gara,et al.  Ascorbic acid utilization by prolyl hydroxylase in vivo , 1991 .

[18]  O. Arrigoni,et al.  The size of quiescent centre in roots of Allium cepa L. grown with ascorbic acid. , 1990, The New phytologist.

[19]  O. Arrigoni,et al.  Lycorine as an inhibitor of ascorbic acid biosynthesis , 1975, Nature.

[20]  M. Laloraya,et al.  Cucumber cotyledon expansion as a bioassay for cytokinins , 1974 .

[21]  G. Dalessandro,et al.  Inhibitory effect of lycorine on cell division and cell elongation , 1973 .