Characterization of Mammalian Selenoproteomes

In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are misannotated. We identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs. The human selenoproteome consists of 25 selenoproteins.

[1]  F. Young.  Biochemistry , 1955, The Indian Medical Gazette.

[2]  M. Berry,et al.  Recognition of UGA as a selenocysteine codon in Type I deiodinase requires sequences in the 3′ untranslated region , 1991, Nature.

[3]  T. Rabbitts,et al.  Molecular cloning of putative odorant-binding and odorant-metabolizing proteins. , 1991, Biochemistry.

[4]  M. Berry,et al.  Knowing when not to stop: selenocysteine incorporation in eukaryotes. , 1996, Trends in biochemical sciences.

[5]  N. Copeland,et al.  Identification of a novel selD homolog from eukaryotes, bacteria, and archaea: is there an autoregulatory mechanism in selenocysteine metabolism? , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[6]  E. Westhof,et al.  A novel RNA structural motif in the selenocysteine insertion element of eukaryotic selenoprotein mRNAs. , 1996, RNA.

[7]  T C Stadtman,et al.  Selenocysteine, identified as the penultimate C-terminal residue in human T-cell thioredoxin reductase, corresponds to TGA in the human placental gene. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[8]  K. Kleene,et al.  Developmental expression, intracellular localization, and selenium content of the cysteine‐rich protein associated with the mitochondrial capsules of mouse sperm , 1996, Molecular reproduction and development.

[9]  F. Ursini,et al.  Dual function of the selenoprotein PHGPx during sperm maturation. , 1999, Science.

[10]  G. Kryukov,et al.  New Mammalian Selenocysteine-containing Proteins Identified with an Algorithm That Searches for Selenocysteine Insertion Sequence Elements* , 1999, The Journal of Biological Chemistry.

[11]  D Gautheret,et al.  Novel Selenoproteins Identified in Silico andin Vivo by Using a Conserved RNA Structural Motif* , 1999, The Journal of Biological Chemistry.

[12]  P. Carbon,et al.  Characterization of mSelB, a novel mammalian elongation factor for selenoprotein translation , 2000, The EMBO journal.

[13]  M. Rayman,et al.  The importance of selenium to human health , 2000, The Lancet.

[14]  J. Harney,et al.  Decoding apparatus for eukaryotic selenocysteine insertion , 2000, EMBO reports.

[15]  B. Carlson,et al.  A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs , 2000, The EMBO journal.

[16]  A. Böck.  Biosynthesis of selenoproteins--an overview. , 2000, BioFactors.

[17]  B. Turnbull,et al.  An analysis of cancer prevention by selenium. , 2001, BioFactors.

[18]  R. Guigó,et al.  In silico identification of novel selenoproteins in the Drosophila melanogaster genome , 2001, EMBO reports.

[19]  D. Haussler,et al.  Assembly of the working draft of the human genome with GigAssembler. , 2001, Genome research.

[20]  Timothy B. Stockwell,et al.  The Sequence of the Human Genome , 2001, Science.

[21]  G. Kryukov,et al.  Selenium Metabolism in Drosophila , 2001, The Journal of Biological Chemistry.

[22]  J. V. Moran,et al.  Initial sequencing and analysis of the human genome. , 2001, Nature.

[23]  Vadim N. Gladyshev,et al.  Mammalian Selenoprotein in Which Selenocysteine (Sec) Incorporation Is Supported by a New Form of Sec Insertion Sequence Element , 2002, Molecular and Cellular Biology.

[24]  G. Kryukov,et al.  Mammalian selenoprotein gene signature: identification and functional analysis of selenoprotein genes using bioinformatics methods. , 2002, Methods in enzymology.

[25]  Vadim N. Gladyshev,et al.  How Selenium Has Altered Our Understanding of the Genetic Code , 2002, Molecular and Cellular Biology.

[26]  S. Orkin,et al.  The use of altered specificity mutants to probe specific protein-protein interactions involved in the activation of GATA-1 target genes. , 2002, Methods.

[27]  宁北芳,et al.  疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A , 2005 .