TLC and HPTLC Fingerprint Profiles of Different Bioactive Components from the Tuber of Solena amplexicaulis.

Most of the traditional medicinal plants in India are not scientifically validated. Scientific evaluation along with traditional knowledge is essential to obtain effective drugs for commercial purpose. Solena amplexicaulis belongs to the family, Cucurbitaceae, a traditional medicinal plant species of Tamil Nadu, India is being prescribed to cure various diseases. The present study is to establish the chemical fingerprint through TLC and HPTLC analyses for various secondary metabolites in this species. The TLC and HPTLC studies were carried out as per the methods of Harborne and Wagner et al. The profiles of various individual secondary metabolites were made and developed for authentication. The methanolic tuber extract showed the presence of 5 alkaloids, 6 flavonoids, 2 glycosides, 10 saponins and 7 terpenoids. The development of such fingerprint can be used in differentiation of the species from the adulterant in terms of phytochemical constituents and hence act as biochemical markers in the pharma industry and plant systematic studies.

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