Potential applications of flat-panel volumetric CT in morphologic and functional small animal imaging.

Noninvasive radiologic imaging has recently gained considerable interest in basic and preclinical research for monitoring disease progression and therapeutic efficacy. In this report, we introduce flat-panel volumetric computed tomography (fpVCT) as a powerful new tool for noninvasive imaging of different organ systems in preclinical research. The three-dimensional visualization that is achieved by isotropic high-resolution datasets is illustrated for the skeleton, chest, abdominal organs, and brain of mice. The high image quality of chest scans enables the visualization of small lung nodules in an orthotopic lung cancer model and the reliable imaging of therapy side effects such as lung fibrosis. Using contrast-enhanced scans, fpVCT displayed the vascular trees of the brain, liver, and kidney down to the subsegmental level. Functional application of fpVCT in dynamic contrast-enhanced scans of the rat brain delivered physiologically reliable data of perfusion and tissue blood volume. Beyond scanning of small animal models as demonstrated here, fpVCT provides the ability to image animals up to the size of primates.

[1]  C. Ruppert,et al.  Changes in pulmonary surfactant function and composition in bleomycin-induced pneumonitis and fibrosis. , 2004, Toxicology and applied pharmacology.

[2]  Martin Obert,et al.  Flat-Panel Volumetric Computed Tomography: A New Method for Visualizing Fine Bone Detail in Living Mice , 2005, Journal of computer assisted tomography.

[3]  J. Boone,et al.  Small-animal X-ray dose from micro-CT. , 2004, Molecular imaging.

[4]  M J Welch,et al.  Small animal imaging: current technology and perspectives for oncological imaging , 2002 .

[5]  Wolfhard Semmler,et al.  Volumetric computed tomography (VCT): a new technology for noninvasive, high-resolution monitoring of tumor angiogenesis , 2004, Nature Medicine.

[6]  S. Merajver,et al.  Radiotherapy and antiangiogenic TM in lung cancer. , 2002, Neoplasia.

[7]  M J Paulus,et al.  High resolution X-ray computed tomography: an emerging tool for small animal cancer research. , 2000, Neoplasia.

[8]  H. Tsukada,et al.  Anti‐neovascular therapy by liposomal drug targeted to membrane type‐1 matrix metalloproteinase , 2004, International journal of cancer.

[9]  D W Holdsworth,et al.  Fundamental image quality limits for microcomputed tomography in small animals. , 2003, Medical physics.

[10]  J. Hirsch Imaging and biological function in health and disease. , 2003, The Journal of clinical investigation.

[11]  Z. Liao,et al.  Damage and morbidity from pneumonitis after irradiation of partial volumes of mouse lung. , 1995, International journal of radiation oncology, biology, physics.

[12]  R. Kerbel Antiangiogenic drugs and current strategies for the treatment of lung cancer. , 2004, Seminars in oncology.

[13]  Ting-Yim Lee,et al.  Practical injection-rate CT perfusion imaging: deconvolution-derived hemodynamics in a case of stroke , 2001, Neuroradiology.

[14]  T S Smith,et al.  Three‐dimensional microimaging (MRμI and μCT), finite element modeling, and rapid prototyping provide unique insights into bone architecture in osteoporosis , 2001, The Anatomical record.

[15]  L. Schad,et al.  Dynamic T1‐weighted monitoring of vascularization in human carcinoma heterotransplants by magnetic resonance imaging , 2003, International journal of cancer.

[16]  S. Larson,et al.  Imaging transgene expression with radionuclide imaging technologies. , 2000, Neoplasia.

[17]  R. Weissleder Molecular imaging: exploring the next frontier. , 1999, Radiology.

[18]  François Estève,et al.  Absolute Cerebral Blood Volume and Blood Flow Measurements Based on Synchrotron Radiation Quantitative Computed Tomography , 2003, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[19]  M J Paulus,et al.  High resolution computed tomography and MRI for monitoring lung tumor growth in mice undergoing radioimmunotherapy: correlation with histology. , 2000, Medical physics.

[20]  R. Weissleder Scaling down imaging: molecular mapping of cancer in mice , 2002, Nature Reviews Cancer.

[21]  Fabian Kiessling,et al.  Sensitive noninvasive monitoring of tumor perfusion during antiangiogenic therapy by intermittent bolus-contrast power Doppler sonography. , 2003, Cancer research.

[22]  H. Chapman Disorders of lung matrix remodeling. , 2004, The Journal of clinical investigation.

[23]  A. Jacobs,et al.  Functional coexpression of HSV-1 thymidine kinase and green fluorescent protein: implications for noninvasive imaging of transgene expression. , 1999, Neoplasia.

[24]  W A Kalender,et al.  [The use of flat-panel detectors for CT imaging]. , 2003, Der Radiologe.

[25]  R Weissleder,et al.  In vivo imaging of gene and cell therapies. , 2001, Experimental hematology.

[26]  J. Bleby,et al.  Laboratory animal medicine. , 2003, The Veterinary record.

[27]  R Kozak,et al.  CT assessment of cerebral perfusion: experimental validation and initial clinical experience. , 1999, Radiology.

[28]  M. Wintermark,et al.  Dynamic perfusion CT: optimizing the temporal resolution and contrast volume for calculation of perfusion CT parameters in stroke patients. , 2004, AJNR. American journal of neuroradiology.

[29]  M. Duncan,et al.  Mutation of the ectodysplasin-A gene results in bone defects in mice. , 2002, Journal of comparative pathology.

[30]  F. Bauss,et al.  Site‐specific human breast cancer (MDA‐MB‐231) metastases in nude rats: Model characterisation and in vivo effects of ibandronate on tumour growth , 2003, International journal of cancer.